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Microplastic concentrations in Scylla Serrata (Giant mud crab) from the Kota mangroves, southwestern coast of India collected during 2022-11-25 (NCEI Accession 0292955)

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This study estimated the concentration of microplastics (i.e. plastics measuring less than 5mm; reported in unit of count per individual and count per gram) in Scylla Serrata (Giant mud crab) from the Kota mangroves, southwestern coast of India collected during 2022-11-25. Microplastics were collected using a cylindrical crab trap. Percentages of microplastics in various size ranges and types are reported. This dataset contains the results from all 9 giant mud crab samples, in a spreadsheet format.

Dataset Citation

Cite as: Valsan, Gokul; Tamrakar, Anjali; Warrier, Anish Kumar (2024). Microplastic concentrations in Scylla Serrata (Giant mud crab) from the Kota mangroves, southwestern coast of India collected during 2022-11-25 (NCEI Accession 0292955). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0292955. Accessed [date].

Dataset Identifiers

ISO 19115-2 Metadata

gov.noaa.nodc:0292955

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Distribution Formats	Excel
Ordering Instructions	Contact NCEI for other distribution options and instructions.
Distributor	NOAA National Centers for Environmental Information +1-301-713-3277 NCEI.Info@noaa.gov
Dataset Point of Contact	NOAA National Centers for Environmental Information ncei.info@noaa.gov

Time Period	2022-11-25 to 2022-11-25
Spatial Bounding Box Coordinates	West: 74.694167 East: 74.694722 South: 13.491111 North: 13.515556
Spatial Coverage Map

General Documentation

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Descriptive Information
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Associated Resources

The NOAA NCEI Global Marine Microplastics Database (1972-present)
- NCEI Collection
  Navigate directly to the URL for data access and direct download.
Valsan, G., Tamrakar, A., & Warrier, A. K. (2024). Microplastics in Scylla Serrata: A baseline study from southwest India. Marine Pollution Bulletin, 200, 116109. https://doi.org/10.1016/j.marpolbul.2024.116109
- https://doi.org/10.1016/j.marpolbul.2024.116109
  related journal article

gov.noaa.nodc:NCEI-Marine-Microplastics

Publication Dates	publication: 2024-05-29
Data Presentation Form	Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status	Complete - production of the data has been completed Historical archive - data has been stored in an offline storage facility
Data Update Frequency	As needed
Supplemental Information	Submission Package ID: 5XFHCF
Purpose	These microplastic concentration data were collected in order to determine their abundance in Scylla Serrata (Giant mud crab) from the Kota mangroves, southwestern coast of India during 2022-11-25
Use Limitations	accessLevel: Public Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.

Dataset Citation	Cite as: Valsan, Gokul; Tamrakar, Anjali; Warrier, Anish Kumar (2024). Microplastic concentrations in Scylla Serrata (Giant mud crab) from the Kota mangroves, southwestern coast of India collected during 2022-11-25 (NCEI Accession 0292955). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0292955. Accessed [date].
Cited Authors	Valsan, Gokul Manipal Institute of Technology Tamrakar, Anjali Manipal Institute of Technology Warrier, Anish Kumar Manipal Institute of Technology
Contributors	Manipal Institute of Technology (MIT)
Resource Providers	Anish Kumar Warrier Manipal Institute of Technology (MIT) Manipal Institute of Technology (MIT)
Points of Contact	Ebenezer Nyadjro Mississippi State University; Northern Gulf Institute (NGI)
Publishers	NOAA National Centers for Environmental Information
Acknowledgments	Related Funding Agency: Department of Science and Technology, Government of India (File No. CRG/2021/004725)

Theme keywords	NODC DATA TYPES THESAURUS biological data microplastic concentration shell parameters (length or weight) SPECIES IDENTIFICATION SPECIES IDENTIFICATION - TOTAL WEIGHT STOMACH CONTENTS - CONTENT WEIGHT STOMACH CONTENTS - DIGESTION NODC OBSERVATION TYPES THESAURUS in situ laboratory analyses WMO_CategoryCode oceanography Global Change Master Directory (GCMD) Science Keywords EARTH SCIENCE > BIOLOGICAL CLASSIFICATION EARTH SCIENCE > OCEANS > WATER QUALITY > SEA SURFACE CONTAMINANTS > MICROPLASTIC CONCENTRATION
Data Center keywords	NODC COLLECTING INSTITUTION NAMES THESAURUS Manipal Institute of Technology NODC SUBMITTING INSTITUTION NAMES THESAURUS Manipal Institute of Technology
Instrument keywords	NODC INSTRUMENT TYPES THESAURUS Fourier-transform infrared (FTIR) spectrometer microscope trap Global Change Master Directory (GCMD) Instrument Keywords FTIR SPECTROMETER > Fourier Transform Infrared Spectrometer MICROSCOPES > MICROSCOPES TRAPS > TRAPS Provider Instruments cylindrical crab trap
Place keywords	NODC SEA AREA NAMES THESAURUS Indian Ocean Global Change Master Directory (GCMD) Location Keywords OCEAN > INDIAN OCEAN
Keywords	NCEI ACCESSION NUMBER 0292955

Use Constraints	Cite as: Valsan, Gokul; Tamrakar, Anjali; Warrier, Anish Kumar (2024). Microplastic concentrations in Scylla Serrata (Giant mud crab) from the Kota mangroves, southwestern coast of India collected during 2022-11-25 (NCEI Accession 0292955). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0292955. Accessed [date].
Access Constraints	Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
Fees	In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.

Lineage information for: dataset
Processing Steps	2024-05-29T21:19:30Z - NCEI Accession 0292955 v1.1 was published.
Output Datasets	NCEI Accession 0292955 v1.1 NCEI Accession 0292955 v1.1 (download) published 2024-05-29T21:19:30Z

Lineage information for: dataset
Processing Steps	Parameter or Variable: microplastic concentration (measured); Units: count per individual; Observation Category: in situ; Sampling Instrument: cylindrical crab trap; Sampling and Analyzing Method: Nine samples of the mud crab species (Scylla serrata) were collected from a fisherman near the Kota mangroves. The fisherman had collected the crab samples with the aid of a cylindrical crab trap which were deployed within the mangroves. The samples were neatly packed in aluminum foil and kept in steel containers to avoid contamination. They were safely transported to the laboratory and stored in a deep freezer until further analysis. Prior to sampling, ethical clearance was obtained from the Institutional Animal Ethics Committee of the Manipal Academy of Higher Education to undertake this study. Before dissecting the mud crabs, the length and width of their carapaces, weight of the whole body were measured. Based on their length and width measurements, all the samples belonged to the adult category. Later, these samples were thoroughly rinsed using pre-sieved (0.1 mm) double distilled water to remove any external contaminants. The carapace was removed, and the gastrointestinal tract (GIT) was carefully dissected with the help of a stainless-steel knife and scissors of surgical grade. The weight of the gastrointestinal tract was noted. The digestion of the biological matter was conducted in two phases. In the first phase, potassium hydroxide (10 %) was added to inside a laminar flow to avoid airborne contamination. After the biological matter broke down, the samples were dried at 50 ◦C and later treated with hydrogen peroxide (30 %) for complete digestion of the biological matter. The clear solution was passed through four sets of sieves (5 mm, 1 mm, 0.3 mm, and 0.1 mm stacked one above the other with the largest mesh size on top and the smallest at the bottom). The residue or material present on the 1 mm, 0.3 mm, and 0.1 mm sieves was carefully transferred to watch glasses and considered for the identification of MPs. Microplastics were identified using a stereo zoom microscope (NIKON SMZ-745-T) at 40× magnification. Under the microscope, parameters such as abundance, shape, size, and color were studied. Only those particles meeting the following criteria were considered MPs: particles that lacked any cellular or organic structure, had uniformly thick fibers, did not break upon prodding with tweezers, changed behavior during a hot needle test, and stained with 1 % Rose Bengal. The polymer composition of 95 microplastics (0.3–5 mm size fraction) was determined using Fourier transform infrared spectroscopy with attenuated total reflectance (FTIR-ATR; Shimadzu IRSpirit). The selection of these 95 microplastics was made to ensure that all the different types of microplastics observed during visual identification can also be characterized by IR spectroscopy. Percent transmission was recorded across a spectrum of 3500 to 500 cm-1 using a resolution of 4 cm-1. Prior to scanning, the ATR crystal was carefully cleaned with ethanol, after which the MP was scanned around 25 times. The IR spectra obtained for the samples were matched with reference spectral curves on Open Specy, an open-source software for identifying spectral signatures of polymers (Pearson correlation coefficient > 0.8). The surface morphological features of four fibers which were frequently observed in the gut of the crab were studied using a scanning electron microscope (EVO MA18) at the Innovation Centre, Manipal Institute of Technology.; Data Quality Method: The analysis was conducted in a clean laboratory setting with minimal disturbance from other personnel. All solutions used in the analysis were prepared using double-distilled water (DDW). Only stainless steel and glass apparatus were used during the analysis. All beakers were thoroughly cleaned with DDW and covered with aluminum foil to avoid atmospheric contamination. All the liquids, such as DDW, and all solutions used during analysis were pre-sieved to avoid any external contamination. Nitrile gloves and cotton laboratory coats were used throughout the duration of the experiments. Four laboratory blanks were kept to check the atmospheric contamination inside the laboratory.. Parameter or Variable: microplastic concentration (measured); Units: count per gram; Observation Category: in situ; Sampling Instrument: cylindrical crab trap.

Lineage information for: dataset

Processing Steps

Parameter or Variable: microplastic concentration (measured); Units: count per individual; Observation Category: in situ; Sampling Instrument: cylindrical crab trap; Sampling and Analyzing Method: Nine samples of the mud crab species (Scylla serrata) were collected from a fisherman near the Kota mangroves. The fisherman had collected the crab samples with the aid of a cylindrical crab trap which were deployed within the mangroves. The samples were neatly packed in aluminum foil and kept in steel containers to avoid contamination. They were safely transported to the laboratory and stored in a deep freezer until further analysis. Prior to sampling, ethical clearance was obtained from the Institutional Animal Ethics Committee of the Manipal Academy of Higher Education to undertake this study. Before dissecting the mud crabs, the length and width of their carapaces, weight of the whole body were measured. Based on their length and width measurements, all the samples belonged to the adult category. Later, these samples were thoroughly rinsed using pre-sieved (0.1 mm) double distilled water to remove any external contaminants. The carapace was removed, and the gastrointestinal tract (GIT) was carefully dissected with the help of a stainless-steel knife and scissors of surgical grade. The weight of the gastrointestinal tract was noted. The digestion of the biological matter was conducted in two phases. In the first phase, potassium hydroxide (10 %) was added to inside a laminar flow to avoid airborne contamination. After the biological matter broke down, the samples were dried at 50 ◦C and later treated with hydrogen peroxide (30 %) for complete digestion of the biological matter. The clear solution was passed through four sets of sieves (5 mm, 1 mm, 0.3 mm, and 0.1 mm stacked one above the other with the largest mesh size on top and the smallest at the bottom). The residue or material present on the 1 mm, 0.3 mm, and 0.1 mm sieves was carefully transferred to watch glasses and considered for the identification of MPs. Microplastics were identified using a stereo zoom microscope (NIKON SMZ-745-T) at 40× magnification. Under the microscope, parameters such as abundance, shape, size, and color were studied. Only those particles meeting the following criteria were considered MPs: particles that lacked any cellular or organic structure, had uniformly thick fibers, did not break upon prodding with tweezers, changed behavior during a hot needle test, and stained with 1 % Rose Bengal. The polymer composition of 95 microplastics (0.3–5 mm size fraction) was determined using Fourier transform infrared spectroscopy with attenuated total reflectance (FTIR-ATR; Shimadzu IRSpirit). The selection of these 95 microplastics was made to ensure that all the different types of microplastics observed during visual identification can also be characterized by IR spectroscopy. Percent transmission was recorded across a spectrum of 3500 to 500 cm-1 using a resolution of 4 cm-1. Prior to scanning, the ATR crystal was carefully cleaned with ethanol, after which the MP was scanned around 25 times. The IR spectra obtained for the samples were matched with reference spectral curves on Open Specy, an open-source software for identifying spectral signatures of polymers (Pearson correlation coefficient > 0.8). The surface morphological features of four fibers which were frequently observed in the gut of the crab were studied using a scanning electron microscope (EVO MA18) at the Innovation Centre, Manipal Institute of Technology.; Data Quality Method: The analysis was conducted in a clean laboratory setting with minimal disturbance from other personnel. All solutions used in the analysis were prepared using double-distilled water (DDW). Only stainless steel and glass apparatus were used during the analysis. All beakers were thoroughly cleaned with DDW and covered with aluminum foil to avoid atmospheric contamination. All the liquids, such as DDW, and all solutions used during analysis were pre-sieved to avoid any external contamination. Nitrile gloves and cotton laboratory coats were used throughout the duration of the experiments. Four laboratory blanks were kept to check the atmospheric contamination inside the laboratory..
Parameter or Variable: microplastic concentration (measured); Units: count per gram; Observation Category: in situ; Sampling Instrument: cylindrical crab trap.

Acquisition Information (collection)
Instrument	Fourier-transform infrared (FTIR) spectrometer microscope trap

Last Modified: 2024-05-30T06:21:08Z
For questions about the information on this page, please email: ncei.info@noaa.gov

Microplastic concentrations in Scylla Serrata (Giant mud crab) from the Kota mangroves, southwestern coast of India collected during 2022-11-25 (NCEI Accession 0292955)

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