Skip to main content
Dataset Overview | National Centers for Environmental Information (NCEI)

Diel proteomes of cultured Trichodesmium erythraeum sp. IMS101 from laboratory experiments conducted in November of 2018 (NCEI Accession 0292202)

browse graphicGraphic not available.
This dataset contains biological and chemical data collected on 2018-11-01. These data include proteins. The instruments used to collect these data include Mass Spectrometer. These data were collected by Mak A. Saito of Woods Hole Oceanographic Institution as part of the "Marine Microbial Investigator Award: Investigator Mak Saito (MM Saito)" and "New technology for high resolution analysis of proteins and other organic materials produced by marine microorganisms (MM Proteins and Organics Tech)" projects. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2020-03-25.

The following is the text of the dataset description provided by BCO-DMO:

Dataset Description: Diel proteomes of cultured Trichodesmium erythraeum sp. IMS101 from laboratory experiments conducted in November of 2018.

The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE [1] partner repository with the dataset identifier PXD016332 and 10.6019/PXD016332 but are not yet public.

Project Name: Trichodesmium erythraeum sp. IMS101 Diel proteomes

Project accession: PXD016332

Project https://doi.org/10.6019/PXD016332

The format of these data in the BCO-DMO data system is tabular. For a version formatted as a matrix, see the "Data Files" section.
  • Cite as: Saito, Mak A. (2024). Diel proteomes of cultured Trichodesmium erythraeum sp. IMS101 from laboratory experiments conducted in November of 2018 (NCEI Accession 0292202). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0292202. Accessed [date].
gov.noaa.nodc:0292202
Full Text · XML
Download Data
  • HTTPS (download)
    Navigate directly to the URL for data access and direct download.
  • FTP (download)
    These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP).
Distribution Formats
  • TSV
Ordering Instructions Contact NCEI for other distribution options and instructions.
Distributor NOAA National Centers for Environmental Information
+1-301-713-3277
NCEI.Info@noaa.gov
Dataset Point of Contact NOAA National Centers for Environmental Information
ncei.info@noaa.gov
Time Period 2018-11-01 to 2018-11-01
Spatial Bounding Box Coordinates
West:
East:
South:
North:
Spatial Coverage Map
General Documentation
Associated Resources
  • Biological, chemical, physical, biogeochemical, ecological, environmental and other data collected from around the world during historical and contemporary periods of biological and chemical oceanographic exploration and research managed and submitted by the Biological and Chemical Oceanography Data Management Office (BCO-DMO)
    • NCEI Collection
      Navigate directly to the URL for data access and direct download.
  • Held, N., Saito, M. A. (2019) Diel proteomes of cultured Trichodesmium erythraeum sp. IMS101 from laboratory experiments conducted in November of 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). Dataset version 2019-12-10. https://doi.org/10.26008/1912/bco-dmo.783873.1
    • Parent ID (indicates this dataset is related to other data):
    • gov.noaa.nodc:BCO-DMO
Publication Dates
  • publication: 2024-05-02
Data Presentation Form Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status Complete - production of the data has been completed
Historical archive - data has been stored in an offline storage facility
Data Update Frequency As needed
Supplemental Information
Acquisition Description:
A batch culture (1.5L) of Trichodesmium erythraeum sp. IMS101 was grown in a 27°C incubator with a 14:10 light cycle that ramps up and down mimicking dawn and dusk. Sampling occurred every 1-3 hours with concentrated sampling at dawn and dusk. 70mL of the culture was sterically subsampled and collected on 0.2mm Supor filters, then frozen at -80°C. 10mL was collected and filtered on combusted GFF filters for CHN analysis.

Proteins were extracted in sodium dodecyl sulfate and digested in gel similar to Saito et al., 2014 (Science). Peptides were analyzed by LC-MS/MS on a Thermo Orbitrap Fusion using LC x LC/MS chromatography with high and low pH reversed phase chromatography.
Purpose This dataset is available to the public for a wide variety of uses including scientific research and analysis.
Use Limitations
  • accessLevel: Public
  • Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.
Dataset Citation
  • Cite as: Saito, Mak A. (2024). Diel proteomes of cultured Trichodesmium erythraeum sp. IMS101 from laboratory experiments conducted in November of 2018 (NCEI Accession 0292202). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0292202. Accessed [date].
Cited Authors
Principal Investigators
Contributors
Resource Providers
Points of Contact
Publishers
Acknowledgments
Theme keywords NODC DATA TYPES THESAURUS NODC OBSERVATION TYPES THESAURUS WMO_CategoryCode
  • oceanography
BCO-DMO Standard Parameters Global Change Master Directory (GCMD) Science Keywords Originator Parameter Names
Data Center keywords NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS Global Change Master Directory (GCMD) Data Center Keywords
Instrument keywords NODC INSTRUMENT TYPES THESAURUS BCO-DMO Standard Instruments Global Change Master Directory (GCMD) Instrument Keywords Originator Instrument Names
Project keywords BCO-DMO Standard Projects Provider Funding Award Information
Keywords NCEI ACCESSION NUMBER
Use Constraints
  • Cite as: Saito, Mak A. (2024). Diel proteomes of cultured Trichodesmium erythraeum sp. IMS101 from laboratory experiments conducted in November of 2018 (NCEI Accession 0292202). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0292202. Accessed [date].
Data License
Access Constraints
  • Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
Fees
  • In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.
Lineage information for: dataset
Processing Steps
  • 2024-05-02T16:07:12Z - NCEI Accession 0292202 v1.1 was published.
Output Datasets
Acquisition Information (collection)
Instrument
  • mass spectrometer
Last Modified: 2024-06-04T18:34:55Z
For questions about the information on this page, please email: ncei.info@noaa.gov