Dilution batch-culture bioassay (remineralization) experiments from SBDOM11 project cruise from R/V Point Sur PS1103 in the Santa Barbara Channel, May 2011 (SBDOM project, SBC LTER project) (NCEI Accession 0278759)
This dataset contains biological and chemical data collected on R/V Point Sur during cruise PS1103 from 2011-05-11 to 2011-07-24. These data include dissolved organic Carbon and growth. The instruments used to collect these data include Automated DNA Sequencer, Flow Cytometer, PCR Thermal Cycler, and Shimadzu TOC-V Analyzer. These data were collected by Dr Craig Carlson and Mark Brzezinski of University of California-Santa Barbara as part of the "Mechanisms controlling the production and fate of DOM during diatom blooms (SBDOM)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-08-28.
The following is the text of the dataset description provided by BCO-DMO:
bacterial abundance, growth rates, and DOC from SBDOM11 cruise
Dataset Description:
This dataset includes results from dilution batch-culture bioassay experiments reporting bacterial abundance, growth rates, barcode sequences and DOC were from the SBDOM 11 cruise in May 2011.
The following is the text of the dataset description provided by BCO-DMO:
bacterial abundance, growth rates, and DOC from SBDOM11 cruise
Dataset Description:
This dataset includes results from dilution batch-culture bioassay experiments reporting bacterial abundance, growth rates, barcode sequences and DOC were from the SBDOM 11 cruise in May 2011.
Dataset Citation
- Cite as: Carlson, Craig; Brzezinski, Mark A. (2023). Dilution batch-culture bioassay (remineralization) experiments from SBDOM11 project cruise from R/V Point Sur PS1103 in the Santa Barbara Channel, May 2011 (SBDOM project, SBC LTER project) (NCEI Accession 0278759). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0278759. Accessed [date].
Dataset Identifiers
ISO 19115-2 Metadata
gov.noaa.nodc:0278759
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Ordering Instructions | Contact NCEI for other distribution options and instructions. |
Distributor |
NOAA National Centers for Environmental Information +1-301-713-3277 NCEI.Info@noaa.gov |
Dataset Point of Contact |
NOAA National Centers for Environmental Information ncei.info@noaa.gov |
Time Period | 2011-05-11 to 2011-07-24 |
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West: -120.2
East: -120.2
South: 34.3
North: 34.3
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Data Presentation Form | Digital table - digital representation of facts or figures systematically displayed, especially in columns |
Dataset Progress Status | Complete - production of the data has been completed Historical archive - data has been stored in an offline storage facility |
Data Update Frequency | As needed |
Supplemental Information | Acquisition Description: Dilution batch-culture bioassay experiments were established 11 May 2011 - 15 May 2011 on the SBDOM 11 cruise using water samples collected from the upper 8m of the water column. Water was gravity-filtered from the sampling Niskin bottle through two mixed cellulose ester filters (1.2 µm and 0.2 µm). Duplicate 500 mL polycarbonate bottles were filled with 375 mL 0.2 µm filtrate (filter sterilized media) and 125 mL 1.2 µm filtrate (bacterial inoculum) collected from the same cast. Bottles were incubated in the dark at 11degrees C for 10 weeks. Samples for bacterial abundance, DOM, and community composition by DNA were collected by decanting from the bottle at specified intervals. DOC was quantified by high-temperature combustion on a modified Shimadzu TOC-V. We used 1.5 µM DOC as a detection limit for these experiments. Bacterial abundance was measured using a BD LSRII flow cytometer with an autosampler attachment. Samples were preserved in sterile cryovials with 0.2% final concentration paraformaldehyde, then stored frozen until analysis. Samples were stained with SYBR Green I for enumeration. DNA was collected from duplicate bottles combined (to conserve volume); 125 mL from each bottle was vacuum-filtered onto a 0.2 µm polyethersulfone filter and frozen. Samples were lysed in sucrose lysis buffer with 1% w/v sodium dodecyl sulfate and 0.2 mg mL-1 proteinase-K, and genomic DNA was extracted using a Qiagen DNEasy silica centrifugation kit. DNA was used to conduct multiplex amplicon pyrosequencing using primers 8F and 338R, following the protocol of Nelson et al. (2014; doi: 10.1111/1462-2920.12241). Sequence analysis was conducted in mothur (Schloss et al. 2009; doi: 10.1128/AEM.01541-09) using a non-redundant subset of the SILVA SSU Ref 16S alignment database (v111) curated as in Nelson et al. (2014). |
Purpose | This dataset is available to the public for a wide variety of uses including scientific research and analysis. |
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Data Center keywords | NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS Global Change Master Directory (GCMD) Data Center Keywords |
Platform keywords | NODC PLATFORM NAMES THESAURUS BCO-DMO Platform Names Global Change Master Directory (GCMD) Platform Keywords ICES/SeaDataNet Ship Codes |
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Project keywords | BCO-DMO Standard Projects Provider Cruise IDs Provider Funding Award Information |
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Last Modified: 2024-05-31T15:15:28Z
For questions about the information on this page, please email: ncei.info@noaa.gov
For questions about the information on this page, please email: ncei.info@noaa.gov