The thermal niche for each of 11 new isolates of marine Synechococcus from Narragansett Bay, July 2017 (NCEI Accession 0291508)
This dataset contains biological and physical data collected from 2017-01-01 to 2018-10-31. These data include taxon_code and water temperature. The instruments used to collect these data include Turner Designs Fluorometer -10-AU. These data were collected by David A. Hutchins of University of Southern California as part of the "Dimensions: Collaborative Research: Genetic, functional and phylogenetic diversity determines marine phytoplankton community responses to changing temperature and nutrients (Phytoplankton Community Responses)" project and "Dimensions of Biodiversity (Dimensions of Biodiversity)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2020-04-27.
The following is the text of the dataset description provided by BCO-DMO:
Synechococcus thermal niche calcs
Dataset Description:
The thermal niche for each of 11 new isolates of marine Synechococcus from Narragansett Bay, July 2017.
The following is the text of the dataset description provided by BCO-DMO:
Synechococcus thermal niche calcs
Dataset Description:
The thermal niche for each of 11 new isolates of marine Synechococcus from Narragansett Bay, July 2017.
Dataset Citation
- Cite as: Hutchins, David A. (2024). The thermal niche for each of 11 new isolates of marine Synechococcus from Narragansett Bay, July 2017 (NCEI Accession 0291508). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0291508. Accessed [date].
Dataset Identifiers
ISO 19115-2 Metadata
gov.noaa.nodc:0291508
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Ordering Instructions | Contact NCEI for other distribution options and instructions. |
Distributor |
NOAA National Centers for Environmental Information +1-301-713-3277 NCEI.Info@noaa.gov |
Dataset Point of Contact |
NOAA National Centers for Environmental Information ncei.info@noaa.gov |
Time Period | 2017-01-01 to 2018-10-31 |
Spatial Bounding Box Coordinates |
West: -71.4007
East: -71.2674
South: 41.4471
North: 41.7129
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Data Presentation Form | Digital table - digital representation of facts or figures systematically displayed, especially in columns |
Dataset Progress Status | Complete - production of the data has been completed Historical archive - data has been stored in an offline storage facility |
Data Update Frequency | As needed |
Supplemental Information | Acquisition Description: Natural seawater was enriched for photoautotrophs and split into multiple temperatures for two weeks. After the enrichment period, Synechococcus was isolated from each temperature. Each isolate's thermal niche (Thermal Performance Curve) was measured through a series of lab experiments and sequenced. Methods: Thermal niches were calculated by measuring each strain’s thermal performance curve. This was done by acclimating aliquots of each culture for two weeks to a broad range of temperatures between 9° and 33°. Temperatures >33° were added as needed for strains able to grow at these levels. This temperature range was chosen because it exceeds the current summer high and low temperatures in Narragansett Bay, and encompasses the projected warmer temperatures expected in coming decades. Strains were grown at each temperature in triplicate 8 ml borosilicate vials containing 5ml of F/2 medium. Biomass was recorded every two days using in vivo chlorophyll a fluorescence measured on a Turner AU-10 fluorometer (Turner Designs Inc., Sunnyvale, CA, USA), and growth rates and Eppley-Norberg thermal performance curves (Norberg 2004) were calculated in R (Team 2019) using the package growthTools (https://doi.org/10.5281/zenodo.3634918). Rare cultures containing contaminants (verified using fluorescence microscopy) were excluded from the dataset. In two strains for low temperature treatments, LA20 and LA27, after two weeks of acclimation no Synechococcus cells were observed in the culture, so the growth rate was set to zero for these cultures. The results of these thermal performance curves are hereafter referred to as a strain’s phenotype. |
Purpose | This dataset is available to the public for a wide variety of uses including scientific research and analysis. |
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Data Center keywords | NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS Global Change Master Directory (GCMD) Data Center Keywords |
Instrument keywords | NODC INSTRUMENT TYPES THESAURUS BCO-DMO Standard Instruments Global Change Master Directory (GCMD) Instrument Keywords Originator Instrument Names |
Project keywords | BCO-DMO Standard Programs BCO-DMO Standard Projects Provider Funding Award Information |
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Last Modified: 2024-05-31T15:15:28Z
For questions about the information on this page, please email: ncei.info@noaa.gov
For questions about the information on this page, please email: ncei.info@noaa.gov