Series 3A: Multiple stressor experiments on T. pseudonana (CCMP1014) - Chlorophyll, particulate organic carbon and particulate organic nitrogen from 2018-07-01 to 2018-12-31 (NCEI Accession 0291452)
This dataset contains biological, chemical, optical, and physical data collected from 2018-07-01 to 2018-12-31. These data include Partial pressure of CO2, chlorophyll a, irradiance, particulate organic Carbon (POC), particulate organic nitrogen, and water temperature. The instruments used to collect these data include CHN Elemental Analyzer, Cell Cultivator, and Fluorometer. These data were collected by Dr Edward Laws of Louisiana State University and Dr Uta Passow and Nigel D'Souza of University of California-Santa Barbara as part of the "Collaborative Research: Effects of multiple stressors on Marine Phytoplankton (Stressors on Marine Phytoplankton)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2020-06-29.
The following is the text of the dataset description provided by BCO-DMO:
Series 3A: POC, PON, chl-a
Dataset Description:
The experiments were designed to test the combined effects of CO2, temperatures, and light on growth and photophysiology of the diatom T. pseudonana CCMP1014 in a multifactorial design. This dataset contains measurements of extracted chlorophyll, particulate organic carbon (POC), and particulate organic nitrogen (PON) made over the course of the experiments.
The following is the text of the dataset description provided by BCO-DMO:
Series 3A: POC, PON, chl-a
Dataset Description:
The experiments were designed to test the combined effects of CO2, temperatures, and light on growth and photophysiology of the diatom T. pseudonana CCMP1014 in a multifactorial design. This dataset contains measurements of extracted chlorophyll, particulate organic carbon (POC), and particulate organic nitrogen (PON) made over the course of the experiments.
Dataset Citation
- Cite as: Passow, Uta; Laws, Edward; D'Souza, Nigel (2024). Series 3A: Multiple stressor experiments on T. pseudonana (CCMP1014) - Chlorophyll, particulate organic carbon and particulate organic nitrogen from 2018-07-01 to 2018-12-31 (NCEI Accession 0291452). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0291452. Accessed [date].
Dataset Identifiers
ISO 19115-2 Metadata
gov.noaa.nodc:0291452
Download Data |
|
Distribution Formats |
|
Ordering Instructions | Contact NCEI for other distribution options and instructions. |
Distributor |
NOAA National Centers for Environmental Information +1-301-713-3277 NCEI.Info@noaa.gov |
Dataset Point of Contact |
NOAA National Centers for Environmental Information ncei.info@noaa.gov |
Time Period | 2018-07-01 to 2018-12-31 |
Spatial Bounding Box Coordinates |
West:
East:
South:
North:
|
Spatial Coverage Map |
General Documentation |
|
Associated Resources |
|
Publication Dates |
|
Data Presentation Form | Digital table - digital representation of facts or figures systematically displayed, especially in columns |
Dataset Progress Status | Complete - production of the data has been completed Historical archive - data has been stored in an offline storage facility |
Data Update Frequency | As needed |
Supplemental Information | Acquisition Description: Three CO2 concentrations were tested: 410 ppm, 750 ppm, and 1000 ppm respectively. For each CO2 concentration, four temperatures were tested: 15 degrees-C, 20 degrees-C, 25 degrees-C, and 30 degrees-C. Within each temperature, three light levels were tested: a sub-optimum light (SOL) intensity of 60 umol photons · m-2 · s-1, an optimum light (OL) intensity of 400 umol photons · m-2 · s-1 and an extreme light (EL) intensity of 800 umol photons · m-2 · s-1. All lights were set at a 12 h day: 12 h dark cycle. For logistical reasons, experiments were partially conducted in series, with all light treatments at two temperatures (either 15 degrees-C and 25 degrees-C or 20 degrees-C and 30 degrees-C) running simultaneously. This was repeated for each CO2 concentration. Experiments were conducted in Multicultivator MC-1000 OD units (Photon Systems Instruments, Drasov, Czech Republic). Each unit consists of eight 85 ml test-tubes immersed in a thermostated water bath, each independently illuminated by an array of cool white LEDs set at specific intensity and timing. A 0.2um filtered CO2-air mix (Praxair Distribution Inc.) was bubbled through sterile artificial seawater, and the humidified gas mix was supplied to each tube via gentle sparging through a 2um stainless steel diffuser. Flow rates were gradually increased over the course of the incubation to compensate for the DIC uptake of actively growing cells, and ranged from <0.04 Liters per minute (LPM) at the start of the incubations to 0.08 LPM in each tube after 2 days. For each CO2 and temperature level, replication was achieved by incubating three tubes at sub-optimum light intensities, two tubes at optimum light intensity, and three tubes at extreme light intensities. Each experiment was split into two phases: An acclimation phase spanning 4 days, was used to acclimate cultures to their new environment. Pre-acclimated, exponentially-growing cultures were then inoculated into fresh media and incubated through a 3-day experimental phase during which assessments of growth, photophysiology, and nutrient cycling were carried out daily. All sampling started 5 hours into the daily light cycle to minimize the effects of diurnal cycles. Experiments were conducted with artificial seawater (ASW) prepared using previously described methods (Kester et. al 1967), and enriched with nitrate (NO3), phosphate (PO4), silicic acid (Si[OH]4), at levels ensuring that the cultures would remain nutrient-replete over the course of the experiment. Trace metals and vitamins were added as in f/2 (Guillard 1975). The expected DIC concentration and pH of the growth media was determined for the different pCO2 and temperatures using the CO2SYS calculator (Pierrot et al. 2006), with constants from Mehrbach et al. (1973, refit by Dickson & Millero 1987), and inputs of temperature, salinity, total alkalinity (2376.5 umol · kg-1), pCO2, phosphate, and silicic acid. DIC levels in ASW at the start of each phase of the experiments were manipulated by the addition of NaHCO3, and was then maintained by bubbling a CO2-Air mix through the cultures over the course of the experiments. The pH of the growth media was measured spectrophometrically using the m-cresol purple method (Dickson 1993), and adjusted using 0.1N HCl or 0.1M NaOH. The media was distributed into 75 ml aliquots and each aliquot was inoculated with 5 ml of the T. pseudonana CCMP 1014 (TP1014) stock culture at the start of the experiments. Organic Carbon and Nitrogen concentrations: Samples were filtered onto pre-combusted GF/F filters, dried at 60 degrees C, and stored at room temperature until analyses of particulate organic carbon (POC), and particulate organic nitrogen (PON). Between 3 and > 10 mL were filtered, with larger filtration volumes used on the final day of the experiment. Samples were analyzed using an elemental analyzer (CEC 44OHA; Control Equipment). Samples where C or N concentrations were below instrument detection limits were flagged. Chlorophyll: Daily subsamples from each treatment were filtered onto 0.45 µm polycarbonate filters and stored at -20 degrees C. Filters were placed in 90% acetone (v/v) overnight at -20 degrees C, and the extracted chlorophyll was measured fluorometrically on a Turner 700 fluorometer (Strickland 1972). Chlorophyll-a liquid standards in 90% acetone (Turner Designs Inc.), and adjustable solid secondary standards (Turner Designs Inc. P/N 8000-952) were used for calibrations, and to calculate the chlorophyll content of the samples (Column O) |
Purpose | This dataset is available to the public for a wide variety of uses including scientific research and analysis. |
Use Limitations |
|
Dataset Citation |
|
Cited Authors | |
Principal Investigators | |
Contributors | |
Resource Providers | |
Points of Contact | |
Publishers | |
Acknowledgments |
Use Constraints |
|
Data License | |
Access Constraints |
|
Fees |
|
Lineage information for: dataset | |
---|---|
Processing Steps |
|
Output Datasets |
|
Acquisition Information (collection) | |
---|---|
Instrument |
|
Last Modified: 2024-05-31T15:15:28Z
For questions about the information on this page, please email: ncei.info@noaa.gov
For questions about the information on this page, please email: ncei.info@noaa.gov