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Dataset Overview | National Centers for Environmental Information (NCEI)

Fluorescence spectra for 3 strains of Synechococcus while increasing temperatures to detect the photosystem components disassociation temperature from 2017-01-01 to 2018-10-31 (NCEI Accession 0291524)

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This dataset contains biological, optical, and physical data collected from 2017-01-01 to 2018-10-31. These data include fluorescence, water temperature, and wavelength. The instruments used to collect these data include Centrifuge and plate reader. These data were collected by David A. Hutchins of University of Southern California as part of the "Dimensions: Collaborative Research: Genetic, functional and phylogenetic diversity determines marine phytoplankton community responses to changing temperature and nutrients (Phytoplankton Community Responses)" project and "Dimensions of Biodiversity (Dimensions of Biodiversity)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2020-03-09.

The following is the text of the dataset description provided by BCO-DMO:

Synechococcus fluorescence emission spectra

Dataset Description:
Fluorescence spectra from 600-700nm at 530nm excitation for 3 strains of Synechococcus while increasing temperatures to detect the photosystem components disassociation temperature
  • Cite as: Hutchins, David A. (2024). Fluorescence spectra for 3 strains of Synechococcus while increasing temperatures to detect the photosystem components disassociation temperature from 2017-01-01 to 2018-10-31 (NCEI Accession 0291524). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0291524. Accessed [date].
gov.noaa.nodc:0291524
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Distributor NOAA National Centers for Environmental Information
+1-301-713-3277
NCEI.Info@noaa.gov
Dataset Point of Contact NOAA National Centers for Environmental Information
ncei.info@noaa.gov
Time Period 2017-01-01 to 2018-10-31
Spatial Bounding Box Coordinates
West: -71.4007
East: -71.2674
South: 41.4471
North: 41.7129
Spatial Coverage Map
General Documentation
Associated Resources
  • Biological, chemical, physical, biogeochemical, ecological, environmental and other data collected from around the world during historical and contemporary periods of biological and chemical oceanographic exploration and research managed and submitted by the Biological and Chemical Oceanography Data Management Office (BCO-DMO)
    • NCEI Collection
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  • Hutchins, D. A. (2019) Fluorescence spectra for 3 strains of Synechococcus while increasing temperatures to detect the photosystem components disassociation temperature. Biological and Chemical Oceanography Data Management Office (BCO-DMO). Dataset version 2019-11-20. https://doi.org/10.1575/1912/bco-dmo.782322.1
    • Parent ID (indicates this dataset is related to other data):
    • gov.noaa.nodc:BCO-DMO
Publication Dates
  • publication: 2024-04-21
Data Presentation Form Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status Complete - production of the data has been completed
Historical archive - data has been stored in an offline storage facility
Data Update Frequency As needed
Supplemental Information
Acquisition Description:
Natural seawater was enriched for photoautotrophs and split into multiple temperatures for two weeks. After the enrichment period, Synechococcus was isolated from each temperature. Each isolate's thermal niche was measured through a series of lab experiments and sequenced.

200ml of dense culture was concentrated by centrifuging 25,000 x g for 15 minutes. The subsequent cell pellet was resuspended in 5ml of sterile growth media. Cell concentrate was dark acclimated for 10 minutes and 200ul was used to measure the fluorescence spectra. After each measurement, the temperature increased and cells were dark acclimated at that temperature for an additional 10 minutes. This continued until the fluorescence decreased to ~0 indicating the disassociation of the photosynthetic apparatus. This was done following the methods published in Pittera et al., 2017.
Purpose This dataset is available to the public for a wide variety of uses including scientific research and analysis.
Use Limitations
  • accessLevel: Public
  • Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.
Dataset Citation
  • Cite as: Hutchins, David A. (2024). Fluorescence spectra for 3 strains of Synechococcus while increasing temperatures to detect the photosystem components disassociation temperature from 2017-01-01 to 2018-10-31 (NCEI Accession 0291524). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0291524. Accessed [date].
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Theme keywords NODC DATA TYPES THESAURUS NODC OBSERVATION TYPES THESAURUS WMO_CategoryCode
  • oceanography
BCO-DMO Standard Parameters Global Change Master Directory (GCMD) Science Keywords Originator Parameter Names
Data Center keywords NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS Global Change Master Directory (GCMD) Data Center Keywords
Instrument keywords BCO-DMO Standard Instruments Originator Instrument Names
Project keywords BCO-DMO Standard Programs BCO-DMO Standard Projects Provider Funding Award Information
Keywords NCEI ACCESSION NUMBER
Use Constraints
  • Cite as: Hutchins, David A. (2024). Fluorescence spectra for 3 strains of Synechococcus while increasing temperatures to detect the photosystem components disassociation temperature from 2017-01-01 to 2018-10-31 (NCEI Accession 0291524). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0291524. Accessed [date].
Data License
Access Constraints
  • Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
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  • In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.
Lineage information for: dataset
Processing Steps
  • 2024-04-21T13:50:02Z - NCEI Accession 0291524 v1.1 was published.
Output Datasets
Last Modified: 2024-05-31T15:15:28Z
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