MtORF haplotype of each Pocillopora colony sampled at a given site and depth at Moorea, French Polynesia in August 2019 (NCEI Accession 0291330)

This dataset contains biological and survey - biological data collected from 2019-08-01 to 2019-08-31. These data include depth and species. The instruments used to collect these data include PCR Thermal Cycler. These data were collected by Peter J. Edmunds of California State University Northridge and Scott Burgess of Florida State University as part of the "Collaborative research: Coral community resilience: testing the role of hidden diversity in pocilloporid corals at Moorea (Pocilloporid Coral Diversity)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2021-08-11.

The following is the text of the dataset description provided by BCO-DMO:

Pocillopora haplotypes at Moorea by site and depth

Dataset Description:
Acquisition Description:
In August 2019, Pocillopora colonies were sampled from three depths (5, 10, and 20 m) at each of four sites (sites 1, 2, 4, and 5) on the fore reef of Mo'orea. Site locations and names correspond to that used by the Mo'orea Coral Reef Long-Term Ecological Research (MCR-LTER) program. At each depth at each site, 10-15 50x50 cm quadrats were randomly placed on the reef along the target depth contour until approximately 50 colonies had been sampled. Tissue from all Pocillopora colonies within the quadrat was sampled. Tissue (~ 5 mm diameter) was collected using small bone clippers, stored in salt-saturated dimethyl sulfoxide DMSO buffer. Genomic DNA was extracted from tissues using Chelex 100 (Bio-Rad, USA). DNA was then used for PCR amplification using the mitochondrial Open Reading Frame (mtORF) marker (Flot & Tillier, 2007). A restriction fragment length polymorphism (RFLP) gel-based assay was used to distinguish P. meandrina and P. eydouxi . In GENEIOUS v.9.1.8 (Biomatters), forward mtORF sequences (855bp) were aligned and samples were identified to haplotype based on previously published sequences of mtORF haplotypes (see the attached Supplemental File 'Pocillopora_mtORF.fasta').