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Microbial enzyme activities: peptidase activities of sediment samples from the RV\Polarstern cruise ARKXXVII/3 in the Central Arctic Ocean and Laptev Sea, Aug-Sept. 2012 (NCEI Accession 0292005)

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This dataset contains data collected on R/V Polarstern during cruise ARK-XXVII-3 from 2012-08-09 to 2012-09-22. These data include depth. The instruments used to collect these data include CTD profiler, Centrifuge, Fluorometer, and Multi Corer. These data were collected by Carol Arnosti of University of North Carolina at Chapel Hill as part of the "Latitudinal and depth-related contrasts in enzymatic capabilities of pelagic microbial communities: Predictable patterns in the ocean? (Patterns of activities)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2020-04-27.

The following is the text of the dataset description provided by BCO-DMO:

ARK27-3: sediment MCA

Dataset Description:
This dataset includes peptidase hydrolysis rates from sediments to measure microbial enzyme activities. Links to archived CTD data are also provided. Five substrates linked to a 7-amido-4-methyl coumarin (MCA) fluorophore, one amino acid – leucine – and four oligopeptides – the chymotrypsin substrates alanine-alanine-phenylalanine (AAF) and alanine-alanine-proline-phenylalanine (AAPF), and the trypsin substrates glutamine-alanine-arginine (QAR) and glutamic acid-gylcine-arginine (EGR) – were used to measure exo- and endo-acting peptidase activities, respectively.

Dataset Citation

Cite as: Arnosti, Carol (2024). Microbial enzyme activities: peptidase activities of sediment samples from the RV\Polarstern cruise ARKXXVII/3 in the Central Arctic Ocean and Laptev Sea, Aug-Sept. 2012 (NCEI Accession 0292005). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0292005. Accessed [date].

Dataset Identifiers

ISO 19115-2 Metadata

gov.noaa.nodc:0292005

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Download Data	HTTPS (download) Navigate directly to the URL for data access and direct download. FTP (download) These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP).
Distribution Formats	TSV
Ordering Instructions	Contact NCEI for other distribution options and instructions.
Distributor	NOAA National Centers for Environmental Information +1-301-713-3277 NCEI.Info@noaa.gov
Dataset Point of Contact	NOAA National Centers for Environmental Information ncei.info@noaa.gov

Time Period	2012-08-09 to 2012-09-22
Spatial Bounding Box Coordinates	West: 31.21 East: 130.5795 South: 79.6502 North: 88.809
Spatial Coverage Map

General Documentation

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Descriptive Information
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Associated Resources

Biological, chemical, physical, biogeochemical, ecological, environmental and other data collected from around the world during historical and contemporary periods of biological and chemical oceanographic exploration and research managed and submitted by the Biological and Chemical Oceanography Data Management Office (BCO-DMO)
- NCEI Collection
  Navigate directly to the URL for data access and direct download.
peptidase activities of sediment samples from the RV\Polarstern cruise ARKXXVII/3 in the Central Arctic Ocean and Laptev Sea, Aug-Sept. 2012. Dataset version 2018-07-24. https://doi.org/10.26008/1912/bco-dmo.743018.1
- https://doi.org/10.26008/1912/bco-dmo.743018.1 (download)
  originator dataset

gov.noaa.nodc:BCO-DMO

Publication Dates	publication: 2024-04-29
Data Presentation Form	Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status	Complete - production of the data has been completed Historical archive - data has been stored in an offline storage facility
Data Update Frequency	As needed
Supplemental Information	Acquisition Description: Surficial sediments were collected using a multi-corer. Five substrates linked to a 7-amido-4-methyl coumarin (MCA) fluorophore, one amino acid – leucine – and four oligopeptides – the chymotrypsin substrates alanine-alanine-phenylalanine (AAF) and alanine-alanine-proline-phenylalanine (AAPF), and the trypsin substrates glutamine-alanine-arginine (QAR) and glutamic acid-gylcine-arginine (EGR) – were used to measure exo- and endo-acting peptidase activities, respectively. Hydrolysis rates of the substrates were measured as an increase in fluorescence as the fluorophore was hydrolyzed from the substrate over time [as in Hoppe, 1993; Obayashi and Suzuki, 2005]. For sediment measurements, peptidase activities were measured in a 1:2 (vol: vol) sediment: autoclaved seawater slurry. 100 micromolar concentrations of substrate were added to triplicate live and single autoclaved killed control incubations. 2 ml of slurry was centrifuged at each timepoint (0, 1h ,2h, and 4 h), filtered, and diluted with 1 ml borate buffer; fluorescence was measured in a 4 ml cuvette. Hydrolysis rates were calculated from the rate of increase of fluorescence in the incubation over time relative to a set of standards of known concentration of fluorophore. All incubations were conducted at 0 C in the dark.
Purpose	This dataset is available to the public for a wide variety of uses including scientific research and analysis.
Use Limitations	accessLevel: Public Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.

Dataset Citation	Cite as: Arnosti, Carol (2024). Microbial enzyme activities: peptidase activities of sediment samples from the RV\Polarstern cruise ARKXXVII/3 in the Central Arctic Ocean and Laptev Sea, Aug-Sept. 2012 (NCEI Accession 0292005). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0292005. Accessed [date].
Cited Authors	Arnosti, Carol University of North Carolina at Chapel Hill
Principal Investigators	Carol Arnosti University of North Carolina - Chapel Hill (UNC)
Contributors	University of North Carolina - Chapel Hill (UNC)
Resource Providers	Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Points of Contact	Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Publishers	NOAA National Centers for Environmental Information
Acknowledgments	Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1332881 Award URL: http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1332881

Theme keywords	NODC DATA TYPES THESAURUS DEPTH - OBSERVATION WMO_CategoryCode oceanography BCO-DMO Standard Parameters ISO_DateTime_UTC cast depth external_link latitude longitude no standard parameter station time_elapsed time_point Originator Parameter Names ISO_DateTime_UTC Latitude Longitude average cast_no comments depth_m depth_no rep1_rate rep2_rate rep3_rate station_no std_dev substrate time_elapsed_hr timepoint
Data Center keywords	NODC COLLECTING INSTITUTION NAMES THESAURUS University of North Carolina - Chapel Hill NODC SUBMITTING INSTITUTION NAMES THESAURUS Biological and Chemical Oceanography Data Management Office Global Change Master Directory (GCMD) Data Center Keywords BCO-DMO > Biological and Chemical Oceanography Data Management Office
Platform keywords	NODC PLATFORM NAMES THESAURUS RV Polarstern BCO-DMO Platform Names R/V Polarstern Global Change Master Directory (GCMD) Platform Keywords Ships ICES/SeaDataNet Ship Codes Polarstern (call sign: DBLK, ICES code: 06AQ, 1982)
Instrument keywords	NODC INSTRUMENT TYPES THESAURUS CTD fluorometer sediment sampler - corer BCO-DMO Standard Instruments CTD profiler Centrifuge Fluorometer Multi Corer Global Change Master Directory (GCMD) Instrument Keywords CTD > Conductivity, Temperature, Depth FLUOROMETERS > FLUOROMETERS SEDIMENT CORERS > SEDIMENT CORERS Originator Instrument Names mini fluorometer unknown (CTD profiler) unknown (Centrifuge) unknown (Multi Corer)
Place keywords	Provider Place Names Central Arctic Ocean, Laptev Sea
Project keywords	BCO-DMO Standard Projects Latitudinal and depth-related contrasts in enzymatic capabilities of pelagic microbial communities: Predictable patterns in the ocean? (Patterns of activities) Provider Cruise IDs ARK-XXVII-3 Provider Funding Award Information Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1332881
Keywords	NCEI ACCESSION NUMBER 0292005

Use Constraints	Cite as: Arnosti, Carol (2024). Microbial enzyme activities: peptidase activities of sediment samples from the RV\Polarstern cruise ARKXXVII/3 in the Central Arctic Ocean and Laptev Sea, Aug-Sept. 2012 (NCEI Accession 0292005). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0292005. Accessed [date].
Data License	This dataset is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License. SPDX License: Creative Commons Attribution 4.0 International (CC-BY-4.0)
Access Constraints	Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
Fees	In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.

Lineage information for: dataset
Processing Steps	2024-04-29T15:40:24Z - NCEI Accession 0292005 v1.1 was published.
Output Datasets	NCEI Accession 0292005 v1.1 NCEI Accession 0292005 v1.1 (download) published 2024-04-29T15:40:24Z

Acquisition Information (collection)
Instrument	CTD fluorometer sediment sampler - corer
Platform	RV Polarstern

Last Modified: 2024-05-31T15:15:28Z
For questions about the information on this page, please email: ncei.info@noaa.gov

Microbial enzyme activities: peptidase activities of sediment samples from the RV\Polarstern cruise ARKXXVII/3 in the Central Arctic Ocean and Laptev Sea, Aug-Sept. 2012 (NCEI Accession 0292005)

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