An investigation of Symbiodiniaceae communities via ITS-2 rDNA amplicon sequencing in Acropora millepora corals from the Great Barrier Reef following exposure to stressors in October 2014 (NCEI Accession 0291727)

This dataset contains biological and survey - biological data collected in the Coral Sea from 2014-10-01 to 2014-10-08. These data include species. The instruments used to collect these data include Automated DNA Sequencer. These data were collected by Adrienne M.S. Correa of Rice University as part of the "Collaborative Research: Viral Reefscapes: The Role of Viruses in Coral Reef Health, Disease, and Biogeochemical Cycling (Moorea Virus Project)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2022-02-09.

The following is the text of the dataset description provided by BCO-DMO:

Acquisition Description:
Symbiodiniaceae communities from Acropora millepora corals were investigated at three locations on the Great Barrier Reef between October 1st and 8th, 2014. Samples from Davies Reef lagoon, Rib Reef, and Pandora Island were collected and snap frozen in liquid nitrogen and stored at -20℃ until further processing. DNA was extracted using Wayne's Method (Wilson et al., 2002; Lundgren et al., 2013) from tissue slurry prepared following the method of Wright et al. (2019).

This dataset lists accessions and collection information for ITS-2 rDNA amplicon data from Acropora millepora corals following exposure to various stressors. Stressors included pCO2, heat, bacteria, all of these, or none of these (control). ITS-2 rDNA Symbiodiniaceae community libraries were prepared and PE 300bp reads were generated using Illumina MiSeq platform. BioSamples and SRA accessions are available at the National Center for Biotechnology Information (NCBI) under BioProject PRJNA596498 (https://www.ncbi.nlm.nih.gov/search/all/?term=PRJNA596498)