Single amplified genomes (SAGs) of microbial cells isolated from deep-sea hydrothermal vent 'Crab Spa', East Pacific Rise, Pacific Ocean from R/V Atlantis AT15-38 and AT26-10, 2008 and 2014 (Microbial Communities at Deep-Sea Vents project) (NCEI Accession 0278145)
This dataset contains biological data collected on R/V Atlantis during cruises AT15-38 and AT26-10 in the North Pacific Ocean from 2008-10-01 to 2014-01-15. These data include taxon and taxon_code. The instruments used to collect these data include Automated DNA Sequencer, Isobaric Gas-Tight Sampler, and ROV Jason. These data were collected by Dr Ramunas Stepanauskas of Bigelow Laboratory for Ocean Sciences as part of the "An Integrated Study of Energy Metabolism, Carbon Fixation, and Colonization Mechanisms in Chemosynthetic Microbial Communities at Deep-Sea Vents (Microbial Communities at Deep-Sea Vents)" project and "Center for Dark Energy Biosphere Investigations (C-DEBI)" and "Dimensions of Biodiversity (Dimensions of Biodiversity)" programs. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2021-04-07.
The following is the text of the dataset description provided by BCO-DMO:
Single amplified genomes (SAGs) of microbial cells isolated from Crab Spa, East Pacific Rise
Dataset Description:
The accession links are not yet public. They will be archived at the Integrated Microbial Genomes (IMG), at the Joint Genome Inst. (JGI), US Dept. of Energy (DOE). Please contact the PI for further details.
The following is the text of the dataset description provided by BCO-DMO:
Single amplified genomes (SAGs) of microbial cells isolated from Crab Spa, East Pacific Rise
Dataset Description:
The accession links are not yet public. They will be archived at the Integrated Microbial Genomes (IMG), at the Joint Genome Inst. (JGI), US Dept. of Energy (DOE). Please contact the PI for further details.
Dataset Citation
- Cite as: Stepanauskas, Ramunas (2023). Single amplified genomes (SAGs) of microbial cells isolated from deep-sea hydrothermal vent 'Crab Spa', East Pacific Rise, Pacific Ocean from R/V Atlantis AT15-38 and AT26-10, 2008 and 2014 (Microbial Communities at Deep-Sea Vents project) (NCEI Accession 0278145). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0278145. Accessed [date].
Dataset Identifiers
ISO 19115-2 Metadata
gov.noaa.nodc:0278145
Download Data |
|
Distribution Formats |
|
Ordering Instructions | Contact NCEI for other distribution options and instructions. |
Distributor |
NOAA National Centers for Environmental Information +1-301-713-3277 NCEI.Info@noaa.gov |
Dataset Point of Contact |
NOAA National Centers for Environmental Information ncei.info@noaa.gov |
Time Period | 2008-10-01 to 2014-01-15 |
Spatial Bounding Box Coordinates |
West: -104.291
East: -104.291
South: 9.84
North: 9.84
|
Spatial Coverage Map |
General Documentation |
|
Associated Resources |
|
Publication Dates |
|
Data Presentation Form | Digital table - digital representation of facts or figures systematically displayed, especially in columns |
Dataset Progress Status | Complete - production of the data has been completed Historical archive - data has been stored in an offline storage facility |
Data Update Frequency | As needed |
Supplemental Information | Acquisition Description: AT15-38: Samples for SAGs were obtained using the so-called 'titanium major samplers' (von Damm et al, 1985). Replicate samples of 1 ml aliquots of water were cryopreserved with 6% glycine betaine (Sigma) or 15% glycerol and stored at -80 ºC for the ”Single Cell” aliquot. AT26-10: Background samples were obtained from the IGTs, in which the incubations were carried out. Just in this case, an aliquot was removed after sample retrieval and before starting the incubation. During AT26-10, samples were preserved with Gly-TE and stored at -80. Cells were sorted, identified and sequenced by the Bigelow Laboratory Single Cell Genomics FacilityCenter (SCGC), following SCGCthe facilities’s standard practices: SCGC_Services_Description.pdf |
Purpose | This dataset is available to the public for a wide variety of uses including scientific research and analysis. |
Use Limitations |
|
Dataset Citation |
|
Cited Authors | |
Principal Investigators | |
Contributors | |
Resource Providers | |
Points of Contact | |
Publishers | |
Acknowledgments |
Theme keywords |
NODC DATA TYPES THESAURUS
NODC OBSERVATION TYPES THESAURUS
WMO_CategoryCode
|
Data Center keywords | NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS Global Change Master Directory (GCMD) Data Center Keywords |
Platform keywords | NODC PLATFORM NAMES THESAURUS BCO-DMO Platform Names Global Change Master Directory (GCMD) Platform Keywords ICES/SeaDataNet Ship Codes |
Instrument keywords | BCO-DMO Standard Instruments Originator Instrument Names |
Place keywords | NODC SEA AREA NAMES THESAURUS Global Change Master Directory (GCMD) Location Keywords Provider Place Names |
Project keywords |
BCO-DMO Standard Programs
|
Keywords | NCEI ACCESSION NUMBER |
Use Constraints |
|
Data License | |
Access Constraints |
|
Fees |
|
Lineage information for: dataset | |
---|---|
Processing Steps |
|
Output Datasets |
|
Acquisition Information (collection) | |
---|---|
Platform |
|
Last Modified: 2024-05-31T15:15:28Z
For questions about the information on this page, please email: ncei.info@noaa.gov
For questions about the information on this page, please email: ncei.info@noaa.gov