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Dataset Overview | National Centers for Environmental Information (NCEI)

FlowCAM enumeration of phytoplankton classes from samples taken during R/V Endeavor cruise EN616 in July 2018 (NCEI Accession 0291542)

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This dataset contains biological, chemical, and physical data collected on R/V Endeavor during cruise EN616 in the New York Bight and North Atlantic Ocean from 2018-07-05 to 2018-07-13. These data include biomass carbon and depth. The instruments used to collect these data include Yokogawa Fluid Imaging Technologies FlowCam particle imaging system. These data were collected by David T. Drapeau, Jelena Godrijan, Stephen D. Archer, and William M. Balch of Bigelow Laboratory for Ocean Sciences as part of the "Coccolithophore Mixotrophy (Cocco-Mix)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2023-03-09.

The following is the text of the dataset description provided by BCO-DMO:

Dataset Description:
This dataset is part of a larger study with the following goals:

Goal #1 : measure the mixotrophic uptake and assimilation of 14C-acetate, 14C-mannitol and 14C-glycerol as a carbon source by natural assemblages of coccolithophores and compare it to their autotrophic uptake and assimilation of DIC. These three organics were chosen due to their high potential for significant osmotrophy by coccolithophores as seen in previous culture studies (Godrijan et al. (2020) and BCO-DMO dataset: https://www.bco-dmo.org/dataset/858771 ). The design of these experiments used radiochemical and single cell/flow cytometer methods to distinguish osmotrophy of coccolithophores from that by other naturally-occurring microalgae. Goal #2 : test for the fixation of 14C-labeled organics into both POC and PIC fractions in natural populations of coccolithophores, in order to examine the potential role of coccolithophore osmotrophy in the biological carbon pump and alkalinity carbon pump paradigms.
  • Cite as: Balch, William M.; Archer, Stephen D.; Drapeau, David T.; Godrijan, Jelena (2024). FlowCAM enumeration of phytoplankton classes from samples taken during R/V Endeavor cruise EN616 in July 2018 (NCEI Accession 0291542). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0291542. Accessed [date].
gov.noaa.nodc:0291542
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Distributor NOAA National Centers for Environmental Information
+1-301-713-3277
NCEI.Info@noaa.gov
Dataset Point of Contact NOAA National Centers for Environmental Information
ncei.info@noaa.gov
Time Period 2018-07-05 to 2018-07-13
Spatial Bounding Box Coordinates
West: -72.927
East: -66.517
South: 36.986
North: 43.718
Spatial Coverage Map
General Documentation
Associated Resources
  • Biological, chemical, physical, biogeochemical, ecological, environmental and other data collected from around the world during historical and contemporary periods of biological and chemical oceanographic exploration and research managed and submitted by the Biological and Chemical Oceanography Data Management Office (BCO-DMO)
    • NCEI Collection
      Navigate directly to the URL for data access and direct download.
  • Balch, W. M., Godrijan, J., Drapeau, D. T., Archer, S. D. (2023) FlowCAM enumeration of phytoplankton classes from samples taken during R/V Endeavor cruise EN616 in July 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-01-27. https://doi.org/10.26008/1912/bco-dmo.887787.1
    • Parent ID (indicates this dataset is related to other data):
    • gov.noaa.nodc:BCO-DMO
Publication Dates
  • publication: 2024-04-21
Data Presentation Form Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status Complete - production of the data has been completed
Historical archive - data has been stored in an offline storage facility
Data Update Frequency As needed
Supplemental Information
Acquisition Description:
At sea collections
Nine stations were visited during R/V Endeavor EN616 cruise in the northwest Atlantic.
At eight depths, three 10L Niskin samples were taken for discrete measurements of:

1. Chlorophyll, nutrients including nitrate, nitrite, ammonium, phosphate, and silicate
2. Particulate organic carbon (POC) plus particulate organic nitrogen (PON)
3. Particulate inorganic carbon (PIC)
4. Biogenic silica
5. Birefringence counts of coccolithophores (done ashore)
6. Shipboard Yokogawa Fluid Imaging Technologies FlowCam imaging cytometer, in order to enumerate the major microalgal classes and estimate the particle size distribution function

Measurements 1 to 4 are part of BCO-DMO dataset 837074 (See https://www.bco-dmo.org/dataset/837074 , and the Related Datasets section below).
Measurement 5 of birefringence counts data is BCO-DMO dataset 887863 (See https://www.bco-dmo.org/dataset/887863 , and the Related Datasets section below).
Measurement 6 is flow cytometer data presented in this dataset.

FlowCAM enumeration of various phytoplankton classes
A Yokogowa FlowCAM imaging cytometer was used to enumerate different classes of phytoplankton. The instrument was keyed on particle backscattering and fluorescence properties. Samples were first filtered through 100um Nitex mesh to make sure the 100um diameter flow chamber did not clog. The instrument was run with a 10X objective in order to reliably count particles bigger than 4-5um diameter. Samples were processed according to Poulton and Martin (2010).

Concentrations (per mL), percent contribution with respect to total particles, and biomass are presented. Carbon biomass was determined based on the Menden-Deuer & Lessard (2000) method.
Purpose This dataset is available to the public for a wide variety of uses including scientific research and analysis.
Use Limitations
  • accessLevel: Public
  • Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.
Theme keywords NODC DATA TYPES THESAURUS NODC OBSERVATION TYPES THESAURUS WMO_CategoryCode
  • oceanography
BCO-DMO Standard Parameters Global Change Master Directory (GCMD) Science Keywords Originator Parameter Names
Data Center keywords NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS Global Change Master Directory (GCMD) Data Center Keywords
Platform keywords NODC PLATFORM NAMES THESAURUS BCO-DMO Platform Names Global Change Master Directory (GCMD) Platform Keywords ICES/SeaDataNet Ship Codes
Instrument keywords BCO-DMO Standard Instruments Originator Instrument Names
Place keywords NODC SEA AREA NAMES THESAURUS Global Change Master Directory (GCMD) Location Keywords Provider Place Names
Project keywords BCO-DMO Standard Projects Provider Cruise IDs Provider Funding Award Information
Keywords NCEI ACCESSION NUMBER
Use Constraints
  • Cite as: Balch, William M.; Archer, Stephen D.; Drapeau, David T.; Godrijan, Jelena (2024). FlowCAM enumeration of phytoplankton classes from samples taken during R/V Endeavor cruise EN616 in July 2018 (NCEI Accession 0291542). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0291542. Accessed [date].
Data License
Access Constraints
  • Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
Fees
  • In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.
Lineage information for: dataset
Processing Steps
  • 2024-04-21T14:57:11Z - NCEI Accession 0291542 v1.1 was published.
Output Datasets
Acquisition Information (collection)
Platform
  • ENDEAVOR
Last Modified: 2024-05-31T18:50:46Z
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