Accession numbers for metagenome-assembled genomes (MAGs) from samples collected in 2018 on R/V Thompson cruise TN350 at the Upper and Lower Cones, Upper Caldera Wall and NW Caldera, Brothers Volcano (NCEI Accession 0278511)

This dataset contains biological data collected on R/V Thomas G. Thompson during cruise TN350 in the South Pacific Ocean from 2018-03-01 to 2018-03-31. These data include depth and taxon. The instruments used to collect these data include Automated DNA Sequencer. These data were collected by Anna-Louise Reysenbach of Portland State University and Susan Humphris of Woods Hole Oceanographic Institution as part of the "Collaborative Research: Hydrothermal and Microbiological Investigations of the Active Brothers Volcano in the Kermadec Arc (Brothers Volcano Microbiology)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2022-08-24.

The following is the text of the dataset description provided by BCO-DMO:

Brothers Volcano Metagenome-Assembled Genome Accession Numbers

Dataset Description:
Acquisition Description:
Water samples and hydrothermal vent deposits were collected as described in Reysenbach & St. John et al., 2020. Samples were collected with remotely operated vehicle ROV Jason. Water samples were obtained using major water samplers. Hydrothermal deposits were placed in sealed bioboxes in the ROV sampling basket to restrict flushing of samples by surrounding seawater during the ascent. Once shipboard, 1L of water samples were filtered through a 0.2-μm Sterivex filter (Merck). For the hydrothermal deposits, the outer few millimeters (up to approximately 5 mm) was subsampled for DNA extraction. These exterior samples were homogenized and stored in cryovials at -80 degrees C for subsequent DNA extraction. For the water samples, the DNA was extracted from the sterivex filters as previously described by R. E. Anderson et al. (2017). The DNA was extracted from the homogenized hydrothermal depot samples using the DNeasy PowerSoil kit (Qiagen) All purified environmental DNA was stored at -80 degrees C. After DNA extraction and library preparation, metagenomes were sequenced using the Illumina HiSeq 3000.