Environmental and physical data associated with ocean acidification microbe adaptation from 2012-2014 (NCEI Accession 0278262)
This dataset contains biological, chemical, and physical data collected on Duke University Marine Lab during deployment PICO_1-301 from 2012-07-03 to 2014-12-31. These data include Ammonium, Nitrate, Nitrite, O2 saturation, SiOH_4, bacterial abundance, chlorophyll a, depth, dissolved Oxygen, dissolved inorganic Carbon, pH, reactive phosphorus (PO4), salinity calculated from CTD primary sensors, turbidity, water pressure, and water temperature. The instruments used to collect these data include Bottle, CO2 Analyzer, Dissolved Oxygen Sensor, Fluorometer, Niskin bottle, Nutrient Autoanalyzer, Pump, Refractometer, Spectrometer, Turbidity Meter, and digital thermometer. These data were collected by Dana Hunt and Dr Zackary I. Johnson of Duke University as part of the "Collaborative Research: Ocean Acidification: microbes as sentinels of adaptive responses to multiple stressors: contrasting estuarine and open ocean environments (OA microbe adaptation)" and "Pivers Island Coastal Observatory (PICO)" projects and "Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA) (SEES-OA)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-03-28.
The following is the text of the dataset description provided by BCO-DMO:
Environmental and physical data associated with ocean acidification microbe adaptation.
Dataset Description:
Environmental and physical data associated with ocean acidification microbe adaptation.
For related research, go to: http://oceanography.ml.duke.edu/johnson/research/pico/
The following is the text of the dataset description provided by BCO-DMO:
Environmental and physical data associated with ocean acidification microbe adaptation.
Dataset Description:
Environmental and physical data associated with ocean acidification microbe adaptation.
For related research, go to: http://oceanography.ml.duke.edu/johnson/research/pico/
Dataset Citation
- Cite as: Johnson, Zackary I.; Hunt, Dana (2023). Environmental and physical data associated with ocean acidification microbe adaptation from 2012-2014 (NCEI Accession 0278262). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0278262. Accessed [date].
Dataset Identifiers
ISO 19115-2 Metadata
gov.noaa.nodc:0278262
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Ordering Instructions | Contact NCEI for other distribution options and instructions. |
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NOAA National Centers for Environmental Information +1-301-713-3277 NCEI.Info@noaa.gov |
Dataset Point of Contact |
NOAA National Centers for Environmental Information ncei.info@noaa.gov |
Time Period | 2012-07-03 to 2014-12-31 |
Spatial Bounding Box Coordinates |
West: -76.6707
East: -76.6707
South: 34.7181
North: 34.7181
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Data Presentation Form | Digital table - digital representation of facts or figures systematically displayed, especially in columns |
Dataset Progress Status | Complete - production of the data has been completed Historical archive - data has been stored in an offline storage facility |
Data Update Frequency | As needed |
Supplemental Information | Acquisition Description: DIC: Water was sampled using a 5 L niskin bottle centered at 1 m with a bottle length of 0.7 m. DIC was measured on mercuric chloride poisoned samples by acidification and subsequent quantification of released CO2 using a CO2 detector (Li-Cor 7000). DIC samples were collected following recommended procedures {Dickson et al., 2007} and measurements were calibrated against Certified Reference Materials provided by Dr. A. G. Dickson at Scripps Institution of Oceanography (SIO), University of California, San Diego (UCSD). pH: Water was sampled using a 5 L niskin bottle centered at 1 m with a bottle length of 0.7 m. pH was measured spectrophotometrically {Clayton and Byrne, 1993} in triplicate at standard temperature (25°C) immediately following collection. pH samples were collected following recommended procedures {Dickson et al., 2007}. Secchi Depth: Secchi depth was measured in duplicate using a 20 cm disk with four alternating white and black quadrants (Cole Parmer #EW-05492-00) by lowering the disk until no longer visible and recording the depth. Salinity: Water was sampled using a 5 L niskin bottle centered at 1 m with a bottle length of 0.7 m. Salinity was measured using a calibrated handheld digital refractometer (Atago PAL-06S), using a refractometer (Vista A366ATC), or using a Guideline Portasal 8410A all according to manufacturer’s instructions and calibrated against known reference materials. In situ salinity at the same depth was measured using a YSI Pro30. Turbidity: Turbidity was measured in duplicate on discrete samples using a calibrated handheld turbidimeter (Orion AQ4500). Dissolved Oxygen: Oxygen was measured optically in situ and atmospheric pressure measured near the sea surface using a calibrated probe (YSI ProODO) using manufactures recommendations. Chlorophyll: Water was sampled using a 5 L niskin bottle centered at 1 m with a bottle length of 0.7 m. Methods described in Johnson et al. 2010: Chlorophyll concentrations were measured by filtering 25 mL of seawater sample onto a 0.22 µm pore size polycarbonate filter using gentle vacuum (<100 mm Hg) and extracting in 100% MeOH at -20°C in the dark for >24 h following (Holm-Hansen and Riemann, 1978). Fluorescence was measured using a Turner Designs 10-AU fluorometer following (Welschmeyer, 1994) that was calibrated against a standard chlorophyll solution (Ritchie, 2008). Bacteria: Bacterioplankton (i.e. ‘bacteria’) were enumerated using a FACSCalibur flow cytometer (Becton Dickinson) and populations characterized as previously described (Johnson et al., 2010). Briefly, cells were excited with a 488 nm laser (15 mW Ar) and inelastic forward (<15°) scatter, inelastic side (90°) scatter (SSC), green (530 ± 30 nm) fluorescence, orange fluorescence (585 ± 42 nm), and red fluorescence (> 670 nm) emissions were measured. Bacterioplankton were quantified by staining the samples with the nucleic acid stain SYBR Green –I (Molecular Probes Inc.) (Marie et al., 1997). Nutrients: Water was filtered through a 0.22 µm Sterivex cartridge filter, Millipore #SVGPL10RC using a peristaltic pump input line at 1 m for later nutrient analysis (NO3, NO2, PO4, SiOH4) and water was placed into duplicate HCl-cleaned HDPE bottles (VWR#414004-110) and stored at -80°C until later analysis using an Astoria-Pacific A2 autoanalyzer following the manufacturer’s recommended protocols running each replicate sample in duplicate. Certified reference materials were used to verify protocols (Inorganic Ventures: QCP-NT, QCP-NUT-1, CGSI1-1). The detection limit was NO2 = 0.05 µM, NO3 = 0.1 µM, PO4 = 0.05 µM, SiOH4 = 0.2 µM). Values measured below these limits are reported as zero. Temperature: Water was sampled using a 5 L niskin bottle centered at 1 m with a bottle length of 0.7 m. Temperature was measured in duplicate using NIST traceable thermocouples (VWR#23609-232). In situ water temperature at the same depth was measured using a YSI Pro30. |
Purpose | This dataset is available to the public for a wide variety of uses including scientific research and analysis. |
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Last Modified: 2024-05-31T15:15:28Z
For questions about the information on this page, please email: ncei.info@noaa.gov
For questions about the information on this page, please email: ncei.info@noaa.gov