Estimates of bleaching mortality and colony size for different Pocillopora haplotypes at Moorea in 2019 (NCEI Accession 0291558)

This dataset contains biological and survey - biological data collected from 2019-02-01 to 2019-02-28. These data include species. These data were collected by Peter J. Edmunds of California State University Northridge and Scott Burgess of Florida State University as part of the "Collaborative research: Coral community resilience: testing the role of hidden diversity in pocilloporid corals at Moorea (Pocilloporid Coral Diversity)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2021-07-26.

The following is the text of the dataset description provided by BCO-DMO:

Haplotypes Moorea 2019

Dataset Description:
Acquisition Description:
Tissue was collected in February 2019 from a total of 68 colonies from Sites 1 (n = 7), 2 (n = 30), 3 (n = 19), and 5 (n = 12). Of these 68 colonies, 51 colonies were tracked between February 2019 (before the bleaching) and August 2019 (after the bleaching). Tissue was collected in August from a total of 394 colonies from Sites 1 (n = 65), 2 (n = 69), 3 (n = 42), 4 (n = 83), 5 (n = 68), and 6 (n = 67).

The mitochondrial Open Reading Frame (mtORF) marker, using the FATP6.1 and RORF primers found in Flot et al. (2008), was used to haplotype individuals. P. meandrina and P. eydouxi were differentiated using a restriction fragment length polymorphism (RFLP) gel-based assay following Johnston et al. (2018). Samples were identified to haplotype based on previously published sequences of mtORF haplotypes, using the naming conventions in Pinzón and LaJeunesse (2011), Pinzón et al. (2013), Forsman et al. (2013), Edmunds et al. (2016), and Johnston et al. (2017).