Respirometry data for pelagic crustaceans, cephalopods, and fish collected on R/V Sikuliaq cruise SKQ201701S from January to February 2017 (NCEI Accession 0278540)

This dataset contains biological, physical, and survey - biological data collected on R/V Sikuliaq during cruise SKQ201701S from 2017-01-22 to 2017-02-13. These data include species, taxon, and water temperature. The instruments used to collect these data include Dissolved Oxygen Sensor, MOCNESS1, Tucker Trawl, and plate reader. These data were collected by Christopher Roman and Karen Wishner of University of Rhode Island and Brad Seibel of University of South Florida as part of the "Collaborative Research: A metabolic index to predict the consequences of climate change for midwater ecosystems (Metabolic Index)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2021-07-22.

The following is the text of the dataset description provided by BCO-DMO:

Respirometry data for pelagic crustaceans, cephalopods, and fish

Dataset Description:
Acquisition Description:
This dataset comprises results of respirometry experiments conducted at sea. Experimental animals were collected using either a modified tucker trawl or MOCNESS. Metabolic rates and critical oxygen pressures were measured using published methods (Birk et al., 2018; 2019; Wishner et al., 2018; Seibel et al., 2021). Briefly, individual specimens were placed in darkened sealed chambers filled with 0.2 μm filtered seawater that had been treated with antibiotics (25 mg/L each of streptomycin and actinomycin) to minimize bacterial respiration. The ratio of chamber size to body mass was approximately 1:20. Oxygen content was continuously monitored using Pyroscience Firesting or Presens oxygen meters and fiber optic probes with oxygen-sensitive fluorescent spots. Oxygen meters were calibrated with air-saturated seawater and concentrated Na2SO3 solution (PO2 = 0). Chamber water was mixed with magnetic stirrers (Cole-Parmer Immersible Stirrer EW-04636-50) to ensure homogenous oxygen levels throughout the chamber. Oxygen consumption rates (MO2) were calculated from the slope of PO2 over time. Only linear regressions with an R2 > 0.8 were included. The median MO2 value from non-hypoxic oxygen levels was calculated for each individual. The Pcrit for each individual was calculated using three metrics, including the traditional "breakpoint" method, the "sub-prediction interval (sub-PI)" and the alpha-method.