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Clones of Prochlorococcus MIT9312 mixed with clonal isolates of the heterotrophic "helper" bacterium Alteromonas EZ55 (P-ExpEv project) from 2015-12-01 to 2015-12-31 (NCEI Accession 0278354)

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This dataset contains biological, chemical, and physical data collected at LDEO during deployment Dyhrman_2013 at Dyhrman lab, LDEO from 2015-12-01 to 2015-12-31. These data include PAR_photons, Partial pressure of CO2, depth, dissolved inorganic Carbon, pH, taxon, total alkalinity (TA), and water temperature. The instruments used to collect these data include Automated DNA Sequencer. These data were collected by Sonya T. Dyhrman of Lamont-Doherty Earth Observatory and James Jeffrey Morris of University of Alabama at Birmingham as part of the "Impacts of Evolution on the Response of Phytoplankton Populations to Rising CO2 (P-ExpEv)" project and "Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA) (SEES-OA)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2021-05-28.

The following is the text of the dataset description provided by BCO-DMO:

Clones of Prochlorococcus MIT9312 and link to BioProject

Dataset Description:
This dataset includes a link to Prochlorococcus clone NCBI BioProject and associated metadata.
PRJNA377729 : https://www.ncbi.nlm.nih.gov/bioproject/PRJNA377729

Dataset Citation

Cite as: Dyhrman, Sonya T.; Morris, James Jeffrey (2023). Clones of Prochlorococcus MIT9312 mixed with clonal isolates of the heterotrophic "helper" bacterium Alteromonas EZ55 (P-ExpEv project) from 2015-12-01 to 2015-12-31 (NCEI Accession 0278354). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0278354. Accessed [date].

Dataset Identifiers

ISO 19115-2 Metadata

gov.noaa.nodc:0278354

Full Text · XML

Download Data	HTTPS (download) Navigate directly to the URL for data access and direct download. FTP (download) These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP).
Distribution Formats	TSV
Ordering Instructions	Contact NCEI for other distribution options and instructions.
Distributor	NOAA National Centers for Environmental Information +1-301-713-3277 NCEI.Info@noaa.gov
Dataset Point of Contact	NOAA National Centers for Environmental Information ncei.info@noaa.gov

Coverage Description	Dyhrman lab, LDEO
Time Period	2015-12-01 to 2015-12-31
Spatial Bounding Box Coordinates	West: East: South: North:
Spatial Coverage Map

General Documentation

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Descriptive Information
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Associated Resources

Biological, chemical, physical, biogeochemical, ecological, environmental and other data collected from around the world during historical and contemporary periods of biological and chemical oceanographic exploration and research managed and submitted by the Biological and Chemical Oceanography Data Management Office (BCO-DMO)
- NCEI Collection
  Navigate directly to the URL for data access and direct download.
Dyhrman, S. T., Morris, J. J. (2017) Clones of Prochlorococcus MIT9312 mixed with clonal isolates of the heterotrophic "helper" bacterium Alteromonas EZ55 (P-ExpEv project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2017-04-24. https://doi.org/10.26008/1912/bco-dmo.688162.1
- https://doi.org/10.26008/1912/bco-dmo.688162.1 (download)
  originator dataset

gov.noaa.nodc:BCO-DMO

Publication Dates	publication: 2023-05-17
Data Presentation Form	Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status	Complete - production of the data has been completed Historical archive - data has been stored in an offline storage facility
Data Update Frequency	As needed
Supplemental Information	Acquisition Description: Six clones of high light II Prochlorococcus VOL4, a streptomycin-resistant derivative of strain MIT9312 were isolated by dilution to extinction in Pro99 media pretreated with the helper bacterium Alteromonas sp. strain EZ55. Prochlorococcus clones were made axenic by addition of streptomycin. For co-culture experiments, Alteromonas bacteria were introduced to Prochlorococcus cultures prior to growth experiments. Alteromonas was diluted onto YTSS agar plates and each Prochlorococcus culture was inoculated with a single separate colony. Alteromonas stock cultures were grown in YTSS liquid medium and cryopreserved in 20% glycerol at -80 C. Each of the six Prochlorococcus clones was grown in ambient or elevated CO2 in co-culture with Alteromonas, in PEv media. The mRNA libraries were prepared for Illumina Hi-seq 2500 paired-end sequencing (PE100) with TruSeq RNA sample Prep Kit v2 (Illumina, San Diego, CA. Samples were barcoded for multiplex sequencing and run on in a single lane by the Columbia University Genome Center (CUGC) (New York, NY). RNA extractions were performed with the RNeasy Mini Kit.
Purpose	This dataset is available to the public for a wide variety of uses including scientific research and analysis.
Use Limitations	accessLevel: Public Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.

Dataset Citation	Cite as: Dyhrman, Sonya T.; Morris, James Jeffrey (2023). Clones of Prochlorococcus MIT9312 mixed with clonal isolates of the heterotrophic "helper" bacterium Alteromonas EZ55 (P-ExpEv project) from 2015-12-01 to 2015-12-31 (NCEI Accession 0278354). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0278354. Accessed [date].
Cited Authors	Dyhrman, Sonya T. Lamont-Doherty Earth Observatory Morris, James Jeffrey University of Alabama at Birmingham
Principal Investigators	Sonya T. Dyhrman Columbia University; Lamont-Doherty Earth Observatory (LDEO) James Jeffrey Morris University of Alabama - Birmingham (UAB)
Contributors	Columbia University; Lamont-Doherty Earth Observatory (LDEO)
Resource Providers	Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Points of Contact	Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Publishers	NOAA National Centers for Environmental Information
Acknowledgments	Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1314336 Award URL: http://www.nsf.gov/awardsearch/showAward?AWD_ID=1314336

Theme keywords	NODC DATA TYPES THESAURUS DEPTH - OBSERVATION DISSOLVED INORGANIC CARBON (DIC) partial pressure of carbon dioxide - water pH PHOTOSYNTHETIC ACTIVE RADIATION (PAR) TAXONOMIC CODE total alkalinity WATER TEMPERATURE NODC OBSERVATION TYPES THESAURUS biological chemical physical WMO_CategoryCode oceanography BCO-DMO Standard Parameters PAR_photons Partial pressure of CO2 accession number count date depth dissolved inorganic Carbon external_link laboratory latitude no standard parameter pH region sample description sample identification sample type site description taxon total alkalinity (TA) water temperature Global Change Master Directory (GCMD) Science Keywords EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > ALKALINITY EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > CARBON DIOXIDE EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > INORGANIC CARBON EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PH EARTH SCIENCE > OCEANS > OCEAN OPTICS > PHOTOSYNTHETICALLY ACTIVE RADIATION EARTH SCIENCE > OCEANS > OCEAN TEMPERATURE > WATER TEMPERATURE Originator Parameter Names Alkalinity BioProject_link CO2 DIC Media NCBI_BioProject biomaterial_provider co_cultured_with collected_by collection_date depth env_biome genotype geo_loc_name isolate isolation_source lat_lon light_dark_hr light_level organism pH passage_history samp_size sample sample_title sample_type strain temp_C
Data Center keywords	NODC COLLECTING INSTITUTION NAMES THESAURUS Columbia University; Lamont-Doherty Earth Observatory NODC SUBMITTING INSTITUTION NAMES THESAURUS Biological and Chemical Oceanography Data Management Office Global Change Master Directory (GCMD) Data Center Keywords BCO-DMO > Biological and Chemical Oceanography Data Management Office COLUMBIA/LDEO > Lamont-Doherty Earth Observatory, Columbia University
Platform keywords	BCO-DMO Platform Names LDEO Global Change Master Directory (GCMD) Platform Keywords LABORATORY > LABORATORY
Instrument keywords	BCO-DMO Standard Instruments Automated DNA Sequencer Originator Instrument Names Illumina Hi-seq 2500 paired-end sequencing (PE100) with TruSeq RNA sample Prep Kit v2 (Illumina, San Diego, CA)
Place keywords	Provider Place Names Dyhrman lab, LDEO
Project keywords	BCO-DMO Standard Programs Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA) (SEES-OA) BCO-DMO Standard Projects Impacts of Evolution on the Response of Phytoplankton Populations to Rising CO2 (P-ExpEv) Provider Deployment IDs Dyhrman_2013 Provider Funding Award Information Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1314336
Keywords	NCEI ACCESSION NUMBER 0278354

Use Constraints	Cite as: Dyhrman, Sonya T.; Morris, James Jeffrey (2023). Clones of Prochlorococcus MIT9312 mixed with clonal isolates of the heterotrophic "helper" bacterium Alteromonas EZ55 (P-ExpEv project) from 2015-12-01 to 2015-12-31 (NCEI Accession 0278354). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0278354. Accessed [date].
Data License	This dataset is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License. SPDX License: Creative Commons Attribution 4.0 International (CC-BY-4.0)
Access Constraints	Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
Fees	In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.

Lineage information for: dataset
Processing Steps	2023-05-17T13:06:28Z - NCEI Accession 0278354 v1.1 was published.
Output Datasets	NCEI Accession 0278354 v1.1 NCEI Accession 0278354 v1.1 (download) published 2023-05-17T13:06:28Z

Last Modified: 2024-06-26T19:24:12Z
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Clones of Prochlorococcus MIT9312 mixed with clonal isolates of the heterotrophic "helper" bacterium Alteromonas EZ55 (P-ExpEv project) from 2015-12-01 to 2015-12-31 (NCEI Accession 0278354)

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