Experimental grazing rates of sand dollar larvae (Dendraster excentricus) on algae (Dunaliella tertiolecta) under different ocean acidification conditions, July 2017 (NCEI Accession 0288316)
This dataset contains chemical and physical data collectedat Coastal Pacific, USA from 2017-07-07 to 2017-07-31. These data include pH and water temperature. The instruments used to collect these data include Microscope-Optical. These data were collected by Dr Brady M. Olson, Dr Sylvia Yang, and Shawn M Arellano of Western Washington University as part of the "RUI: Will climate change cause 'lazy larvae'? Effects of climate stressors on larval behavior and dispersal (Climate stressors on larvae)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-09-25.
The following is the text of the dataset description provided by BCO-DMO:
Dendraster_Grazing_OA_Expt2017
Dataset Description:
Experimental grazing rates of sand dollar larvae ( Dendraster excentricus ) on algae ( Dunaliella tertiolecta) under different ocean acidification conditions, July 2017.
The following is the text of the dataset description provided by BCO-DMO:
Dendraster_Grazing_OA_Expt2017
Dataset Description:
Experimental grazing rates of sand dollar larvae ( Dendraster excentricus ) on algae ( Dunaliella tertiolecta) under different ocean acidification conditions, July 2017.
Dataset Citation
- Cite as: Arellano, Shawn M.; Olson, Brady M.; Yang, Sylvia (2024). Experimental grazing rates of sand dollar larvae (Dendraster excentricus) on algae (Dunaliella tertiolecta) under different ocean acidification conditions, July 2017 (NCEI Accession 0288316). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0288316. Accessed [date].
Dataset Identifiers
ISO 19115-2 Metadata
gov.noaa.nodc:0288316
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Ordering Instructions | Contact NCEI for other distribution options and instructions. |
Distributor |
NOAA National Centers for Environmental Information +1-301-713-3277 NCEI.Info@noaa.gov |
Dataset Point of Contact |
NOAA National Centers for Environmental Information ncei.info@noaa.gov |
Coverage Description | Coastal Pacific, USA |
Time Period | 2017-07-07 to 2017-07-31 |
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Dataset Progress Status | Complete - production of the data has been completed Historical archive - data has been stored in an offline storage facility |
Data Update Frequency | As needed |
Supplemental Information | Acquisition Description: Spawning and fertilization We collected adult sand dollars (D. excentricus) from Semiahmoo Bay, WA, on July 7, 2017 and maintained them in 14°C continuous flowing seawater at the Shannon Point Marine Center. On July 12, 2017 we induced twelve individuals to spawn by injecting 1-mL of 0.5-M KCl into the coelom following methods outlined by Strathmann (1987). We then collected and mixed concentrated gametes of four males and four females for fertilization. We added five drops of sperm to 500-mL of filtered seawater and 5-mL of eggs. We placed the fertilized eggs in 12°C incubator and bubbled them with ambient pCO2 condition for 12-hrs before dividing the embryos into pCO2 treatment conditions before gastrulation. We then counted and transferred the larvae into jars with 1.5 L of nanopore filtered seawater at densities of 1-2 individuals mL-1. Grazing experiment To assess the interactive effects of temperature and pCO2 on Dunaliella excentricus feeding behavior, our experimental design had six treatments with four experimental jars (replicates) in each. The treatments combined three levels of CO2: 400 ppmv (ambient atmospheric level), 800 ppmv (moderate atmoshpheric level) and 1,500 ppmv (high atmospheric level), and two temperatures: 12°C (ambient temperature) and 17°C (high temperature). We fed Dunaliella tertiolecta at approximately 6,000 cells ml-1 to six-arm stage larvae to evaluate feeding rates at each treatment condition. For each replicate, a corresponding 150-mL control bottle containing only D. tertiolecta was also prepared. Feeding rate was estimated as ingestion rate by measuring the algal concentration (cells ml-1) at the beginning (T0) and after 24 hours (Tf) in control bottles and experimental jars using a Sedgewick Rafter Chamber (Stumpp et al., 2011). Ingestion rate (cells ind-1 hr-1) was calculated as I = (Clearance rate) x (time-average algae concentration). This dataset includes unprocessed data and simple data calculations accomplished with Excel. |
Purpose | This dataset is available to the public for a wide variety of uses including scientific research and analysis. |
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Last Modified: 2024-05-31T15:15:28Z
For questions about the information on this page, please email: ncei.info@noaa.gov
For questions about the information on this page, please email: ncei.info@noaa.gov