Acartia tonsa survival data for transgenerational ocean warming and acidification data on 2023-09-07 (NCEI Accession 0291592)

This dataset contains chemical data collectedat North western Atlantic ocean; Gulf of Maine, coastal and estuarine habitats on 2023-09-07. These data include pH. The instruments used to collect these data include Microscope - Optical. These data were collected by Hannes Baumann, Hans G. Dam, and Michael Finiguerra of University of Connecticut and Melissa Pespeni and Reid Brennan of University of Vermont as part of the "Collaborative Research: Response of marine copepods to warming temperature and ocean acidification (Copepod Response to Warming Temp and OA)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2023-09-07.

The following is the text of the dataset description provided by BCO-DMO:

Ocean warming & acidification experiment: Acartia tonsa survival

Dataset Description:
These data are part of at multigenerational experiment of Arcatia tonsa exposure to ocean warming (OW), ocean acidification (OA), and combined ocean warming and acidification (OWA) including a benign ambient condition temperature and CO2 control (AM). These data were collected every third generation between F0 and F15 and at F25 for all treatments.
Methods and Sampling:
Survival was measured from nauplius 1 (N1) stage to copepodid 6 (adult) stage. For a given generation, all adults from the previous generation were removed from the culture and allowed to lay eggs in food-replete media for 48 h. Resulting nauplii were chosen for tracking survival. Unhatched eggs and any nauplii not chosen for survival analysis were returned to their respective replicate cultures for continued population maintenance. To measure survival for all generations where life-history traits were evaluated, three 250-mL beakers for each replicate culture were supplied with 25 randomly chosen N1 nauplii each and housed in the plexiglass enclosure described above (n = 21 per treatment). Copepods were checked every 48-72 h. The number of dead, live, and missing copepods were logged for each beaker along with general stage (i.e. nauplius, copepodite, adult female, or adult male). Nauplii were grown with media at levels of 500 μg C L -1 to prevent overgrowth of phytoplankton and allow for adequate nauplii grazing. Following the naupliar stages, copepods were grown with food-replete (800 μg C L -1 ) media as described earlier. Food was replaced with fresh media on monitoring days. The target (actual ± standard deviation) conditions were as follows: ambient (AM) temperature = 18 °C (18 ± 0.34, N = 330), AM pCO2= 400 μatm (379 ± 36, N = 18; pH = 8.26 ± 0.1, N = 330); high temperature = 22 °C (22 ± 0.81, N = 336); and high pCO2= 2,000 µatm (2,301 ± 215, N = 18; pH = 7.55 ± 0.08, N = 330). AM target levels represented extant conditions for this species in northeast Atlantic estuaries.