Coral colony genetic sequence accession numbers for samples collected from the lagoon environment of Ofu Island, American Samoa between 2011 and 2015 (NCEI Accession 0278347)
This dataset contains biological and survey - biological data collected at American_Samoa during deployment Palumbi_AmSamoa_2013-2015 in the South Pacific Ocean from 2011-04-01 to 2015-12-01. These data include species. The instruments used to collect these data include Automated DNA Sequencer. These data were collected by Dr Stephen R. Palumbi of Stanford University and Luke Thomas of University of Western Australia as part of the "Ecological, evolutionary and physiological responses of corals to a mass bleaching event in American Samoa (Bleaching American Samoa)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-04-12.
The following is the text of the dataset description provided by BCO-DMO:
Dataset Description:
Coral colony genetic sequence accessions for samples collected from the lagoon environment of Ofu Island in the National Park of American Samoa between 2011 and 2015. All data and samples were collected at depths less than 3.0m.
This dataset includes all sequence data accession numbers for the publication Thomas & Palumbi (2017) housed at The National Center for Biotechnology Information under BioProject PRJNA522016 (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA522016).
The following is the text of the dataset description provided by BCO-DMO:
Dataset Description:
Coral colony genetic sequence accessions for samples collected from the lagoon environment of Ofu Island in the National Park of American Samoa between 2011 and 2015. All data and samples were collected at depths less than 3.0m.
This dataset includes all sequence data accession numbers for the publication Thomas & Palumbi (2017) housed at The National Center for Biotechnology Information under BioProject PRJNA522016 (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA522016).
Dataset Citation
- Cite as: Palumbi, Stephen R.; Thomas, Luke (2023). Coral colony genetic sequence accession numbers for samples collected from the lagoon environment of Ofu Island, American Samoa between 2011 and 2015 (NCEI Accession 0278347). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0278347. Accessed [date].
Dataset Identifiers
ISO 19115-2 Metadata
gov.noaa.nodc:0278347
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Ordering Instructions | Contact NCEI for other distribution options and instructions. |
Distributor |
NOAA National Centers for Environmental Information +1-301-713-3277 NCEI.Info@noaa.gov |
Dataset Point of Contact |
NOAA National Centers for Environmental Information ncei.info@noaa.gov |
Time Period | 2011-04-01 to 2015-12-01 |
Spatial Bounding Box Coordinates |
West: -169.655
East: -169.655
South: -14.18
North: -14.18
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Data Presentation Form | Digital table - digital representation of facts or figures systematically displayed, especially in columns |
Dataset Progress Status | Complete - production of the data has been completed Historical archive - data has been stored in an offline storage facility |
Data Update Frequency | As needed |
Supplemental Information | Acquisition Description: Seven colonies of A. hyacinthus from the back-reef environment of Ofu Island were tagged, sampled, and monitored at five time-points spanning the bleaching event: before bleaching representing baseline expression levels (August 2011), and then two months (April 2015), six months (August 2015), 10 months (December 2015) and 12 months (February 2016) after initial bleaching was observed in February 2015. Coral nubbins of approximately 24 cm in size were collected from colonies within 3 h of high-tide using garden clippers and preserved in RNAlater. All colonies occurred at depths of approximately 0.51.0 m and were within a 2500 m2area of back-reef. Total RNA was extracted from tissue samples using Qiagen's RNAeasy Plus Kit, and 35 cDNA libraries (seven colonies for five dates) were generated using the Illumina TruSeq RNA Library Prep Kit v2 with Protoscript II Reverse Transcriptase. The 35 libraries were multiplexed and sequenced across three lanes on a Hiseq2500 at the University of Utah Microarray and Genomic Analysis Core Facility. Location: Ofu Island, American Samoa. All data and samples were collected from the lagoon environment in the National Park of American Samoa at depths less than 3.0m. |
Purpose | This dataset is available to the public for a wide variety of uses including scientific research and analysis. |
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Data Center keywords | NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS Global Change Master Directory (GCMD) Data Center Keywords |
Platform keywords | BCO-DMO Platform Names |
Instrument keywords | BCO-DMO Standard Instruments Originator Instrument Names |
Place keywords | NODC SEA AREA NAMES THESAURUS Global Change Master Directory (GCMD) Location Keywords Provider Place Names |
Project keywords | BCO-DMO Standard Projects Provider Deployment IDs Provider Funding Award Information |
Keywords | NCEI ACCESSION NUMBER |
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Last Modified: 2024-05-31T15:15:28Z
For questions about the information on this page, please email: ncei.info@noaa.gov
For questions about the information on this page, please email: ncei.info@noaa.gov