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Oxygen consumption rates/zero valent iron dissolution of FeOB with kanamycin addition - replacement samples from 2016-11-02 to 2016-11-06 (NCEI Accession 0291593)

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This dataset contains chemical data collectedat Intertidal coastal river and coastal shelf sediments, mid-coast, Maine, USA; Monteray Bay Canyon, sediments, CA, USA from 2016-11-02 to 2016-11-06. These data include dissolved Oxygen. The instruments used to collect these data include Optode. These data were collected by Dr David Emerson of Bigelow Laboratory for Ocean Sciences and Dr Peter Girguis of Harvard University as part of the "Collaborative Research: The Role of Iron-oxidizing Bacteria in the Sedimentary Iron Cycle: Ecological, Physiological and Biogeochemical Implications (SedimentaryIronCycle)" project and "Center for Dark Energy Biosphere Investigations (C-DEBI)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-03-26.

The following is the text of the dataset description provided by BCO-DMO:

Oxygen consumption rates/zero valent iron dissolution of FeOB with kanamycin addition - replacement samples

Dataset Description:
In these experiments, oxygen consumption rates were measured for the iron-oxidizing bacteria (FeOB) Mariprofundus ferrooxydans PV-1 as well as the neutrophilic FeOB "TAG-1" (An isolate from hydrothermal vents in the Mid Atlantic ridge that has not yet been formally named. TAG simply refers to the TAG hydrothermal site from which this microbe was isolated.). These experiments measure oxygen consumption at 100 uM oxygen at 20 degrees C. After 3 days the media in each vessel was replaced with fresh media containing kanamycin to stop any further growth and examine abiotic effects of iron mat production on zero valent iron dissolution.
  • Cite as: Girguis, Peter; Emerson, David (2024). Oxygen consumption rates/zero valent iron dissolution of FeOB with kanamycin addition - replacement samples from 2016-11-02 to 2016-11-06 (NCEI Accession 0291593). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0291593. Accessed [date].
gov.noaa.nodc:0291593
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Distributor NOAA National Centers for Environmental Information
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Dataset Point of Contact NOAA National Centers for Environmental Information
ncei.info@noaa.gov
Coverage Description Intertidal coastal river and coastal shelf sediments, mid-coast, Maine, USA; Monteray Bay Canyon, sediments, CA, USA
Time Period 2016-11-02 to 2016-11-06
Spatial Bounding Box Coordinates
West:
East:
South:
North:
Spatial Coverage Map
General Documentation
Associated Resources
  • Biological, chemical, physical, biogeochemical, ecological, environmental and other data collected from around the world during historical and contemporary periods of biological and chemical oceanographic exploration and research managed and submitted by the Biological and Chemical Oceanography Data Management Office (BCO-DMO)
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  • Girguis, P., Emerson, D. (2017) Oxygen consumption rates/zero valent iron dissolution of FeOB with kanamycin addition - replacement samples. Biological and Chemical Oceanography Data Management Office (BCO-DMO). Dataset version 2017-07-21. https://doi.org/10.1575/1912/bco-dmo.709573.1
    • Parent ID (indicates this dataset is related to other data):
    • gov.noaa.nodc:BCO-DMO
Publication Dates
  • publication: 2024-04-21
Data Presentation Form Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status Complete - production of the data has been completed
Historical archive - data has been stored in an offline storage facility
Data Update Frequency As needed
Supplemental Information
Acquisition Description:
These data were collected by placing each strain in a 100 mL serum vial with 6 mL of their standard, published media with 30 mg zero valent iron as a source of Fe(II). The headspace was filled with a gas mix of 8% oxygen/10% carbon dioxide/82% nitrogen by using bottled gas mixes and a regulator to flush the headspace without over pressurization. Prior to sealing the serum vials, a Presens OPTODE dot (sensor) was placed inside the vial, allowing non-invasive gas sampling of the changes in O2 in the headspace. A Presens four channel system was used to measure changes in oxygen concentration in real-time in each bottle. A total of four channels were measured during each experiment: channels 1 through 3 are the biological treatments and channel 4 was a kill control (microbes were by placing on a heat block at 100 degrees C for 5 minutes). After 3 days of incubation, the concentration of Fe(II) in the media was measured by ferrozine assay. Old media was then removed and replaced with fresh media containing 30 ng/ml kanamycin to prevent growth of remaining cells. The headspace was again filled with the same gas mix and oxygen concentrations were measured in real-time in each vial. Fe(II) concentration was determined daily by ferrozine assay for 3 more days.
Purpose This dataset is available to the public for a wide variety of uses including scientific research and analysis.
Use Limitations
  • accessLevel: Public
  • Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.
Dataset Citation
  • Cite as: Girguis, Peter; Emerson, David (2024). Oxygen consumption rates/zero valent iron dissolution of FeOB with kanamycin addition - replacement samples from 2016-11-02 to 2016-11-06 (NCEI Accession 0291593). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0291593. Accessed [date].
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Theme keywords NODC DATA TYPES THESAURUS NODC OBSERVATION TYPES THESAURUS WMO_CategoryCode
  • oceanography
BCO-DMO Standard Parameters Global Change Master Directory (GCMD) Science Keywords Originator Parameter Names
Data Center keywords NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS Global Change Master Directory (GCMD) Data Center Keywords
Instrument keywords BCO-DMO Standard Instruments Originator Instrument Names
Project keywords BCO-DMO Standard Programs BCO-DMO Standard Projects Provider Funding Award Information
Keywords NCEI ACCESSION NUMBER
Use Constraints
  • Cite as: Girguis, Peter; Emerson, David (2024). Oxygen consumption rates/zero valent iron dissolution of FeOB with kanamycin addition - replacement samples from 2016-11-02 to 2016-11-06 (NCEI Accession 0291593). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0291593. Accessed [date].
Data License
Access Constraints
  • Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
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  • In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.
Lineage information for: dataset
Processing Steps
  • 2024-04-21T18:40:57Z - NCEI Accession 0291593 v1.1 was published.
Output Datasets
Last Modified: 2024-05-31T18:50:46Z
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