Symbiont community structure in Orbicella faveolata from Mermaid Reef and Sandy Cay Reef in Abaco, The Bahamas in January 2019 (NCEI Accession 0291746)

This dataset contains biological and survey - biological data collected on 2019-01-24. These data include depth, genus, and species. The instruments used to collect these data include qPCR Thermal Cycler. These data were collected by Katie Parker and Ross Cunning of Shedd Aquarium as part of the "Collaborative Research: Assessing the changing symbiotic milieu on Caribbean coral reefs under climate change: magnitude, tradeoffs, interventions, and implications (Symbiont Shifts on Reefs)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2021-11-15.

The following is the text of the dataset description provided by BCO-DMO:

Symbiont community structure in Orbicella faveolata

Dataset Description:
Acquisition Description:
Tissue samples from colonies of O. faveolata were collected from Mermaid Reef and sensitive Sandy Cay Reef between 21 and 24 January 2019. Genomic DNA from each coral sample was extracted and assayed using qPCR targeting loci specific to O. faveolata and each of four symbiont genera ( Symbiodinium, Breviolum, Cladocopium, and Durusdinium ). All reactions were performed on a QuantStudio 3 Real-Time PCR system (Life Technologies, Foster City, CA, USA). The resulting cycle threshold (CT) values at a set threshold of delta Rn = 0.01 were used for data analysis.