Marine lakes of Palau barcoded specimens from transect survey with both lab identification number (M0D#) and original tube number from 2011-06-04 to 2015-07-02 (NCEI Accession 0278386)
This dataset contains biological and survey - biological data collected on Small boats - CRRF during cruise Palau_lakes from 2011-06-04 to 2015-07-02. These data include phylum. These data were collected by Michael N Dawson of University of California-Merced as part of the "Do Parallel Patterns Arise from Parallel Processes? (PaPaPro)" project and "Dimensions of Biodiversity (Dimensions of Biodiversity)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-07-08.
The following is the text of the dataset description provided by BCO-DMO:
Palau lakes: tissue archive
Dataset Description:
Marine lakes of Palau barcoded specimens from transect survey with both lab identification number (M0D#) and original tube number. The purpose of this dataset is (1) to link unique identification numbers used in different circumstances and files, specifically tube ID used in the field and the M0D# identifier for the lab’s permanent collection, and (2) to summarize the tissue samples collected during biodiversity surveys under this project.
* NOTE: The P.I.'s are using this dataset to write papers. Please contact them before using these data to make sure you are not duplicating efforts.
The following is the text of the dataset description provided by BCO-DMO:
Palau lakes: tissue archive
Dataset Description:
Marine lakes of Palau barcoded specimens from transect survey with both lab identification number (M0D#) and original tube number. The purpose of this dataset is (1) to link unique identification numbers used in different circumstances and files, specifically tube ID used in the field and the M0D# identifier for the lab’s permanent collection, and (2) to summarize the tissue samples collected during biodiversity surveys under this project.
* NOTE: The P.I.'s are using this dataset to write papers. Please contact them before using these data to make sure you are not duplicating efforts.
Dataset Citation
- Cite as: Dawson, Michael N. (2023). Marine lakes of Palau barcoded specimens from transect survey with both lab identification number (M0D#) and original tube number from 2011-06-04 to 2015-07-02 (NCEI Accession 0278386). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0278386. Accessed [date].
Dataset Identifiers
ISO 19115-2 Metadata
gov.noaa.nodc:0278386
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Ordering Instructions | Contact NCEI for other distribution options and instructions. |
Distributor |
NOAA National Centers for Environmental Information +1-301-713-3277 NCEI.Info@noaa.gov |
Dataset Point of Contact |
NOAA National Centers for Environmental Information ncei.info@noaa.gov |
Time Period | 2011-06-04 to 2015-07-02 |
Spatial Bounding Box Coordinates |
West: 134.3447
East: 134.5089
South: 7.1506
North: 7.3237
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Data Presentation Form | Digital table - digital representation of facts or figures systematically displayed, especially in columns |
Dataset Progress Status | Complete - production of the data has been completed Historical archive - data has been stored in an offline storage facility |
Data Update Frequency | As needed |
Supplemental Information | Acquisition Description: Sample collection: Each lake was sampled using the point intercept transect method at no less than 10 randomly chosen sites around its perimeter (unless the small size of a lake precluded this number of non-overlapping sites). At each site, three parallel transects approximately were run 5 m apart from the intertidal (0 m) to the deepest depth accessible to SCUBA divers (i.e. the bottom of the lake, or bottom of the epilimnion, or the divers’ maximum certified depth). In lakes 8m or deeper, a line (‘the horizontal’) was placed, at eight evenly spaced target depths (1–4 m depth intervals, depending on lake), orthogonal to each of the transect lines so that small (2.0 cm diameter) cells fell over four points A–D each at 15 cm increments to the right of the transect line at the same depth. At each depth, from deepest to shallowest, the actual depth was measured in feet with a dive computer, the ‘horizontal’ was photographed from ~0.5 m distance, the substrate type was recorded, and then each cell photographed in close-up. A tissue sample of the ‘primary’ organism within each cell, i.e. the organism at the center of the cell, or if no organism in the centre then the first organisms at the periphery going clockwise from noon, or if only sediment visible, the organism within the sediment directly under the Cell was then biopsied for DNA analyses and placed in a container labeled with site, depth, and cell code A–D. Because the benthos may be three dimensional a ‘primary’ organism might also have many ‘secondary’ epibionts and/or epiphytes attached. Any organisms in the photographs but not sampled were classified as ‘tertiary’. After all four cells were sampled at a depth, the diver ascended to the next shallowest depth on his/her transect and repeated the procedure. Thus, at each randomly chosen site, we surveyed a total of 96 points from the deepest to shallowest depths of the lake habitable by macro-invertebrates and macrophytes, with two categories of exception. (1) If a lake was <8 m deep, the number of depths sampled was equivalent to the maximum depth in meters. (2) Lakes with gently sloping sides could lead to adjacent target depths being >10 m apart leading to undersampling of horizontal patchiness; in which case the transect distance between adjacent target depths was estimated and divided in half or in thirds so that no two samples were more than 10 transect meters apart. At the surface, at the end of each dive, samples were transferred to individual tubes of 95% ethanol labeled with a field number composed of lake, site, collector, depth, and cell IDs. Each evening, new samples were stored in a freezer, dive profiles were downloaded, and fieldnotes were transcribed to a standardized electronic data sheet. Error-checking biodiversity transect files Each evening, or as soon thereafter as possible, divers compared specimens to standardize field-identifications and all tissue samples were reconciled to the electronic data sheet for each lake using tube labels, original field notes, photographs of specimens in the field, and visual inspection of tube contents. If necessary, primary and secondary specimens were placed in individually labelled tubes of ethanol. In cases of discrepancy between electronic notes and original field notes, we edited the electronic data sheet to be consistent with original notes and corroborated this by double-checking the original photographs and inspecting tube contents. Significant changes—i.e. samples that could not be reconciled after accounting for tube transpositions, mislabeling, or mis-identification in the field—were logged in a separate file highlighting the specific change and justification. If a specimen was unable to be reconciled with notes it was discarded (this was necessary for only one specimen). Subsequently, every tissue sample was assigned an unique identifying number (M0D#) for curation; during this process, every tenth sample was double-checked for agreement between the original field number and new M0D#. |
Purpose | This dataset is available to the public for a wide variety of uses including scientific research and analysis. |
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Data Center keywords | NODC SUBMITTING INSTITUTION NAMES THESAURUS Global Change Master Directory (GCMD) Data Center Keywords |
Platform keywords | BCO-DMO Platform Names Global Change Master Directory (GCMD) Platform Keywords |
Place keywords | Provider Place Names |
Project keywords | BCO-DMO Standard Programs BCO-DMO Standard Projects Provider Cruise IDs Provider Funding Award Information |
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Last Modified: 2024-05-31T15:15:28Z
For questions about the information on this page, please email: ncei.info@noaa.gov
For questions about the information on this page, please email: ncei.info@noaa.gov