Cellular trace elements collected on cruise TN280 (GeoMICS project) along Line P in the NE Pacific in May 2012 (NCEI Accession 0278711)

This dataset contains chemical and physical data collected on R/V Thomas G. Thompson during cruise TN280 in the North Pacific Ocean on 2021-02-24. These data include Carbon, Iron, Manganese, Silicon, and depth. The instruments used to collect these data include GO-FLO Bottle, Trace Metal Bottle, and X-ray fluorescence analyzer. These data were collected by Benjamin Twining of Bigelow Laboratory for Ocean Sciences as part of the "Characterizing biological function across a persistent oceanographic "hotspot" in the NE Pacific Ocean (GeoMICS Bio)" and "The relationship between microbial biogeography and ocean chemistry across a persistent oceanographic "hot spot" in the NE Pacific Ocean (GeoMICS Chem)" projects and "GeoMICS (GeoMICS)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2021-05-18.

The following is the text of the dataset description provided by BCO-DMO:

Cellular trace elements from TN280

Dataset Description:
Acquisition Description:
Samples were collected with trace-metal clean rosette and GO-FLO bottles. A small aliquot of unfiltered seawater was collected following protocols described in Twining et al. (2015). Cellular metals were analyzed with the 2-ID-E microprobe beamline at the Advanced Photon Source, Argonne National Laboratory. Incident beam energy was 10 keV to enable the excitation of K α fluorescence for elements ranging in atomic number from Si (14) to Zn (30). Element quantification was performed by averaging the spectra from pixels representing the cells of interest. Spectra were also extracted from a background area close to each cell. The spectra were then fit with MAPS, a custom fitting software package (Vogt, 2003). Concentrations were calculated based on conversion factors obtained by running the thin-film standards NBS 1832, NBS 1833, and custom Si, P, and Fe standards made by Micromatter XRF. Cell volume was calculated based on measurements taken from bright field images of the cells and using the equations of Hillebrand et al. (1999). Cellular C was then calculated from the volumes using the equations described in Menden-Deuer and Lessard (2000).

Complete methodology is published in Twining et al. (2020).