Hydrolysis rates from gravity filtered samples, plate reader results from RV/Endeavor EN556, 2015 (Patterns of activities project) (NCEI Accession 0291303)
This dataset contains data collected on R/V Endeavor during cruise EN556 from 2015-04-27 to 2015-05-02. These data include depth. The instruments used to collect these data include Fluorometer, Niskin bottle, and plate reader. These data were collected by Carol Arnosti of University of North Carolina at Chapel Hill as part of the "Latitudinal and depth-related contrasts in enzymatic capabilities of pelagic microbial communities: Predictable patterns in the ocean? (Patterns of activities)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2020-05-13.
The following is the text of the dataset description provided by BCO-DMO:
Gravity filtered polysaccharide hydrolysis rates - EN556
Dataset Description:
This dataset includes polysaccharide hydrolysis rates to measure microbial enzyme activities and bacterial productivity. The water was from gravity filtration samples.
See Niskin Bottle and Cast List EN556 to link specific casts and bottles to each experiment: https://www.bco-dmo.org/dataset/717427 .
The following is the text of the dataset description provided by BCO-DMO:
Gravity filtered polysaccharide hydrolysis rates - EN556
Dataset Description:
This dataset includes polysaccharide hydrolysis rates to measure microbial enzyme activities and bacterial productivity. The water was from gravity filtration samples.
See Niskin Bottle and Cast List EN556 to link specific casts and bottles to each experiment: https://www.bco-dmo.org/dataset/717427 .
Dataset Citation
- Cite as: Arnosti, Carol (2024). Hydrolysis rates from gravity filtered samples, plate reader results from RV/Endeavor EN556, 2015 (Patterns of activities project) (NCEI Accession 0291303). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0291303. Accessed [date].
Dataset Identifiers
ISO 19115-2 Metadata
gov.noaa.nodc:0291303
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Ordering Instructions | Contact NCEI for other distribution options and instructions. |
Distributor |
NOAA National Centers for Environmental Information +1-301-713-3277 NCEI.Info@noaa.gov |
Dataset Point of Contact |
NOAA National Centers for Environmental Information ncei.info@noaa.gov |
Time Period | 2015-04-27 to 2015-05-02 |
Spatial Bounding Box Coordinates |
West: -71.0086
East: -68.4078
South: 37.6675
North: 40.0725
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Data Presentation Form | Digital table - digital representation of facts or figures systematically displayed, especially in columns |
Dataset Progress Status | Complete - production of the data has been completed Historical archive - data has been stored in an offline storage facility |
Data Update Frequency | As needed |
Supplemental Information | Acquisition Description: Seawater was transferred to 20 L carboys that were rinsed three times with water from the sampling depth and then filled with seawater from a single Niskin bottle, using silicone tubing that had been acid washed then rinsed with distilled water prior to use. From each carboy, water was dispensed into smaller glass containers that were cleaned and pre-rinsed three times with water from the carboy prior to dispensing. This water was used to measure cell counts, bacterial productivity, and the activities of polysaccharide hydrolases, peptidases, and glucosidases. A separate glass Duran bottle was filled with seawater from the carboy and sterilized in an autoclave for 20-30 minutes to serve as a killed control for microbial activity measurements. Experiments on (operationally defined) particles were carried out by gravity-filtering water through 3 µm pore size filters. 1/12th sections of the 3 µm pore-size filters were submerged in 15 mL artificial seawater; enzyme activities were measured as described below. Two substrates, -glucose and -glucose linked to a 4-methylumbelliferyl (MUF) fluorophore, were used to measure glucosidase activities. Five substrates linked to a 7-amido-4-methyl coumarin (MCA) fluorophore, one amino acid – leucine – and four oligopeptides – the chymotrypsin substrates alanine-alanine-phenylalanine (AAF) and alanine-alanine-proline-phenylalanine (AAPF), and the trypsin substrates glutamine-alanine-arginine (QAR) and phenylalanine-serine-arginine (FSR) – were used to measure exo- and endo-acting peptidase activities, respectively. Hydrolysis rates of the substrates were measured as an increase in fluorescence as the fluorophore was hydrolyzed from the substrate over time [as in Hoppe, 1993; Obayashi and Suzuki, 2005]. Incubations with the seven low molecular weight substrates were set up in a 96-well plate. For each substrate, triplicate wells were filled with a total volume of 200 L seawater for experimental incubations; triplicate wells were filled with 200 L autoclaved seawater for killed control incubations. Substrate was added at saturating concentrations. A saturation curve was determined with surface water from each station to determine saturating concentrations of substrate. The saturating concentration was identified as the lowest tested concentration of substrate at which additional substrate did not yield higher rates of hydrolysis. Fluorescence was measured over 24-48 hours incubation time with a plate reader (TECAN spectrafluor plus; 360 nm excitation, 460 emission), with timepoints taken every 4-6 hours. Hydrolysis rates were calculated from the rate of increase of fluorescence in the incubation over time relative to a set of standards of known concentration of fluorophore. Scripts to calculate hydrolysis rates and produce the figures shown here are available in the associated Github repository [Hoarfrost, 2017]. |
Purpose | This dataset is available to the public for a wide variety of uses including scientific research and analysis. |
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Platform keywords | NODC PLATFORM NAMES THESAURUS BCO-DMO Platform Names Global Change Master Directory (GCMD) Platform Keywords ICES/SeaDataNet Ship Codes |
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Last Modified: 2024-05-31T15:15:28Z
For questions about the information on this page, please email: ncei.info@noaa.gov
For questions about the information on this page, please email: ncei.info@noaa.gov