Treatment description and growth parameters for Thalassiosira weissflogii generating d13C-AA, collected on R/V Meteor M77 in the Peruvian ocean margin from November to December 2008 (NCEI Accession 0278787)
This dataset contains chemical, optical, and physical data collected on R/V Meteor during cruise M77 at Peruvian ocean margin from 2008-11-01 to 2008-12-31. These data include irradiance, pH, and salinity calculated from CTD primary sensors. The instruments used to collect these data include Light Meter, Salinity Sensor, Water Temperature Sensor, and pH Sensor. These data were collected by Dr Matthew D. McCarthy of University of California-Santa Cruz as part of the "The Use of Nitrogen Isotopes of Amino Acids To Understand Marine Sedimentary 15N Records (Amino Acid Sediment 15N)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-03-19.
The following is the text of the dataset description provided by BCO-DMO:
Algal treatment data and ID abbreviations
Dataset Description:
Treatment description and growth parameters for Thalassiosira weissflogii generating d13C-AA.
Temperature and irradiance values are means of outdoor conditions; the 25% and 75% quartiles for temperature were 9.8 and 11.3 deg C, and the 25% and 75% quartiles for irradiance levels were 269 and 1274 umol m-2 s-1.
These data were published in:
Larsen T, , L. T. Bach, R. Salvatteci, M. Ventura, Y. V. Wang, N. Andersen, and M. D. McCarthy (2015). Assessing the potential of amino acid d13C patterns as a carbon source tracer in marine sediments: effects of algal growth conditions and sedimentary diagenesis. (Biogeosciences, 12, 4979-4992. https://doi.org/10.5194/bg-12-4979-2015
The following is the text of the dataset description provided by BCO-DMO:
Algal treatment data and ID abbreviations
Dataset Description:
Treatment description and growth parameters for Thalassiosira weissflogii generating d13C-AA.
Temperature and irradiance values are means of outdoor conditions; the 25% and 75% quartiles for temperature were 9.8 and 11.3 deg C, and the 25% and 75% quartiles for irradiance levels were 269 and 1274 umol m-2 s-1.
These data were published in:
Larsen T, , L. T. Bach, R. Salvatteci, M. Ventura, Y. V. Wang, N. Andersen, and M. D. McCarthy (2015). Assessing the potential of amino acid d13C patterns as a carbon source tracer in marine sediments: effects of algal growth conditions and sedimentary diagenesis. (Biogeosciences, 12, 4979-4992. https://doi.org/10.5194/bg-12-4979-2015
Dataset Citation
- Cite as: McCarthy, Matthew D. (2023). Treatment description and growth parameters for Thalassiosira weissflogii generating d13C-AA, collected on R/V Meteor M77 in the Peruvian ocean margin from November to December 2008 (NCEI Accession 0278787). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0278787. Accessed [date].
Dataset Identifiers
ISO 19115-2 Metadata
gov.noaa.nodc:0278787
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NOAA National Centers for Environmental Information +1-301-713-3277 NCEI.Info@noaa.gov |
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Coverage Description | Peruvian ocean margin |
Time Period | 2008-11-01 to 2008-12-31 |
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Dataset Progress Status | Complete - production of the data has been completed Historical archive - data has been stored in an offline storage facility |
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Supplemental Information | Acquisition Description: Culturing The marine diatom Thalassiosira weissflogii Grunow (strain CCMP 1010) was cultured in sterile filtered natural North Sea water (Schleswig-Holstein, Germany) or Baltic Sea water (Schleswig-Holstein, Germany). The medium was enriched with f/4 concentrations of macro- and micronutrients (nitrate, phosphate, silicic acid, trace metal mixture, vitamin mixture (Guillard and Ryther, 1962). All experiments were performed in sterile 2.1 L Schott Duran glass bottles. These bottles were made of borosilicate glass (filters UV radiation <310 nm) except for the quartz glass bottles (pure silica without UV radiation filter) used in the UV experiment. The cultures were either incubated in climate chambers with 400 –700 nm radiation or 10 cm below water level at low tide in Kiel Fjord in May 2011. Water temperature and light irradiance data were obtained from the weather station maintained by the GEOMAR institute in Kiel, Germany. Growth conditions for the various treatments, i.e. salinity, pH, temperature, and irradiance are given in Table 1. pH values (reported on free scale) were measured with separate glass and reference electrodes (Metrohm) and calculated with equation 3 from DOE 2007 chapter 6b (Dickson et al., 2007) as described in (Bach et al., 2012). Cultures were inoculated with densities of 20 cells ml-1. Cell densities and equivalent spherical diameters were determined with a Coulter Counter (Beckman Coulter) at the beginning and the end of the experiment, respectively. When incubations ended, cells were filtered on 47 mm diameter, 5 um mesh size Nucleopore Track-Etch Membrane filters (Whatman) and frozen at -18 deg C immediately after filtration. |
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Last Modified: 2024-05-31T15:15:28Z
For questions about the information on this page, please email: ncei.info@noaa.gov
For questions about the information on this page, please email: ncei.info@noaa.gov