Metadata associated with genomic and genetic data collected from organisms obtained on BOWLs landers, R/V Oceanus June 22-27, 2014 (BOWLS project) (NCEI Accession 0294395)

This dataset contains biological and survey - biological data collected on R/V Oceanus during cruise OC1406B in the Coastal Waters of Southeast Alaska and British Columbia, Davis Strait, and North Pacific Ocean on 2014-06-22. These data include depth, species, and taxon. The instruments used to collect these data include Automated DNA Sequencer. These data were collected by Kenneth M. Halanych of Auburn University and Craig R. Smith of University of Hawaii at Manoa as part of the "Biodiversity, connectivity and ecosystem function in organic-rich whale-bone and wood-fall habitats in the deep sea (BOWLS)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2023-03-09.

The following is the text of the dataset description provided by BCO-DMO:

Sample log for specimens collected for genomics analyses

Dataset Description:
Acquisition Description:
The investigators deployed four free-vehicle Bone-Wood Landers (BOWLs) as moorings that (1) sink autonomously to the deep-sea floor, (2) expose 9 controlled experimental substrates of whale bone, wood, or inert materials at the seafloor for months to years, and (3) upon acoustic command, enclose each experimental substrate in a sealed 500-micrometer mesh bag and returns to the ocean surface. This new BOWL technology allows controlled quantitative study of biotic colonization, biodiversity, ecosystem function and connectivity for bone, wood and other experimental substrates in the deep sea at relatively low fabrication and ship-time costs.

Genomic methods:
RAD_seq = This is 2b-RAD Single Nucleotide Polymorphism (SNP) data collected with the Wang et al. 2012 protocol. The raw reads will be deposited in NCBI Small Read Archive. The data is Illumina SE 50bp data.

mt_genome = This is Illumina PE 150bp data derive from total genomic DNA extracted from the organisms listed in the spreadsheet. The raw reads will be deposited in NCBI Small Read Archive and the assembled mitochondrial genome will be submitted to NCBI GenBank.

Endosymbiont_genome = This is Illumina PE 150bp data derive from total genomic DNA extracted from the organisms listed in the spreadsheet. The raw reads will be deposited in NCBI Small Read Archive and the assembled endosymbiont genome will be submitted to NCBI GenBank.