Organic polymer formation and domoic acid adsorption from experiments conducted using water samples collected in northern Gulf of Mexico in 2018 and 2019 (NCEI Accession 0291601)
This dataset contains biological and chemical data collected in the Gulf of Mexico from 2018-11-13 to 2019-02-07. These data include domoic acid and particulate organic Carbon (POC). The instruments used to collect these data include Costech International Elemental Combustion System (ECS) 4010, Homogenizer, and Mass Spectrometer. These data were collected by Jeffrey W Krause of Dauphin Island Sea Lab and Kanchan Maiti of Louisiana State University as part of the "The biotic and abiotic controls on the Silicon cycle in the northern Gulf of Mexico (CLASiC)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2020-07-14.
The following is the text of the dataset description provided by BCO-DMO:
Dataset Description: Organic polymer formation and domoic acid adsorption. Results from lab experiments designed to investigate organic polymer formation and domoic acid adsorption. Water samples were collected in the northern Gulf of Mexico in 2018 and 2019.
Related datasets: Domoic acid assimilation in copepods https://www.bco-dmo.org/dataset/808402 Field domoic acid and copepods https://www.bco-dmo.org/dataset/808413
The following is the text of the dataset description provided by BCO-DMO:
Dataset Description: Organic polymer formation and domoic acid adsorption. Results from lab experiments designed to investigate organic polymer formation and domoic acid adsorption. Water samples were collected in the northern Gulf of Mexico in 2018 and 2019.
Related datasets: Domoic acid assimilation in copepods https://www.bco-dmo.org/dataset/808402 Field domoic acid and copepods https://www.bco-dmo.org/dataset/808413
Dataset Citation
- Cite as: Krause, Jeffrey W.; Maiti, Kanchan (2024). Organic polymer formation and domoic acid adsorption from experiments conducted using water samples collected in northern Gulf of Mexico in 2018 and 2019 (NCEI Accession 0291601). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0291601. Accessed [date].
Dataset Identifiers
ISO 19115-2 Metadata
gov.noaa.nodc:0291601
Download Data |
|
Distribution Formats |
|
Ordering Instructions | Contact NCEI for other distribution options and instructions. |
Distributor |
NOAA National Centers for Environmental Information +1-301-713-3277 NCEI.Info@noaa.gov |
Dataset Point of Contact |
NOAA National Centers for Environmental Information ncei.info@noaa.gov |
Time Period | 2018-11-13 to 2019-02-07 |
Spatial Bounding Box Coordinates |
West: -88.075
East: -87.554
South: 30.25
North: 30.278
|
Spatial Coverage Map |
General Documentation |
|
Associated Resources |
|
Publication Dates |
|
Data Presentation Form | Digital table - digital representation of facts or figures systematically displayed, especially in columns |
Dataset Progress Status | Complete - production of the data has been completed Historical archive - data has been stored in an offline storage facility |
Data Update Frequency | As needed |
Supplemental Information | Acquisition Description: Location Water collection sites in the northern Gulf of Mexico, particularly at the mouth of Mobile Bay and Little Lagoon, AL. Water Collection Briefly, water was collected from the field using a 5-gallon bucket, pre-screened with a 200 µm nitex mesh, and gently poured into 10-20 L carboys and kept in the dark until returning to the laboratory for same-day processing. Terminology dDA – dissolved Domoic Acid pDAa – particulate Domoic Acid (algal fraction) pDAOP – particulate Domoic Acid (bound to organic polymers) cDA – Domoic Acid in copepods POC – Particulate Organic Carbon Organic polymer formation and sorption of DA Seawater organic polymers were formed in controlled laboratory conditions to verify whether they could scavenge dDA. Surface seawater was collected from Dauphin Island (AL, USA) and filtered through a new 0.2 µm polycap filter (Pall Brand, USA). The freshly filtered seawater was partitioned into 1-L polycarbonate bottles and the initial measurements of dDA, pDA, cDA, pDAOP, and POC were made. POC was used as a proxy for organic polymer formation. The same filtration techniques were used for dDA and pDA as described above. Laboratory-reared adult Acartia tonsa were collected on a 200 µm screen and gently rinsed with freshly filtered artificial seawater, then transferred into 2 mL cryovials and stored in -20°C until analyzed for cDA. Twenty-five mm glass fiber filters were pre-combusted at 500°C for four hours and used to collect organic polymers. The organic polymer collection method was modified from Passow et al. (1995); loss of organic polymers via filtration was minimized by maintaining low-vacuum (<200 mbar) and filtering samples for a maximum of 15 minutes. Lastly, the treatment bottles were spiked with a DA standard to bring the final concentration to 10 µg DA L-1 and, for specific treatment bottles, 30 copepods were added to each bottle (for copepod data see dataset https://www.bco-dmo.org/dataset/808402). Bottles were then placed on orbital shaker tables and gently shaken for 24 hours. Controls were not shaken. Samples for dDA, pDA, cDA, pDAOP, and POC were collected after the 24-hour time period. Liquid chromatography-mass spectrometry method for domoic acid quantification LC-MS sample preparation followed was modified from Wang et al. (2012) for the determination of dDA, pDA, pDAOP and cDA. The samples for DA determination were cleaned and concentrated using Bond Elut LRC - C18, 200 mg, solid-phase extraction (SPE) columns from Agilent Technologies. For dDA, 30 mL seawater samples were filtered using a 47 mm glass fiber filter; the filtrate was collected and acidified with formic acid to yield a 0.2% final solution. SPE columns were conditioned with one column volume of HPLC-grade methanol followed by one column volume of HPLC-grade water. Samples were then loaded on the SPE column and filtered at ~1 mL min-1 using a vacuum manifold, followed by 10 mL of 0.2% formic acid as a rinse for the sample tube and SPE column. The SPE column was then allowed to go dry and was eluted with 1.5 mL of 20 mM ammonium acetate in 50% methanol (pH 8) and collected in a glass tube. The tubes were centrifuged for 5 minutes at ~1300 x g, supernatant was transferred into an LC vial with a Pasteur pipette, and stored at 4°C until further analysis. For pDAa 100 mL of seawater were filtered through a 5 µm polycarbonate filter and stored in a 50 mL polypropylene tube at -20°C. Similarly, for pDAOP 150 mL of seawater was filtered through a pre-combusted 25 mm glass-fiber filter and stored at -20°C. Prior to concentration and clean-up for pDA, pDAOP, and cDA, the filters were submerged in 2 mL of 80% methanol and sonicated to ensure cells and copepods were lysed. Sonication pulses were done for a total of 45 seconds (5 seconds on/off) on a Sonics Materials Ultrasonic Processor (model - VCX 130) at 75% power. Subsequent clean-up using the SPE column is the same as for the dDA samples. An ultra-performance liquid chromatography (UPLC) – tandem mass spectrometry (MS) system was used for the quantification of DA.The LC-MS system consisted of Acquity UPLC system (Waters, Milford, MA) coupled to a 5500 QTRAP triple quadrupole / linear ion trap mass spectrometer equipped with a TurboIonSpray interface (Sciex, Foster City, CA, USA). The analytes were separated on a Luna C18 (2), 2.0 x 100 mm column (Phenomenex, Torrance, CA, USA) with column temperature held at 40ºC. The mobile phase was water (A) and 95% aqueous acetonitrile (B) with 0.1% formic acid additive and the flow rate was 0.4 ml/min. Gradient program was: 5% B for 3 min, linear gradient to 60% B at 10 min, 95% B at 10.1 min, hold at 95% B for 2 min. MS was operated in positive ion mode. Ion spray voltage was 5 kV and declustering potential was 80 V. Gas parameter settings were: nebulizer gas, 50 psi; turbo gas, 50 psi at 500ºC; curtain gas, 20 psi; and collision gas, medium setting. The collision energy applied was 25eV. The transitions used for selected reaction monitoring were m/z 312→266, 193, 220. The transition m/z 312→266 was used for quantitation. For field-simulation experiment results and methodology see dataset https://www.bco-dmo.org/dataset/808413 |
Purpose | This dataset is available to the public for a wide variety of uses including scientific research and analysis. |
Use Limitations |
|
Dataset Citation |
|
Cited Authors | |
Principal Investigators | |
Contributors | |
Resource Providers | |
Points of Contact | |
Publishers | |
Acknowledgments |
|
Theme keywords |
NODC DATA TYPES THESAURUS
NODC OBSERVATION TYPES THESAURUS
WMO_CategoryCode
|
Data Center keywords | NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS Global Change Master Directory (GCMD) Data Center Keywords |
Instrument keywords | NODC INSTRUMENT TYPES THESAURUS BCO-DMO Standard Instruments Global Change Master Directory (GCMD) Instrument Keywords Originator Instrument Names |
Place keywords | NODC SEA AREA NAMES THESAURUS Global Change Master Directory (GCMD) Location Keywords |
Project keywords | BCO-DMO Standard Projects Provider Funding Award Information |
Keywords | NCEI ACCESSION NUMBER |
Use Constraints |
|
Data License | |
Access Constraints |
|
Fees |
|
Lineage information for: dataset | |
---|---|
Processing Steps |
|
Output Datasets |
|
Acquisition Information (collection) | |
---|---|
Instrument |
|
Last Modified: 2024-06-04T18:34:55Z
For questions about the information on this page, please email: ncei.info@noaa.gov
For questions about the information on this page, please email: ncei.info@noaa.gov