Environmental data collected in marine lakes in Palau in 2010 from small boats (NCEI Accession 0278824)
This dataset contains chemical and physical data collected on Small boats - CRRF during cruise Palau_lakes in the Philippine Sea from 2010-08-21 to 2010-09-03. These data include Ammonium, Nitrate, Nitrite, conductivity, depth, dissolved Oxygen, pH, reactive phosphorus (PO4), salinity calculated from CTD primary sensors, and water temperature. The instruments used to collect these data include Flow Injection Analyzer, GO-FLO Bottle, and Hydrolab Series 5 probes. These data were collected by John Michael Beman and Michael N Dawson of University of California-Merced as part of the "Do Parallel Patterns Arise from Parallel Processes? (PaPaPro)" project and "Dimensions of Biodiversity (Dimensions of Biodiversity)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-08-06.
The following is the text of the dataset description provided by BCO-DMO:
Environmental data collected in marine lakes in Palau in 2010
Dataset Description:
Environmental data collected in marine lakes in Palau in 2010.
The following is the text of the dataset description provided by BCO-DMO:
Environmental data collected in marine lakes in Palau in 2010
Dataset Description:
Environmental data collected in marine lakes in Palau in 2010.
Dataset Citation
- Cite as: Beman, John Michael; Dawson, Michael N. (2023). Environmental data collected in marine lakes in Palau in 2010 from small boats (NCEI Accession 0278824). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0278824. Accessed [date].
Dataset Identifiers
ISO 19115-2 Metadata
gov.noaa.nodc:0278824
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NOAA National Centers for Environmental Information +1-301-713-3277 NCEI.Info@noaa.gov |
Dataset Point of Contact |
NOAA National Centers for Environmental Information ncei.info@noaa.gov |
Time Period | 2010-08-21 to 2010-09-03 |
Spatial Bounding Box Coordinates |
West: 134.269
East: 134.509
South: 7.117
North: 7.323
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Data Presentation Form | Digital table - digital representation of facts or figures systematically displayed, especially in columns |
Dataset Progress Status | Complete - production of the data has been completed Historical archive - data has been stored in an offline storage facility |
Data Update Frequency | As needed |
Supplemental Information | Acquisition Description: Refer to the following paper for complete methodology: Meyerhof, M. et al. 2016. Microbial community diversity, structure and assembly across oxygen gradients in meromictic marine lakes, Palau. Environmental Microbiology. doi: 10.1111/1462-2920.13416 In summary (extracted from above paper): We studied five meromictic lakes in Palau: Spooky Lake (SLM), Goby Lake (GLK), Ongeim’l Tketau Lake (OTM, known colloquially as Jellyfish Lake), Clear Lake (CLM) and Ngermeuangel Lake (NLK). One holomictic lake (Mekeald Lake; MLN) and an ocean site at the German Channel on the southwestern side of the islands (OS-GC) were also sampled for comparison. We vertically profiled dissolved oxygen (DO), temperature, pH, chlorophyll fluorescence and salinity/conductivity using a HydroLab DS5 (Hach Company, Loveland, CO, USA) and sampled three depth layers within each meromictic lake: the mixolimnion (0–5 m depth), the monimolimnion (5–20 m depth) and intermediate depths near the chemocline; these intermediate depths ranged from 1 to 15 m, depending on the depth of the chemocline within the individual lakes. We sampled comparable depths at MLN (5–20 m) and OS-GC (5–30 m). For QPCR analysis of functional genes (dsrA, amoA and nirS) and specific functional groups, as well as analysis dissolved nutrient concentrations, we collected additional samples above and below the chemocline to capture abrupt transitions in biogeochemical conditions across this interface. Samples were collected from small boats using a horizontal, 2.5 L GoFlo bottle (General Oceanics, Miami, FL, USA), transferred to 1 L polycarbonate bottles, and stored in the dark during transit to the Coral Reef Research Foundation laboratory in Koror, Palau. Water samples were filtered using a peristaltic pump and 0.22 um Durapore PVDF hydrophilic filters (Millipore, Billerica, MA, USA). Filters were immediately frozen in 800 uL Sucrose-Tris-EDTA (STE) lysis buffer (750 mM sucrose, 20 mM EDTA, 400 mM NaCl and 50 mM Tris) in 2 mL Lysing Matrix E tubes (MP Biomedicals, Solon, OH, USA), and stored at -20 C until transport to the United States, where they were stored at -80 C until extraction (Dry ice and liquid nitrogen are not readily available in Palau.) During sample filtration, 50 mL of filtrate was collected in high density polyethylene bottles for subsequent nutrient analysis at the University of California, Santa Barbara (UCSB) Marine Analytical Laboratory. Samples were analyzed for ammonium (UCSB MAL analytical method for ammonium, see below; Diamond and Huberty, 1996), nitrite (Environmental Protection Agency (EPA) Method 353.2; Schroeder, 1997), nitrite+nitrate (EPA Method 353.2; Diamond, 1997) and phosphate (EPA Method 365.1; Huberty and Diamond, 1998), on a Lachat QuikChem 8000 Flow Injection Analyzer (Hach Company, Loveland, CO, USA). A handful of samples containing large concentrations of sulfide were not analyzed for nitrate, as sulfide damages the cadmium reduction column. For ammonium analysis, each sample was injected into a flowing carrier stream through an injection valve, and then merged with an alkaline solution stream; the produced ammonia was diffused through a hydrophobic, gas-permeable membrane into a recipient stream containing a pH indicator. Colour change occurs in the indicator solution due to an increase in pH, and the concentration of ammonia was determined spectrophotometrically based on absorption at 570 nm. For all analyses, a mid-range check standard bracketed every 20 samples to verify the accuracy of the measurements, and samples that were detected outside of the standards’ range were diluted 1:10 and reanalyzed. Detection limits were 0.10 uM for phosphate, 0.10 uM for nitrite, 0.20 uM for nitrite+nitrate and 0.10 uM for ammonium. |
Purpose | This dataset is available to the public for a wide variety of uses including scientific research and analysis. |
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Last Modified: 2024-05-31T15:15:28Z
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For questions about the information on this page, please email: ncei.info@noaa.gov