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Carbonate chemistry over a time course with Ulva in pH drift experiments (Seaweed OA Resilience project) from 2015-05-11 to 2015-07-27 (NCEI Accession 0291979)

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This dataset contains chemical and physical data collected from 2015-05-11 to 2015-07-27. These data include Carbonate concentration [CO3]2-, bicarbonate concentration [HCO3]-, dissolved inorganic Carbon, pCO2, pH, salinity calculated from CTD primary sensors, total Carbon Dioxide in seawater, total alkalinity, and water temperature. The instruments used to collect these data include Aquarium chiller, Automatic titrator, Mass Flow Controller, Multi Parameter Bench Meter, and UV Spectrophotometer-Shimadzu. These data were collected by Dr Janet E Kubler and Dr Steve Dudgeon of California State University Northridge as part of the "Ocean Acidification: Scope for Resilience to Ocean Acidification in Macroalgae (Seaweed OA Resilience)" project and "Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA) (SEES-OA)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-06-03.

The following is the text of the dataset description provided by BCO-DMO:

Carbonate Chemistry over a time course with Ulva in pH drift experiments

Dataset Description:
Seawater samples were collected from a pH drift experiment with Ulva lactuca to test the mechanism of carbon acquisition by the alga and the effect on carbonate chemistry parameters reported in these datasets. This dataset reports the pH, dissolved inorganic carbon (DIC), carbon diaoxide (CO2), bicarbonate and carbonate concentrations of seawater at initial and 24 hour times from pH drift experiment that used Ulva lactuca grown in closed culture pots at varying pCO2 levels.

Related Datasets:
: Carbonate chemistry of Ulva lactuca culture pots testing the effects of pCO2 variability (Seaweed OA Resilience project)
: Chlorophyll a per unit biomass in Ulva lactuca under ocean acidification (OA) conditions (Seaweed OA Resilience project)
: Stable isotope ratios and mass of carbon and nitrogen in Ulva cells under ocean acidification conditions (Seaweed OA Resilience project)
: Growth rates of Ulva exposed to different average and variability of pCO2 (Seaweed OA Resilience project)
: Time-series at 10 minute sampling interval of pH and temperature in Ulva culture pots (Seaweed OA Resilience project)
: Rates of photosynthesis and respiration by Ulva exposed to different average and variability of pCO2 (Seaweed OA Resilience project)
: Stable isotope ratio and concentration of carbon in seawater from Ulva OA experiments (Seaweed OA Resilience project)
  • Cite as: Kubler, Janet E.; Dudgeon, Steve (2024). Carbonate chemistry over a time course with Ulva in pH drift experiments (Seaweed OA Resilience project) from 2015-05-11 to 2015-07-27 (NCEI Accession 0291979). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0291979. Accessed [date].
gov.noaa.nodc:0291979
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Distributor NOAA National Centers for Environmental Information
+1-301-713-3277
NCEI.Info@noaa.gov
Dataset Point of Contact NOAA National Centers for Environmental Information
ncei.info@noaa.gov
Time Period 2015-05-11 to 2015-07-27
Spatial Bounding Box Coordinates
West: -119
East: -118
South: 33
North: 34
Spatial Coverage Map
General Documentation
Associated Resources
  • Biological, chemical, physical, biogeochemical, ecological, environmental and other data collected from around the world during historical and contemporary periods of biological and chemical oceanographic exploration and research managed and submitted by the Biological and Chemical Oceanography Data Management Office (BCO-DMO)
    • NCEI Collection
      Navigate directly to the URL for data access and direct download.
  • Kubler, J., Dudgeon, S. (2018) Carbonate chemistry over a time course with Ulva in pH drift experiments (Seaweed OA Resilience project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Dataset version 2018-03-22. https://doi.org/10.1575/1912/bco-dmo.732464.1
    • Parent ID (indicates this dataset is related to other data):
    • gov.noaa.nodc:BCO-DMO
Publication Dates
  • publication: 2024-04-29
Data Presentation Form Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status Complete - production of the data has been completed
Historical archive - data has been stored in an offline storage facility
Data Update Frequency As needed
Supplemental Information
Acquisition Description:
Samples were collected at 0 (initial) and 24 hours and parameters of carbonate chemistry were measured (DIC,CO2, HCO3,CO3-described below). pH drift assay experiments consisted of 2 independent variables; inhibitor treatment and algal presence, with 4 and 2 levels, respectively for each independent variable. The inhibitor treatments included: seawater only (control), Acetazolamide (A), 4,4’-Diisothiocyano-2,2’-stilbenedisulfonic acid (DIDS), and Ethoxyzolamide (E). Algae were either present or absent (control) in a vial. Two replicate water samples were collected from each of 10 experimental culture pots of algae grown for 3 weeks at different combinations of average and standard deviations of pCO2 level during each of 3 experimental trials and assayed after 24 hours with or without the presence of Ulva and combinations of inhibitor treatments. In addition, 3 different levels of pCO2 in seawater generated independently of the seawater in algal culture that represented the range of pCO2 levels assayed across the entire experiment were assayed over the same 24 hour period.

Carbonate chemistry parameters were measured by sampling pH and total alkalinity (TA) of water samples. pH was determined using the m-cresol indicator dye method in a spectrophotometer (Dickson et al. 2007). TA samples were analyzed by potentiometric titration coupled to a pH electrode calibrated using certified reference material (CRM) from the Dickson laboratory at Scripps Oceanographic Institute and the pH electrode calibrated using TRIS buffer (Dickson et al. 2007). TA and carbonate parameters were calculated from potentiometric titration data and spectrophotometric pH data.
Purpose This dataset is available to the public for a wide variety of uses including scientific research and analysis.
Use Limitations
  • accessLevel: Public
  • Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.
Dataset Citation
  • Cite as: Kubler, Janet E.; Dudgeon, Steve (2024). Carbonate chemistry over a time course with Ulva in pH drift experiments (Seaweed OA Resilience project) from 2015-05-11 to 2015-07-27 (NCEI Accession 0291979). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0291979. Accessed [date].
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Theme keywords NODC DATA TYPES THESAURUS NODC OBSERVATION TYPES THESAURUS WMO_CategoryCode
  • oceanography
BCO-DMO Standard Parameters Global Change Master Directory (GCMD) Science Keywords Originator Parameter Names
Data Center keywords NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS Global Change Master Directory (GCMD) Data Center Keywords
Instrument keywords NODC INSTRUMENT TYPES THESAURUS BCO-DMO Standard Instruments Global Change Master Directory (GCMD) Instrument Keywords Originator Instrument Names
Project keywords BCO-DMO Standard Programs BCO-DMO Standard Projects Provider Funding Award Information
Keywords NCEI ACCESSION NUMBER
Use Constraints
  • Cite as: Kubler, Janet E.; Dudgeon, Steve (2024). Carbonate chemistry over a time course with Ulva in pH drift experiments (Seaweed OA Resilience project) from 2015-05-11 to 2015-07-27 (NCEI Accession 0291979). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0291979. Accessed [date].
Data License
Access Constraints
  • Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
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  • In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.
Lineage information for: dataset
Processing Steps
  • 2024-04-29T15:25:12Z - NCEI Accession 0291979 v1.1 was published.
Output Datasets
Acquisition Information (collection)
Instrument
  • flow meter
  • spectrophotometer
  • titrator
Last Modified: 2024-05-31T18:50:46Z
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