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Microplastic concentrations in the digestive tracts of Scyliorhinus canicula (small-spotted catshark) caught off the coast of Calp (Alicante, Spain) from 2019-11-01 to 2020-11-01 (NCEI Accession 0292552)

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This study estimated the concentration of microplastics (i.e. plastics measuring less than 5 mm; reported in unit of count per individual) in the digestive tracts of Scyliorhinus canicula (small-spotted catshark) caught off the coast of Calp (Alicante, Spain) from 2019-11-01 to 2020-11-01. The samples were obtained from commercial bottom-trawl fisheries bycatch, carried out by local fishermen. For each sample, measurements were taken of the total length, weight, and gastro-intestinal tracts (GIT) weight. Individual plastic shapes such as fibers, fragments, films, and foams were identified and counted. This dataset contains the results from all 200 fish samples, in a spreadsheet format.

Dataset Citation

Cite as: Acevedo Quilis, Samuel; Hernández-Martínez, Anna Maria; Molera Arribas, Alejandro; Gámez Pérez, José; García-March, José Rafael; Tena Medialdea, José (2024). Microplastic concentrations in the digestive tracts of Scyliorhinus canicula (small-spotted catshark) caught off the coast of Calp (Alicante, Spain) from 2019-11-01 to 2020-11-01 (NCEI Accession 0292552). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0292552. Accessed [date].

Dataset Identifiers

ISO 19115-2 Metadata

gov.noaa.nodc:0292552

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Distribution Formats	Excel
Ordering Instructions	Contact NCEI for other distribution options and instructions.
Distributor	NOAA National Centers for Environmental Information +1-301-713-3277 NCEI.Info@noaa.gov
Dataset Point of Contact	NOAA National Centers for Environmental Information ncei.info@noaa.gov

Time Period	2019-11-01 to 2020-11-01
Spatial Bounding Box Coordinates	West: .068056 East: .533333 South: 38.217222 North: 38.952222
Spatial Coverage Map

General Documentation

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Descriptive Information
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Associated Resources

The NOAA NCEI Global Marine Microplastics Database (1972-present)
- NCEI Collection
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Quilis, S. A., Hernández-Martínez, A. M., Arribas, A. J. M., Pérez, J. G., García-March, J. R., & Medialdea, J. T. (2024). High prevalence of microplastics in the digestive tract of Scyliorhinus canicula (Linneaus, 1758) shows the species biomonitoring potential. Marine Pollution Bulletin. 2024 Jan 24; 200, 116051.
- https://doi.org/10.1016/j.marpolbul.2024.116051
  related journal article

gov.noaa.nodc:NCEI-Marine-Microplastics

Publication Dates	publication: 2024-05-13
Data Presentation Form	Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status	Complete - production of the data has been completed Historical archive - data has been stored in an offline storage facility
Data Update Frequency	As needed
Supplemental Information	Submission Package ID: TKM7FJ
Purpose	These microplastic concentration data were collected in order to determine their abundance in the digestive tracts of Scyliorhinus canicula (small-spotted catshark) caught off the coast of Calp (Alicante, Spain) from 2019-11-01 to 2020-11-01
Use Limitations	accessLevel: Public Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.

Dataset Citation	Cite as: Acevedo Quilis, Samuel; Hernández-Martínez, Anna Maria; Molera Arribas, Alejandro; Gámez Pérez, José; García-March, José Rafael; Tena Medialdea, José (2024). Microplastic concentrations in the digestive tracts of Scyliorhinus canicula (small-spotted catshark) caught off the coast of Calp (Alicante, Spain) from 2019-11-01 to 2020-11-01 (NCEI Accession 0292552). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0292552. Accessed [date].
Cited Authors	Acevedo Quilis, Samuel IMEDMAR-UCV Hernández-Martínez, Anna Maria IMEDMAR-UCV Molera Arribas, Alejandro IMEDMAR-UCV Gámez Pérez, José Universidad Jaume I García-March, José Rafael IMEDMAR-UCV Tena Medialdea, José IMEDMAR-UCV
Contributors	Catholic University of Valencia Saint Vincent Martyr (UCV)
Resource Providers	José Tena Medialdea Catholic University of Valencia Saint Vincent Martyr (UCV) Catholic University of Valencia Saint Vincent Martyr (UCV)
Points of Contact	Ebenezer Nyadjro Mississippi State University; Northern Gulf Institute (NGI)
Publishers	NOAA National Centers for Environmental Information
Acknowledgments	Related Funding Agency: European Union NextGenerationEU (# PRTR-C17.I1) Related Funding Agency: Generalitat Valenciana

Theme keywords	NODC DATA TYPES THESAURUS biological data INDIVIDUAL FISH EXAMINATION - LENGTH INDIVIDUAL FISH EXAMINATION - WEIGHT microplastic concentration SPECIES IDENTIFICATION NODC OBSERVATION TYPES THESAURUS in situ laboratory analyses WMO_CategoryCode oceanography Global Change Master Directory (GCMD) Science Keywords EARTH SCIENCE > BIOLOGICAL CLASSIFICATION EARTH SCIENCE > OCEANS > WATER QUALITY > SEA SURFACE CONTAMINANTS > MICROPLASTIC CONCENTRATION
Data Center keywords	NODC COLLECTING INSTITUTION NAMES THESAURUS Catholic University of Valencia Saint Vincent Martyr NODC SUBMITTING INSTITUTION NAMES THESAURUS Catholic University of Valencia Saint Vincent Martyr
Instrument keywords	NODC INSTRUMENT TYPES THESAURUS Fourier-transform infrared (FTIR) spectrometer microscope trawl Global Change Master Directory (GCMD) Instrument Keywords FTIR SPECTROMETER > Fourier Transform Infrared Spectrometer MICROSCOPES > MICROSCOPES TRAWL Provider Instruments bottom trawling
Place keywords	NODC SEA AREA NAMES THESAURUS Balearic (or Iberian) Sea Mediterranean Sea Mediterranean Sea - Western Basin Global Change Master Directory (GCMD) Location Keywords OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN > MEDITERRANEAN SEA Provider Place Names Atlantic Ocean
Keywords	NCEI ACCESSION NUMBER 0292552

Use Constraints	Cite as: Acevedo Quilis, Samuel; Hernández-Martínez, Anna Maria; Molera Arribas, Alejandro; Gámez Pérez, José; García-March, José Rafael; Tena Medialdea, José (2024). Microplastic concentrations in the digestive tracts of Scyliorhinus canicula (small-spotted catshark) caught off the coast of Calp (Alicante, Spain) from 2019-11-01 to 2020-11-01 (NCEI Accession 0292552). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0292552. Accessed [date].
Access Constraints	Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
Fees	In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.

Lineage information for: dataset
Processing Steps	2024-05-13T21:53:51Z - NCEI Accession 0292552 v1.1 was published.
Output Datasets	NCEI Accession 0292552 v1.1 NCEI Accession 0292552 v1.1 (download) published 2024-05-13T21:53:51Z

Lineage information for: dataset
Processing Steps	Parameter or Variable: microplastic concentration (measured); Units: count per individual; Observation Category: in situ; Sampling Instrument: bottom trawling; Sampling and Analyzing Method: The study was carried out off the coast of Calp (Alicante, Spain), in FAO area 37.1, Northern Spain, GSA-6 (Geographical subarea 6), established by the General Fisheries Commission for the Mediterranean (GFCM). A total of 200 female individuals of Scyliorhinus canicula were collected between November 2019 and November 2020, from commercial bottom-trawl fisheries bycatch, carried out by local fishermen. Biological parameters (total length (cm) and weight (g)) of the specimens were recorded. The dissection procedure was designed to reduce sample contamination. The extraction of the entire digestive tract was performed by fixation in the esophagus and rectum to prevent loss of stomach content. The gastrointestinal tracts (GITs) were weighed (g) and stored in properly labelled Petri dishes. To guarantee the preservation of the GITs samples, they were stored at -80◦C. In order to digest the GITs samples, a protocol adapted from Dehaut et al. (2016) was used. Extraction conditions were set at 60 ◦C for 24 h. at 300 rpm in 10 % KOH. Samples were then vacuum-filtered through a glass fiber filter disk (1.2 μm pore and 47 mm diameter, Whatman GF/CTM, Cytiva). To ensure that the procedure of extraction did not hinder or prevent the visual characterization of the MPs, it was validated in three replicates at 3 different temperatures (60 ◦C, 70 ◦C, 80 ◦C) and using manually-made plastic particles of 3 different sizes (1 mm, 2.5 mm, and 5 mm) of known polymers, such as polyethylene and high-density polyethylene (PE, HDPE), polystyrene (PS), polyethylene terephthalate (PET), polyamide-6 (PA6) and polycarbonate (PC). The filters obtained from the digestion of the 200 samples were observed under a stereoscopic microscope (Leica-MZ16). All isolated particles were counted, measured (in mm) and photographed. The identified plastics were classified according to their size (macro- >25 mm, meso- >5 mm, micro- <5 mm and nanoplastics <1 μm), shape (pellet, fiber, film, fragment and foam), color according to the protocol of the Marine Strategy Framework Directive (EMODnet). Chemical characterization of particles was carried out with a purification procedure of MPs samples, replicating the steps followed with H2O2 in an enzymatic protocol. The filters were submerged in 5 mL of H2O2 for 24 h prior to filtering. From the 200 total samples, 45 were purified and analyzed using Raman spectroscopy (Raman Apyron confocal microscope, Witec). Polymer identification was performed using the software Spectragryph v1.216.1. which allows the comparison of Raman profiles with other profiles obtained from a microplastics spectra database created by the Rochman Lab, University of Toronto.; Data Quality Method: Measures were taken to prevent contamination during the extraction process. Cotton laboratory coats and nitrile gloves were used. Glassware was thoroughly washed and rinsed with deionized water before use, and flasks were heated to remove grubbiness. Filters were kept in labelled Petri dishes. As a control, to avoid air contamination, filter in Petri dishes were exposed to laboratory air during the selection process. In the presence of obvious external contamination, the corresponding samples were discarded..

Lineage information for: dataset

Processing Steps

Parameter or Variable: microplastic concentration (measured); Units: count per individual; Observation Category: in situ; Sampling Instrument: bottom trawling; Sampling and Analyzing Method: The study was carried out off the coast of Calp (Alicante, Spain), in FAO area 37.1, Northern Spain, GSA-6 (Geographical subarea 6), established by the General Fisheries Commission for the Mediterranean (GFCM). A total of 200 female individuals of Scyliorhinus canicula were collected between November 2019 and November 2020, from commercial bottom-trawl fisheries bycatch, carried out by local fishermen. Biological parameters (total length (cm) and weight (g)) of the specimens were recorded. The dissection procedure was designed to reduce sample contamination. The extraction of the entire digestive tract was performed by fixation in the esophagus and rectum to prevent loss of stomach content. The gastrointestinal tracts (GITs) were weighed (g) and stored in properly labelled Petri dishes. To guarantee the preservation of the GITs samples, they were stored at -80◦C. In order to digest the GITs samples, a protocol adapted from Dehaut et al. (2016) was used. Extraction conditions were set at 60 ◦C for 24 h. at 300 rpm in 10 % KOH. Samples were then vacuum-filtered through a glass fiber filter disk (1.2 μm pore and 47 mm diameter, Whatman GF/CTM, Cytiva). To ensure that the procedure of extraction did not hinder or prevent the visual characterization of the MPs, it was validated in three replicates at 3 different temperatures (60 ◦C, 70 ◦C, 80 ◦C) and using manually-made plastic particles of 3 different sizes (1 mm, 2.5 mm, and 5 mm) of known polymers, such as polyethylene and high-density polyethylene (PE, HDPE), polystyrene (PS), polyethylene terephthalate (PET), polyamide-6 (PA6) and polycarbonate (PC). The filters obtained from the digestion of the 200 samples were observed under a stereoscopic microscope (Leica-MZ16). All isolated particles were counted, measured (in mm) and photographed. The identified plastics were classified according to their size (macro- >25 mm, meso- >5 mm, micro- <5 mm and nanoplastics <1 μm), shape (pellet, fiber, film, fragment and foam), color according to the protocol of the Marine Strategy Framework Directive (EMODnet). Chemical characterization of particles was carried out with a purification procedure of MPs samples, replicating the steps followed with H2O2 in an enzymatic protocol. The filters were submerged in 5 mL of H2O2 for 24 h prior to filtering. From the 200 total samples, 45 were purified and analyzed using Raman spectroscopy (Raman Apyron confocal microscope, Witec). Polymer identification was performed using the software Spectragryph v1.216.1. which allows the comparison of Raman profiles with other profiles obtained from a microplastics spectra database created by the Rochman Lab, University of Toronto.; Data Quality Method: Measures were taken to prevent contamination during the extraction process. Cotton laboratory coats and nitrile gloves were used. Glassware was thoroughly washed and rinsed with deionized water before use, and flasks were heated to remove grubbiness. Filters were kept in labelled Petri dishes. As a control, to avoid air contamination, filter in Petri dishes were exposed to laboratory air during the selection process. In the presence of obvious external contamination, the corresponding samples were discarded..

Acquisition Information (collection)
Instrument	Fourier-transform infrared (FTIR) spectrometer microscope trawl

Last Modified: 2024-05-14T17:55:08Z
For questions about the information on this page, please email: ncei.info@noaa.gov

Microplastic concentrations in the digestive tracts of Scyliorhinus canicula (small-spotted catshark) caught off the coast of Calp (Alicante, Spain) from 2019-11-01 to 2020-11-01 (NCEI Accession 0292552)

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