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Dataset Overview | National Centers for Environmental Information (NCEI)

Microsatellite genotypes of Breviolum sp. symbionts within Orbicella faveolata recruits outplanted to reefs in the Florida Keys from 2009-2011 (SymBioSys project) (NCEI Accession 0291950)

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This dataset contains data collected from 2009-01-01 to 2011-12-31. These data include depth. These data were collected by David Sheets of Canisius College, Margaret W Miller of NOAA Southeast Fisheries Science Center, and Mary Alice Coffroth of University at Buffalo as part of the "Ontogenic change in Cnidarian-algal symbioses: A genomic and ecologic perspective (SymBioSys)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2023-05-24.

The following is the text of the dataset description provided by BCO-DMO:

Acquisition Description:
Sampling and Analytical Methodology:

Egg-sperm bundles were collected from adult colonies of O. faveolata following the techniques described by Miller (2014). Larvae were reared at a shore-based lab and settled onto terracotta tiles . Once the larvae had metamorphosed and attached to the tiles, they were outplanted onto the reef by attachment vertically to a PVC rack approximately 0.2 m above the substrate to allow uptake of algal symbionts. Tiles with recruits were sampled one to three months after outplanting, recruits removed from the tile and preserved individually in 95% ethanol for subsequent molecular analysis. DNA extraction followed Coffroth et al. (1992). Genotypes of the algal symbionts within the genus Breviolum were characterized using three polymorphic microsatellite loci, B7Sym34, B7Sym36 and CA6.38, which were adapted for use with O. faveolata (Thornhill et al. 2009).

Tissue samples were collected from the top, middle and bottom portions of adult Orbicella faveolata colonies using the syringe method as described in Correa et al. (2009) and DNA extracted following Coffroth et al. (1992). Cp-23S genotypes of the algal symbionts within the genus Breviolum were characterized following the protocol of Santos et al. (2003).

Locations in the Florida Keys with Abbreviations

Alligator (AR) Coral Garden (CG) Cheeca Rocks (CR) East Turtle (ET) Grecian Rocks (GR) Looe Key (LK) Sand Island (SI) Tennessee (TR)
  • Cite as: Coffroth, Mary Alice; Miller, Margaret W.; Sheets, David (2024). Microsatellite genotypes of Breviolum sp. symbionts within Orbicella faveolata recruits outplanted to reefs in the Florida Keys from 2009-2011 (SymBioSys project) (NCEI Accession 0291950). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0291950. Accessed [date].
gov.noaa.nodc:0291950
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Distributor NOAA National Centers for Environmental Information
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Dataset Point of Contact NOAA National Centers for Environmental Information
ncei.info@noaa.gov
Time Period 2009-01-01 to 2011-12-31
Spatial Bounding Box Coordinates
West: -81.4094
East: -80.2833
South: 24.5449
North: 25.1167
Spatial Coverage Map
General Documentation
Associated Resources
  • Biological, chemical, physical, biogeochemical, ecological, environmental and other data collected from around the world during historical and contemporary periods of biological and chemical oceanographic exploration and research managed and submitted by the Biological and Chemical Oceanography Data Management Office (BCO-DMO)
    • NCEI Collection
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  • Coffroth, M. A., Miller, M. W., Sheets, D. (2023) Microsatellite genotypes of Breviolum sp. symbionts within Orbicella faveolata recruits outplanted to reefs in the Florida Keys from 2009-2011 (SymBioSys project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-03-07. https://doi.org/10.26008/1912/bco-dmo.887265.1
    • Parent ID (indicates this dataset is related to other data):
    • gov.noaa.nodc:BCO-DMO
Publication Dates
  • publication: 2024-04-27
Data Presentation Form Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status Complete - production of the data has been completed
Historical archive - data has been stored in an offline storage facility
Data Update Frequency As needed
Purpose This dataset is available to the public for a wide variety of uses including scientific research and analysis.
Use Limitations
  • accessLevel: Public
  • Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.
Dataset Citation
  • Cite as: Coffroth, Mary Alice; Miller, Margaret W.; Sheets, David (2024). Microsatellite genotypes of Breviolum sp. symbionts within Orbicella faveolata recruits outplanted to reefs in the Florida Keys from 2009-2011 (SymBioSys project) (NCEI Accession 0291950). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0291950. Accessed [date].
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Acknowledgments
Use Constraints
  • Cite as: Coffroth, Mary Alice; Miller, Margaret W.; Sheets, David (2024). Microsatellite genotypes of Breviolum sp. symbionts within Orbicella faveolata recruits outplanted to reefs in the Florida Keys from 2009-2011 (SymBioSys project) (NCEI Accession 0291950). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0291950. Accessed [date].
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Lineage information for: dataset
Processing Steps
  • 2024-04-27T17:38:24Z - NCEI Accession 0291950 v1.1 was published.
Output Datasets
Last Modified: 2024-05-31T18:50:46Z
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