Cell abundance estimates of heterotrophic protists by size-class, based on epifluorescence microscopy from samples collected on R/V Melville cruise MV1008 in the Costa Rica Dome in 2010 (CRD FLUZiE project) (NCEI Accession 0278314)
This dataset contains biological and survey - biological data collected on R/V Melville during cruise MV1008 in the North Pacific Ocean from 2010-07-04 to 2010-07-19. These data include abundance, depth, and taxon. The instruments used to collect these data include Camera and Inverted Microscope. These data were collected by Michael R. Landry of University of California-San Diego as part of the "Costa Rica Dome FLUx and Zinc Experiments (CRD FLUZiE)" project and "Integrated Marine Biogeochemistry and Ecosystem Research -US (IMBER-US)" and "Ocean Carbon and Biogeochemistry (OCB)" programs. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-12-30.
The following is the text of the dataset description provided by BCO-DMO:
Cell abundance estimates of heterotrophic protists by size-class, based on epifluorescence microscopy.
Dataset Description:
Cell abundance estimates of heterotrophic protists by size-class, based on epifluorescence microscopy. Samples were collected on the MV1008 cruise in the Costa Rica Dome (CRD) region of the Eastern Tropical Pacific Ocean.
The following is the text of the dataset description provided by BCO-DMO:
Cell abundance estimates of heterotrophic protists by size-class, based on epifluorescence microscopy.
Dataset Description:
Cell abundance estimates of heterotrophic protists by size-class, based on epifluorescence microscopy. Samples were collected on the MV1008 cruise in the Costa Rica Dome (CRD) region of the Eastern Tropical Pacific Ocean.
Dataset Citation
- Cite as: Landry, Michael R. (2023). Cell abundance estimates of heterotrophic protists by size-class, based on epifluorescence microscopy from samples collected on R/V Melville cruise MV1008 in the Costa Rica Dome in 2010 (CRD FLUZiE project) (NCEI Accession 0278314). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0278314. Accessed [date].
Dataset Identifiers
ISO 19115-2 Metadata
gov.noaa.nodc:0278314
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Ordering Instructions | Contact NCEI for other distribution options and instructions. |
Distributor |
NOAA National Centers for Environmental Information +1-301-713-3277 NCEI.Info@noaa.gov |
Dataset Point of Contact |
NOAA National Centers for Environmental Information ncei.info@noaa.gov |
Time Period | 2010-07-04 to 2010-07-19 |
Spatial Bounding Box Coordinates |
West: -92.987
East: -89.994
South: 8.392
North: 10.3
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Data Presentation Form | Digital table - digital representation of facts or figures systematically displayed, especially in columns |
Dataset Progress Status | Complete - production of the data has been completed Historical archive - data has been stored in an offline storage facility |
Data Update Frequency | As needed |
Supplemental Information | Acquisition Description: Seawater samples (500 mL) for microscopical analysis were gently collected from the CTD and immediately preserved for slide preparation according to a modified protocol of Sherr and Sherr (1993). The samples were first preserved with 260 uL of alkaline Lugol’s solution, immediately followed by 10 mL of buffered formalin and 500 uL of sodium thiosulfate, with gentle mixing between each addition. Preserved samples were shielded from light and left to rest at room temperature for 1 h. After the rest period, 1 mL of proflavin (0.33% w/v) was added and the samples were stored in the dark for an additional hour. Just prior to filtration, the preserved samples were stained with 1 mL of DAPI (0.01 mg mL -1 ) and immediately transferred to the filtration manifold. A 50-mL aliquot (small volume, SV) of the sample was filtered through a 25-mm black polycarbonate filter with 0.8-um pore size, and the remaining 450 mL aliquot (large volume, LV) was filtered through a 25-mm black polycarbonate filter with 8.0-um pores. A 10-mm nylon backing filter was placed under all polycarbonate filters to promote even cell distribution, and filtered the samples under gentle vacuum pressure (<100 mm Hg). Each filter was then mounted onto glass slides with one drop of Type DF immersion oil and a No. 2 cover slip, and the prepared slides were frozen at -80°C for later analysis in the lab. NOTE: ciliated protists are poorly preserved by this method and are not included in these analyses. |
Purpose | This dataset is available to the public for a wide variety of uses including scientific research and analysis. |
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Last Modified: 2024-06-26T15:35:16Z
For questions about the information on this page, please email: ncei.info@noaa.gov
For questions about the information on this page, please email: ncei.info@noaa.gov