Genetic sequence accessions, collection information, and methodology for raw sequences from ezRAD libraries of Pocillopora spp. collected in Moorea, French Polynesia in 2019 (NCEI Accession 0291331)

This dataset contains biological data collected in the South Pacific Ocean on 2019-08-01. These data include depth and taxon. The instruments used to collect these data include Automated DNA Sequencer. These data were collected by Scott Burgess of Florida State University as part of the "Collaborative research: Coral community resilience: testing the role of hidden diversity in pocilloporid corals at Moorea (Pocilloporid Coral Diversity)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2022-10-12.

The following is the text of the dataset description provided by BCO-DMO:

Acquisition Description:
Tissue samples from 44 Pocillopora colonies were collected using SCUBA in August 2019 from six sites (LTER 1 – 6) and three depths (5, 10, and 20 m) around Mo’orea, French Polynesia. Tissue samples were stored in salt-saturated DMSO (dimethyl sulfoxide) buffer until DNA was extracted. Genomic DNA was extracted from tissue using the OMEGA (BIO-TEK) E.Z.N.A. Tissue DNA Kit. Extractions were quantified using the Qubit dsDNA HS Assay Kit with the Qubit Fluorometer. ezRAD libraries were generated by digesting samples with the isoschizomer restriction enzymes MboI and Sau3AI (New England BioLab), which cleave at GATC cut sites, and libraries were generated with the KAPA HyperPrep Kit (Roche) using TruSeq DNA indexes (Illumina). Libraries were size selected at 350 – 700 bp and sequenced on the MiSeq platform as paired-end 300 bp runs at Florida State University.