Viral consortia in Stony Coral Tissue Loss Disease- affected, disease-exposed, and disease-unexposed coral colonies from a transmission experiment conducted on samples collected from Rupert’s Rock in St. Thomas, U.S. Virgin Islands in 2019 (NCEI Accession 0291320)

This dataset contains biological and survey - biological data collected in the Caribbean Sea from 2019-04-11 to 2019-04-12. These data include depth and species. The instruments used to collect these data include Centrifuge, Manual Biota Sampler, and Self-Contained Underwater Breathing Apparatus. These data were collected by Adrienne M.S. Correa of Rice University and Laura Mydlarz of University of Texas at Arlington as part of the "RAPID: Collaborative Research: Predicting the Spread of Multi-Species Coral Disease Using Species Immune Traits (Multi-Species Coral Disease)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2022-11-04.

The following is the text of the dataset description provided by BCO-DMO:

Viral Consortia in Stony Corals

Dataset Description:
Acquisition Description:
This dataset represents collections of Montastraea cavernosa , Porites astreoides , and Pseudodiploria strigosa colonies, no larger than 25 centimeters (cm) × 25 centimeters (cm), from Rupert’s Rock (18°19′39.6ʺN 64°55′33.5ʺW) in St. Thomas, U.S. Virgin Islands. The corals were collected by divers on SCUBA with hammers and chisels (March 2019) and preserved for analysis in April 2019.

Methodology:
Samples were preprocessed by Novogene Co., Ltd. (Davis, CA, USA) for mRNA enrichment using polyA tail capture; the mRNA libraries underwent 150-bp, paired-end sequencing on an Illumina NovaSeq 6000 instrument using the NEBNext Ultra II RNA library prep kit.

Sampling and analytical procedures:
Samples were flash frozen in liquid nitrogen and stored at -80 degrees C until further processing. Total RNA was extracted using the RNAqueous-4PCR total RNA isolation kit (Invitrogen, Life Technologies AM1914). Tissues were lysed using a refrigerated Qiagen TissueLyser II microcentrifuge at 30 oscillations per second for 30 seconds. The elution stage consisted of two consecutive 30-milliliter (mL) elutions. Contaminating DNA and chromatin were removed from the total RNA using the Ambion DNase I (RNase-free) kit (Invitrogen, Life Technologies AM2222).

A combination of reads from stony coral tissue loss disease affected, stony coral tissue loss disease exposed and control (unexposed) holobiont metatranscriptomes ( Montastraea cavernosa , Porites astreoides and Pseudodiploria strigosa ) were generated from the following samples: Mcav_c3, Mcav_c6, Mcav_c7, Mcav_d2, Mcav_d3, Mcav_d4, Mcav_d6, Mcav_d8, Past_c6, Past_d4, Past_d6 and Pstrig_d5 and given the NCBI accession number: OM030231.

A combination of reads from stony coral tissue loss disease affected, stony coral tissue loss disease exposed and control (unexposed) holobiont metatranscriptomes ( Montastraea caverno sa, Porites astreoides and Pseudodiploria strigosa ) were generated from the following samples: Mcav_c3, Mcav_c6, Mcav_c7, Mcav_d2, Mcav_d3, Mcav_d4, Mcav_d6, Mcav_d8, Past_c6, Past_d4 and Pstrig_d5 and given the NCBI accession number: OM030232.

Sequence data is available in NCBI Genbank under BioProject accession PRJNA788911 and including NCBI SRA run SRR17230316 - SRR17230326.