Incubation experiments were conducted in St. John, US Virgin Islands to investigate the response of reef seawater microbial communities to the mixed exudates released from the coral species Porites astreoides and Gorgonia ventalina on 2022-11-10 (NCEI Accession 0291732)
This dataset contains biological and survey - biological data collected on 2022-11-10. These data include prochlorococcus abundance and synechococcus abundance. The instruments used to collect these data include Automated DNA Sequencer and Shimadzu TOC-V Analyzer. These data were collected by Amy Apprill, Elizabeth Kujawinski, and Laura Weber of Woods Hole Oceanographic Institution as part of the "Signature exometabolomes of Caribbean corals and influences on reef picoplankton (Coral Exometabolomes)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2022-11-10.
The following is the text of the dataset description provided by BCO-DMO:
Exudate Uptake Incubations - Microbial Abundances (Flow Cytometry)
Dataset Description:
The flow cytometry data in this dataset is the raw data and has not been normalized (as is presented in the manuscript Weber et al., 2022).
Other relevant files and publications:
The targeted and untargeted metabolomics data and metadata associated with this study are located on the MetaboLights database under accession numbers MTBLS2855 and MTBLS3286.
The 16S rRNA gene sequencing data and metadata associated with this study are located on the NCBI Sequence Read Archive (SRA) under BioProject PRJNA739882. BioSample accession numbers are not linked with the data submitted to BCO-DMO because samples for flow cytometry and macronutrients were not always collected at the same time as samples collected for microbial community analyses, meaning that only some of the samples collected for microbial community analyses have affiliated microbial abundances and nutrient concentrations.
The following is the text of the dataset description provided by BCO-DMO:
Exudate Uptake Incubations - Microbial Abundances (Flow Cytometry)
Dataset Description:
The flow cytometry data in this dataset is the raw data and has not been normalized (as is presented in the manuscript Weber et al., 2022).
Other relevant files and publications:
The targeted and untargeted metabolomics data and metadata associated with this study are located on the MetaboLights database under accession numbers MTBLS2855 and MTBLS3286.
The 16S rRNA gene sequencing data and metadata associated with this study are located on the NCBI Sequence Read Archive (SRA) under BioProject PRJNA739882. BioSample accession numbers are not linked with the data submitted to BCO-DMO because samples for flow cytometry and macronutrients were not always collected at the same time as samples collected for microbial community analyses, meaning that only some of the samples collected for microbial community analyses have affiliated microbial abundances and nutrient concentrations.
Dataset Citation
- Cite as: Apprill, Amy; Kujawinski, Elizabeth; Gray, Laura (2024). Incubation experiments were conducted in St. John, US Virgin Islands to investigate the response of reef seawater microbial communities to the mixed exudates released from the coral species Porites astreoides and Gorgonia ventalina on 2022-11-10 (NCEI Accession 0291732). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0291732. Accessed [date].
Dataset Identifiers
ISO 19115-2 Metadata
gov.noaa.nodc:0291732
Download Data |
|
Distribution Formats |
|
Ordering Instructions | Contact NCEI for other distribution options and instructions. |
Distributor |
NOAA National Centers for Environmental Information +1-301-713-3277 NCEI.Info@noaa.gov |
Dataset Point of Contact |
NOAA National Centers for Environmental Information ncei.info@noaa.gov |
Time Period | 2022-11-10 to 2022-11-10 |
Spatial Bounding Box Coordinates |
West: -64.7274134835
East: -64.7274134835
South: 18.3157734768
North: 18.3157734768
|
Spatial Coverage Map |
General Documentation |
|
Associated Resources |
|
Publication Dates |
|
Data Presentation Form | Digital table - digital representation of facts or figures systematically displayed, especially in columns |
Dataset Progress Status | Complete - production of the data has been completed Historical archive - data has been stored in an offline storage facility |
Data Update Frequency | As needed |
Supplemental Information | Acquisition Description: Experiments were conducted to monitor responses of reef seawater microbial communities to concentrated exudates from P. astreoides and G. ventalina . After the organism incubations were conducted, excess filtrate (2 l) from 3 of the 6 colony/fragment incubations (selected randomly) were pooled into an acid-washed, 10 l carboy and mixed. Excess filtrate (~2 l) from the three control incubations were also pooled into a second, acid-washed, 10 l carboy and mixed. For the P. astreoides experiment, surface seawater was collected from the offshore site and coarsely filtered through a GF/A filter (1.6 µm nominal pore size) using peristalsis to remove larger cells and minimize heterotrophic grazing, but retain bacteria and archaea. Approximately 2.4 l of this inoculum was added separately to each of the 10 l carboys (pooled coral and control filtrate) to create a 5:2 ratio of filtrate: inoculum. After this addition, each carboy was mixed and a suite of samples were collected for different analyses including cell counts, inorganic and organic macronutrient quantification, and 16S rRNA gene sequencing. This collection marked the first timepoint (0 hours [hrs]) for the exudate uptake experiment. For the G. ventalina experiment, reef seawater inoculum was collected from Tektite reef (Table S1). For each experiment, coral metabolite or control filtrate seawater were transferred into separate 1 l acid-washed polycarbonate bottles (6 bottles per treatment). Within each treatment (coral and control), 3 of the 6 bottles were blackened to block light. The bottles were placed into a flow-through seawater table. PAR readings in the seawater table for the P. astreoides and G. ventalina experiments ranged from 250 – 1000 and 164 - 530 µmol quanta m -2s -1, respectively, with variation caused by shading and cloud cover. Over the course of 48 hours, samples were collected for cell counts (1 ml) at all timepoints (0, 12, 24, 36, and 48 hrs), macronutrient analyses (30 – 40 ml) at 0 and 48 hrs, and microbial community analyses (60 – 300 ml) at 0, 24, and 48 hrs. Samples collected for cell counts were fixed to 1% (v/v) paraformaldehyde, refrigerated for 20 minutes in the dark, and frozen in a charged dry shipper. Seawater samples (60 ml for P. astreoides ) collected for microbial community analyses were obtained using 60 ml sterile, Luer-lock syringes (Becton Dickinson, Franklin Lakes, NJ, USA) and filtered using positive pressure onto 25 mm, 0.2 m pore-size filters. The amount of volume filtered was increased for the G. ventalina exudate experiment because of biomass concerns and ranged from 180 (0 and 24 hrs) to 300 ml (48 hrs). Previous work has demonstrated that sample volume does not significantly influence microbial community composition when beta diversity comparisons are made [6]. Alpha diversity analysis was avoided due to the differences in seawater volumes. Filters were then transferred into cryovials and placed in the charged dry shipper, followed by -80 °C storage until DNA extraction. |
Purpose | This dataset is available to the public for a wide variety of uses including scientific research and analysis. |
Use Limitations |
|
Dataset Citation |
|
Cited Authors | |
Principal Investigators | |
Contributors | |
Resource Providers | |
Points of Contact | |
Publishers | |
Acknowledgments |
Theme keywords |
NODC DATA TYPES THESAURUS
NODC OBSERVATION TYPES THESAURUS
WMO_CategoryCode
|
Data Center keywords | NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS Global Change Master Directory (GCMD) Data Center Keywords |
Instrument keywords | BCO-DMO Standard Instruments Originator Instrument Names |
Project keywords | BCO-DMO Standard Projects Provider Funding Award Information |
Keywords | NCEI ACCESSION NUMBER |
Use Constraints |
|
Data License | |
Access Constraints |
|
Fees |
|
Lineage information for: dataset | |
---|---|
Processing Steps |
|
Output Datasets |
|
Last Modified: 2024-05-31T15:15:28Z
For questions about the information on this page, please email: ncei.info@noaa.gov
For questions about the information on this page, please email: ncei.info@noaa.gov