Experimental data on growth rates of Pleurochrysis carterae analyzed at Bigelow Laboratory from 2013. (OA Copes Coccoliths project) (NCEI Accession 0278471)
This dataset contains biological data collected at lab Bigelow during deployment Balch_2013 at Bigelow Laboratory from 2013-01-01 to 2013-12-31. These data include growth. The instruments used to collect these data include Automated Cell Counter, CO2 Adsorber, and Mass Flow Controller. These data were collected by Dr David Fields and Dr William M. Balch of Bigelow Laboratory for Ocean Sciences as part of the "Effects of ocean acidification on Emiliania huxleyi and Calanus finmarchicus; insights into the oceanic alkalinity and biological carbon pumps (OA_Copes_Coccoliths)" project and "Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA) (SEES-OA)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-04-24.
The following is the text of the dataset description provided by BCO-DMO:
Experimental data on growth rates of Pleurochrysis carterae
Dataset Description:
Experimental results describing the growth rate of Pleurochrysis carterae (NCMA strain 645). It was isolated from 41.525 degrees North, 70.6736 degrees West (Woods Hole, Massachusetts USA), but has been maintained in culture since 1958.
The following is the text of the dataset description provided by BCO-DMO:
Experimental data on growth rates of Pleurochrysis carterae
Dataset Description:
Experimental results describing the growth rate of Pleurochrysis carterae (NCMA strain 645). It was isolated from 41.525 degrees North, 70.6736 degrees West (Woods Hole, Massachusetts USA), but has been maintained in culture since 1958.
Dataset Citation
- Cite as: Balch, William M.; Fields, David (2023). Experimental data on growth rates of Pleurochrysis carterae analyzed at Bigelow Laboratory from 2013. (OA Copes Coccoliths project) (NCEI Accession 0278471). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0278471. Accessed [date].
Dataset Identifiers
ISO 19115-2 Metadata
gov.noaa.nodc:0278471
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NOAA National Centers for Environmental Information +1-301-713-3277 NCEI.Info@noaa.gov |
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NOAA National Centers for Environmental Information ncei.info@noaa.gov |
Coverage Description | Bigelow Laboratory |
Time Period | 2013-01-01 to 2013-12-31 |
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Dataset Progress Status | Complete - production of the data has been completed Historical archive - data has been stored in an offline storage facility |
Data Update Frequency | As needed |
Supplemental Information | Acquisition Description: Cultures: Pleurochrysis carterae cultures were maintained in exponential growth phase under axenic conditions in semi-continuous batch culture using L1-Si media prepared on 0.2 um-filtered, UV-sterilized, autoclaved seawater. Cultures were acclimated to one of three pCO2 treatments for > 9 generations before experiments were performed. Cultures were maintained in an incubator at 16.5 +/- 0.5 degrees C and 470 umol photons/m-2/s PAR on a 14-10 light-dark cycle. pCO2 Treatments: Carbonate chemistry was manipulated by bubbling cultures and prepared media with 500 mL/min with 0.2 um-filtered 280, 380, or 750 ppm pCO2 air. The pCO2 levels of the treatment air were established using two mass flow controllers (Aalborg, Orangeburg, NY, USA) for each treatment to precisely mix in-house compressed air and pure CO2 (Maine Oxy, Auburn, ME, USA). The in-house compressed air was stripped of CO2 to less than 10 ppm CO2 using a Puregas VCD CO2 Adsorber (Puregas, LLC, Broomfield, CO, USA). The pCO2 of the gas mixtures was stable to +/- 8 ppm. pCO2 values of the cultures may be different than the target levels due to biological activity. Growth rate measurements: At the same time each day, the cell density of each pCO2 treatment culture was measured in order to calculate the growth rate. The data analyzed represent three consecutive growth cycles. Cell density: Culture density was measured using a Moxi Z mini automated cell counter (ORFLO Technologies, Ketchum, ID, USA), which has a coefficient of variation of 4%. |
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Last Modified: 2024-05-31T18:50:46Z
For questions about the information on this page, please email: ncei.info@noaa.gov
For questions about the information on this page, please email: ncei.info@noaa.gov