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Picophytoplankton abundances determined by flow cytometry (post-cruise/on-shore analysis) from samples collected on R/V Melville cruise MV1008 in the Costa Rica Dome in 2010 (CRD FLUZiE project) (NCEI Accession 0278230)

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This dataset contains biological and survey - biological data collected on R/V Melville during cruise MV1008 in the North Pacific Ocean from 2010-06-24 to 2010-07-24. These data include depth, prochlorococcus abundance, and synechococcus abundance. The instruments used to collect these data include Flow Cytometer and Niskin bottle. These data were collected by Michael R. Landry of University of California-San Diego as part of the "Costa Rica Dome FLUx and Zinc Experiments (CRD FLUZiE)" project and "Integrated Marine Biogeochemistry and Ecosystem Research -US (IMBER-US)" and "Ocean Carbon and Biogeochemistry (OCB)" programs. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-12-27.

The following is the text of the dataset description provided by BCO-DMO:

Picophytoplankton abundances determined by flow cytometry (post-cruise/on-shore analysis).

Dataset Description:
Picophytoplankton abundances from preserved (0.5% paraformaldehyde) frozen samples run on a Beckman-Coulter Altra flow cytometer after staining with Hoechst 33342 DNA stain and excitation with 200 mW UV and 1W 488 nm laser light. Cell populations include Prochlorococcus , Synechococcus, and Picophytoeukaryotes. Samples were collected on the MV1008 cruise in the Costa Rica Dome (CRD) region of the Eastern Tropical Pacific Ocean.

Dataset Citation

Cite as: Landry, Michael R. (2023). Picophytoplankton abundances determined by flow cytometry (post-cruise/on-shore analysis) from samples collected on R/V Melville cruise MV1008 in the Costa Rica Dome in 2010 (CRD FLUZiE project) (NCEI Accession 0278230). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0278230. Accessed [date].

Dataset Identifiers

ISO 19115-2 Metadata

gov.noaa.nodc:0278230

Full Text · XML

Download Data	HTTPS (download) Navigate directly to the URL for data access and direct download. FTP (download) These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP).
Distribution Formats	TSV
Ordering Instructions	Contact NCEI for other distribution options and instructions.
Distributor	NOAA National Centers for Environmental Information +1-301-713-3277 NCEI.Info@noaa.gov
Dataset Point of Contact	NOAA National Centers for Environmental Information ncei.info@noaa.gov

Time Period	2010-06-24 to 2010-07-24
Spatial Bounding Box Coordinates	West: -92.987 East: -86.735 South: 6.622 North: 10.3
Spatial Coverage Map

General Documentation

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Descriptive Information
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Associated Resources

Biological, chemical, physical, biogeochemical, ecological, environmental and other data collected from around the world during historical and contemporary periods of biological and chemical oceanographic exploration and research managed and submitted by the Biological and Chemical Oceanography Data Management Office (BCO-DMO)
- NCEI Collection
  Navigate directly to the URL for data access and direct download.
Landry, M. R. (2014) Picophytoplankton abundances determined by flow cytometry (post-cruise/on-shore analysis) from samples collected on R/V Melville cruise MV1008 in the Costa Rica Dome in 2010 (CRD FLUZiE project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Dataset version 2014-06-02. https://doi.org/10.1575/1912/bco-dmo.516296.1
- https://doi.org/10.1575/1912/bco-dmo.516296.1 (download)
  originator dataset

gov.noaa.nodc:BCO-DMO

Publication Dates	publication: 2023-05-15
Data Presentation Form	Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status	Complete - production of the data has been completed Historical archive - data has been stored in an offline storage facility
Data Update Frequency	As needed
Supplemental Information	Acquisition Description: Samples were collected from the CTD-Rosette PVC Niskin bottles, then preserved with paraformaldehyde (0.5% final concentration). Preserved samples were then flash frozen in liquid nitrogen, and subsequently stored at -80°C until analysis on-shore in batches. Upon thawing, samples were stained with Hoechst 33342 DNA stain (1 ug/mL final concentration), incubated at room temperature in the dark for 1 h, then analyzed using a Beckman-Coulter Altra flow cytometer, equipped with one 488 nm 1W laser and one UV 200 mM laser for excitation. Samples (100 ul) were delivered quantitatively to the instrument using a Harvard Apparatus syringe pump, at a rate of 50 ul per minute. Signals from the DNA (450±40 nm), Chlorophyll (680±20 nm), Phycoerythrin (575±20 nm), and the forward and side scatter detectors were used to delineate Prochlorococcus , Synechococcus , and Picophytoeukaryotes. Calibration beads (0.5 um and 1.0 um yellow-green and 0.5 um UV) were used to normalize cellular fluorescence values and assure optimal instrument settings. Raw data files (listmode) were processed in FlowJo software (Treestar Inc.).
Purpose	This dataset is available to the public for a wide variety of uses including scientific research and analysis.
Use Limitations	accessLevel: Public Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.

Dataset Citation	Cite as: Landry, Michael R. (2023). Picophytoplankton abundances determined by flow cytometry (post-cruise/on-shore analysis) from samples collected on R/V Melville cruise MV1008 in the Costa Rica Dome in 2010 (CRD FLUZiE project) (NCEI Accession 0278230). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0278230. Accessed [date].
Cited Authors	Landry, Michael R. University of California-San Diego
Principal Investigators	Michael R. Landry University of California - San Diego; Scripps Institution of Oceanography (SIO)
Contributors	University of California - San Diego (UCSD)
Resource Providers	Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Points of Contact	Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Publishers	NOAA National Centers for Environmental Information
Acknowledgments	Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0826626 Award URL: http://www.nsf.gov/awardsearch/showAward?AWD_ID=0826626

Theme keywords	NODC DATA TYPES THESAURUS DEPTH - OBSERVATION Prochlorococcus species abundance Synechococcus NODC OBSERVATION TYPES THESAURUS biological survey - biological WMO_CategoryCode oceanography BCO-DMO Standard Parameters Niskin bottle number cast date depth event latitude longitude no standard parameter pico_euks prochlorococcus abundance synechococcus abundance Global Change Master Directory (GCMD) Science Keywords EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA > CYANOBACTERIA (BLUE-GREEN ALGAE) Originator Parameter Names cast coccus_p coccus_s cycle date_local depth event lat lon niskin peuk
Data Center keywords	NODC COLLECTING INSTITUTION NAMES THESAURUS University of California - San Diego NODC SUBMITTING INSTITUTION NAMES THESAURUS Biological and Chemical Oceanography Data Management Office Global Change Master Directory (GCMD) Data Center Keywords BCO-DMO > Biological and Chemical Oceanography Data Management Office
Platform keywords	NODC PLATFORM NAMES THESAURUS R/V Melville BCO-DMO Platform Names R/V Melville Global Change Master Directory (GCMD) Platform Keywords Ships ICES/SeaDataNet Ship Codes MELVILLE (call sign: WECB, ICES code: 318M, 1969)
Instrument keywords	NODC INSTRUMENT TYPES THESAURUS bottle Flow Cytometer BCO-DMO Standard Instruments Flow Cytometer Niskin bottle Global Change Master Directory (GCMD) Instrument Keywords FLOW CYTOMETRY Originator Instrument Names Beckman-Coulter Altra flow cytometer Niskin bottle
Place keywords	NODC SEA AREA NAMES THESAURUS North Pacific Ocean Global Change Master Directory (GCMD) Location Keywords OCEAN > PACIFIC OCEAN > NORTH PACIFIC OCEAN Provider Place Names Costa Rica Dome
Project keywords	BCO-DMO Standard Programs Integrated Marine Biogeochemistry and Ecosystem Research -US (IMBER-US) Ocean Carbon and Biogeochemistry (OCB) BCO-DMO Standard Projects Costa Rica Dome FLUx and Zinc Experiments (CRD FLUZiE) Provider Cruise IDs MV1008 Provider Funding Award Information Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0826626
Keywords	NCEI ACCESSION NUMBER 0278230

Use Constraints	Cite as: Landry, Michael R. (2023). Picophytoplankton abundances determined by flow cytometry (post-cruise/on-shore analysis) from samples collected on R/V Melville cruise MV1008 in the Costa Rica Dome in 2010 (CRD FLUZiE project) (NCEI Accession 0278230). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0278230. Accessed [date].
Data License	This dataset is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License. SPDX License: Creative Commons Attribution 4.0 International (CC-BY-4.0)
Access Constraints	Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
Fees	In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.

Lineage information for: dataset
Processing Steps	2023-05-15T04:06:38Z - NCEI Accession 0278230 v1.1 was published.
Output Datasets	NCEI Accession 0278230 v1.1 NCEI Accession 0278230 v1.1 (download) published 2023-05-15T04:06:38Z

Acquisition Information (collection)
Instrument	bottle Flow Cytometer
Platform	R/V Melville

Last Modified: 2024-06-26T19:24:12Z
For questions about the information on this page, please email: ncei.info@noaa.gov

Picophytoplankton abundances determined by flow cytometry (post-cruise/on-shore analysis) from samples collected on R/V Melville cruise MV1008 in the Costa Rica Dome in 2010 (CRD FLUZiE project) (NCEI Accession 0278230)

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