Series 1B-3: Multiple stressor experiments on T. pseudonana (CCMP1335) – computed data from the LC3 protocol for samples at 4 temperatures, 15-26C from 2018-11-17 to 2019-04-16 (NCEI Accession 0291933)
This dataset contains biological, optical, and physical data collected from 2018-11-17 to 2019-04-16. These data include Fv to Fm ratio, fluorescence, and water temperature. The instruments used to collect these data include Cell Cultivator, Fluorometer, and Spectrophotometer. These data were collected by Edward Laws of Louisiana State University and Julia Sweet and Uta Passow of University of California-Santa Barbara as part of the "Collaborative Research: Effects of multiple stressors on Marine Phytoplankton (Stressors on Marine Phytoplankton)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2020-11-30.
The following is the text of the dataset description provided by BCO-DMO:
Series 1B: photophysiology
Dataset Description:
The raw fluorescence data can be found under the Data Files section as an Excel file and as individual .csv files.
The following is the text of the dataset description provided by BCO-DMO:
Series 1B: photophysiology
Dataset Description:
The raw fluorescence data can be found under the Data Files section as an Excel file and as individual .csv files.
Dataset Citation
- Cite as: Passow, Uta; Laws, Edward; Sweet, Julia (2024). Series 1B-3: Multiple stressor experiments on T. pseudonana (CCMP1335) – computed data from the LC3 protocol for samples at 4 temperatures, 15-26C from 2018-11-17 to 2019-04-16 (NCEI Accession 0291933). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0291933. Accessed [date].
Dataset Identifiers
ISO 19115-2 Metadata
gov.noaa.nodc:0291933
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Ordering Instructions | Contact NCEI for other distribution options and instructions. |
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NOAA National Centers for Environmental Information +1-301-713-3277 NCEI.Info@noaa.gov |
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NOAA National Centers for Environmental Information ncei.info@noaa.gov |
Time Period | 2018-11-17 to 2019-04-16 |
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Data Presentation Form | Digital table - digital representation of facts or figures systematically displayed, especially in columns |
Dataset Progress Status | Complete - production of the data has been completed Historical archive - data has been stored in an offline storage facility |
Data Update Frequency | As needed |
Supplemental Information | Acquisition Description: Experimental setup: The experiments were designed to test the combined effects of four temperatures, and eight light intensities on growth and photophysiology of the diatom T. pseudonana CCMP1335 in a multifactorial design. Four temperatures were tested: 15°C, 18°C, 22°C, and 26°C. Within each temperature, eight light levels were tested: 30, 40, 70,90,105,125,140 and 265 µmol photons · m-2 · s-1. All lights were set at a 12 h day: 12 h dark cycle. For logistical reasons, experiments were partially conducted in series. Experiments were conducted in Multicultivator MC-1000 OD units (Photon Systems Instruments, Drasov, Czech Republic). Each unit consists of eight 85 ml test-tubes immersed in a thermostated water bath, each independently illuminated by an array of cool white LEDs set at specific intensity and timing. A 0.2µm filtered ambient air was bubbled through sterile artificial seawater, and the humidified air was supplied to each tube Each experiment was split into two phases: An acclimation phase spanning 3 days, was used to acclimate cultures to their new environment. Pre-acclimated, exponentially-growing cultures were then inoculated into fresh media and incubated through a 4-day experimental phase during which assessments of growth, photophysiology, and nutrient cycling were carried out daily. All sampling started 6 hours into the daily light cycle to minimize the effects of diurnal cycles. Experiments were conducted with artificial seawater (ASW) prepared using previously described methods (Kester et. al 1967), and enriched with 50mL per liter of UV sterilized natural seawater and nitrate (NO3), phosphate (PO4), silicic acid (Si[OH]4), at levels ensuring that the cultures would remain nutrient-replete over the course of the experiment. Trace metals and vitamins were added as in f/2 (Guillard 1975). The pH of the growth media was measured spectrophotometrically using the m-cresol purple method (Dickson 1993), and adjusted using 0.1N HCl or 0.1M NaOH. Photophysiology Photophysiology was assessed daily using a handheld Pulse Amplitude Modulated (PAM) fluorometer (AquaPen-C AP-C 100, Photon System Instruments, Czech Republic). A sample was collected from each light treatment for each, 5 hours after the start of the daily light cycle, and placed in the dark for a minimum of 30 minutes prior to measurements. The dark-adapted sample was used to generate light curves that provide measurements of in-vivo chlorophyll autofluorescence (F0), the maximum quantum yield (QYmax or Fv/Fm), and relative photosynthesis rates based on PSII quantum yields at varying light intensities - using the instrument’s LC3 protocol. The LC3 protocol involves measurements of baseline and maximal fluorescence over seven 60-second phases, with each phase representing a light intensity from 10 to 1000 μmol photons m-2 · s-1. Blue light (455 nm) was used as actinic light in these experiments, and measurements were made at measuring illumination (f-pulse) intensity of 0.03 μmol photons m-2 · s-1, and saturating (F-pulse) illumination of 2100 μmol photons m-2 · s-1, and actinic illumination (A-pulse) controlled by the instrument’s protocol were set at 10, 20, 50, 100, 300, 500, and 1000 μmol photons m-2 · s-1 (for each 60-second phase). |
Purpose | This dataset is available to the public for a wide variety of uses including scientific research and analysis. |
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Last Modified: 2024-05-31T15:15:28Z
For questions about the information on this page, please email: ncei.info@noaa.gov
For questions about the information on this page, please email: ncei.info@noaa.gov