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Dataset Overview | National Centers for Environmental Information (NCEI)

The global proteome of replete laboratory cultures of Ruergeria pomeroyi DSS-3 from 2021-03-01 to 2021-05-01 (NCEI Accession 0291609)

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This dataset contains biological and chemical data collected from 2021-03-01 to 2021-05-01. These data include protein. The instruments used to collect these data include High Performance Liquid Chromatograph and Mass Spectrometer. These data were collected by Mary Ann Moran of University of Georgia and Mak A. Saito of Woods Hole Oceanographic Institution as part of the "C-CoMP Model Bacteria Physiological Studies (C-CoMP Model Bacteria)" project and "Center for Chemical Currencies of a Microbial Planet (C-CoMP)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2022-06-15.

The following is the text of the dataset description provided by BCO-DMO:

Ruergeria pomeroyi DSS-3 replete proteome

Dataset Description:
Acquisition Description:
Cultures (50 ml each) of Ruergeria pomeroyi DSS-3 and Alteromonas macleodii MIT1002 were harvested in triplicate, centrifuged at 8000 x g for 10 minutes, flash frozen, stored at -80 degrees C, and shipped to Woods Hole Oceanographic Institution for proteomics analysis. Cultures were filtered and filters were extracted using SDS detergent and a SP3 magnetic bead purification followed by trypsin digestion alkylation and reduction. The
  • Cite as: Saito, Mak A.; Moran, Mary Ann (2024). The global proteome of replete laboratory cultures of Ruergeria pomeroyi DSS-3 from 2021-03-01 to 2021-05-01 (NCEI Accession 0291609). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0291609. Accessed [date].
gov.noaa.nodc:0291609
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Distributor NOAA National Centers for Environmental Information
+1-301-713-3277
NCEI.Info@noaa.gov
Dataset Point of Contact NOAA National Centers for Environmental Information
ncei.info@noaa.gov
Time Period 2021-03-01 to 2021-05-01
Spatial Bounding Box Coordinates
West:
East:
South:
North:
Spatial Coverage Map
General Documentation
Associated Resources
  • Biological, chemical, physical, biogeochemical, ecological, environmental and other data collected from around the world during historical and contemporary periods of biological and chemical oceanographic exploration and research managed and submitted by the Biological and Chemical Oceanography Data Management Office (BCO-DMO)
    • NCEI Collection
      Navigate directly to the URL for data access and direct download.
  • Saito, M. A., Moran, M. A. (2022) The global proteome of replete laboratory cultures of Ruergeria pomeroyi DSS-3. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-06-14. https://doi.org/10.26008/1912/bco-dmo.875600.1
    • Parent ID (indicates this dataset is related to other data):
    • gov.noaa.nodc:BCO-DMO
Publication Dates
  • publication: 2024-04-21
Data Presentation Form Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status Complete - production of the data has been completed
Historical archive - data has been stored in an offline storage facility
Data Update Frequency As needed
Supplemental Information
Methods References:
Saito et al. 2020 and were modified from the methods originally published by Hughes et al. 2014. Analysis was conducted by 2D active modulation Orbitrap mass spectrometry as described in McIlvin and Saito 2021.

This dataset reports results for the Ruergeria pomeroyi DSS-3 samples. See related datasets for the Alteromonas macleodii MIT1002 samples.

Note: the sample ids ending in a1, b1, and c1 differentiate biological triplicates from one another.
Purpose This dataset is available to the public for a wide variety of uses including scientific research and analysis.
Use Limitations
  • accessLevel: Public
  • Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.
Dataset Citation
  • Cite as: Saito, Mak A.; Moran, Mary Ann (2024). The global proteome of replete laboratory cultures of Ruergeria pomeroyi DSS-3 from 2021-03-01 to 2021-05-01 (NCEI Accession 0291609). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0291609. Accessed [date].
Cited Authors
Principal Investigators
Contributors
Resource Providers
Points of Contact
Publishers
Acknowledgments
Theme keywords NODC DATA TYPES THESAURUS NODC OBSERVATION TYPES THESAURUS WMO_CategoryCode
  • oceanography
BCO-DMO Standard Parameters Global Change Master Directory (GCMD) Science Keywords Originator Parameter Names
Data Center keywords NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS Global Change Master Directory (GCMD) Data Center Keywords
Instrument keywords NODC INSTRUMENT TYPES THESAURUS BCO-DMO Standard Instruments Global Change Master Directory (GCMD) Instrument Keywords Originator Instrument Names
Project keywords BCO-DMO Standard Programs BCO-DMO Standard Projects Provider Funding Award Information
Keywords NCEI ACCESSION NUMBER
Use Constraints
  • Cite as: Saito, Mak A.; Moran, Mary Ann (2024). The global proteome of replete laboratory cultures of Ruergeria pomeroyi DSS-3 from 2021-03-01 to 2021-05-01 (NCEI Accession 0291609). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0291609. Accessed [date].
Data License
Access Constraints
  • Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
Fees
  • In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.
Lineage information for: dataset
Processing Steps
  • 2024-04-21T21:44:44Z - NCEI Accession 0291609 v1.1 was published.
Output Datasets
Acquisition Information (collection)
Instrument
  • chromatograph
  • mass spectrometer
Last Modified: 2024-05-31T18:50:46Z
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