Size-fractionated chlorophyll a from multivariate mesocosm experiments conducted with a natural phytoplankton community from Narragansett Bay, RI on 2017-03-20 (NCEI Accession 0291313)

This dataset contains biological and physical data collected on 2017-03-20. These data include chlorophyll a, total phaeopigment, and water temperature. The instruments used to collect these data include Turner Designs Fluorometer 10-AU. These data were collected by Gayantonia Franze of Institute of Marine Research (Bergen, Norway), Paul Wilburn of Michigan State University, Elena Litchman of Mississippi State University, Joshua D. Kling of University of California-Berkeley, Colin T. Kremer of University of California-Los Angeles, Stephanie I. Anderson, Susanne Menden-Deuer, and Tatiana A. Rynearson of University of Rhode Island, and David A. Hutchins of University of Southern California as part of the "Dimensions: Collaborative Research: Genetic, functional and phylogenetic diversity determines marine phytoplankton community responses to changing temperature and nutrients (Phytoplankton Community Responses)" project and "Dimensions of Biodiversity (Dimensions of Biodiversity)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2021-04-28.

The following is the text of the dataset description provided by BCO-DMO:

Acquisition Description:
Multivariate mesocosm experiments were conducted with a natural phytoplankton community from Narragansett Bay, RI. Water was incubated in triplicate at -0.5ºC, 2.6ºC, and 6ºC for 10 days. At each temperature, treatments included both nutrient amendments (N, P, Si addition) and controls (no macronutrients added). For complete methodology, see “The Interactive Effects of Temperature and Nutrients on a Spring Phytoplankton Community” (Anderson et al, in prep ).

Community size structure from incubation experiments was assessed using size-fractionated chlorophyll a. At each dilution time point, whole incubation water was filtered in triplicate onto 25 mm Whatman GF/F filters (GE Healthcare), 5 µm polyester filters, or pre-filtered through a 20 µm mesh and then filtered onto 25 mm GF/F filters. This allowed for the calculation of the following size fractions: 0.7-5 µm, 5-20 µm, and >20 µm. Chlorophyll was then extracted in 90% acetone for 24 hours at -20 º C. Fluorescence was read on a 10-AU fluorometer (Turner Designs) and data was analyzed according to the techniques described in Graff and Rynearson (2011).