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Estimated abundances of viruses and bacteria determined in samples collected in the Eastern Tropical North Pacific (ETNP) on R/V New Horizon cruise NH1315 during June 2013 (NCEI Accession 0278126)

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This dataset contains biological and survey - biological data collected on R/V New Horizon during cruise NH1315 in the North Pacific Ocean from 2013-06-19 to 2013-06-22. These data include abundance and depth. The instruments used to collect these data include Fluorescence Microscope and Niskin bottle. These data were collected by Jennifer R. Brum of University of Arizona as part of the "Ecology and biogeochemical impacts of viruses in marine oxygen minimum zones (OMZ Viruses)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2020-09-16.

The following is the text of the dataset description provided by BCO-DMO:

Estimated abundances of viruses and bacteria via epifluorescence microscopy in the ETNP

Dataset Description:
Estimated abundances of viruses and bacteria via epifluorescence microscopy of samples collected in the Eastern Tropical North Pacific oxygen minimum zone region (ETNP OMZ) on R/V New Horizon cruise NH1315 from 13-28 June 2013.
  • Cite as: Brum, Jennifer R. (2023). Estimated abundances of viruses and bacteria determined in samples collected in the Eastern Tropical North Pacific (ETNP) on R/V New Horizon cruise NH1315 during June 2013 (NCEI Accession 0278126). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0278126. Accessed [date].
gov.noaa.nodc:0278126
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Distributor NOAA National Centers for Environmental Information
+1-301-713-3277
NCEI.Info@noaa.gov
Dataset Point of Contact NOAA National Centers for Environmental Information
ncei.info@noaa.gov
Time Period 2013-06-19 to 2013-06-22
Spatial Bounding Box Coordinates
West: -108.799
East: -104.89
South: 18.92
North: 18.92
Spatial Coverage Map
General Documentation
Associated Resources
  • Biological, chemical, physical, biogeochemical, ecological, environmental and other data collected from around the world during historical and contemporary periods of biological and chemical oceanographic exploration and research managed and submitted by the Biological and Chemical Oceanography Data Management Office (BCO-DMO)
    • NCEI Collection
      Navigate directly to the URL for data access and direct download.
  • Brum, J. R. (2020) Estimated abundances of viruses and bacteria determined in samples collected in the Eastern Tropical North Pacific (ETNP) on R/V New Horizon cruise NH1315 during June 2013. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-09-02. https://doi.org/10.26008/1912/bco-dmo.823094.1
    • Parent ID (indicates this dataset is related to other data):
    • gov.noaa.nodc:BCO-DMO
Publication Dates
  • publication: 2023-05-13
Data Presentation Form Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status Complete - production of the data has been completed
Historical archive - data has been stored in an offline storage facility
Data Update Frequency As needed
Supplemental Information
Acquisition Description:
Detailed protocols, including suggestions from the scientific community, are published on the lab website at https://u.osu.edu/viruslab/protocols/ and maintained on protocols.io at https://www.protocols.io/workspaces/sullivan-lab .

Samples were collected from the Eastern Tropical North Pacific oxygen minimum zone region (ETNP OMZ) during the OMZ Microbial Biogeochemistry Expedition cruise (R/V NewHorizon,13-28 June 2013). Seawater was collected from 16 depths spanning the mixed layer, oxycline, OMZ core, and below the OMZ. Collections were made using Niskin bottles on a rosette. Samples were preserved with EM-grade glutaraldehyde (2% final concentration), flash-frozen in liquid nitrogen and stored between -72 °C and -80 °C until analysis.

Viral and bacterial concentrations were determined based on a previously described method (Noble and Fuhrman, 1998) in which thawed samples were filtered onto 0.02-μm- pore-size filters (Anodisc, Whatman, GE Healthcare Life Sciences, Piscataway, NJ, USA), stained with SYBR Gold nucleic acid stain (Invitrogen, Life Technologies, Carlsbad, CA, USA) and enumerated using an epifluorescence microscope (Axio Imager. D2, Zeiss, Jena, Germany).
Purpose This dataset is available to the public for a wide variety of uses including scientific research and analysis.
Use Limitations
  • accessLevel: Public
  • Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.
Dataset Citation
  • Cite as: Brum, Jennifer R. (2023). Estimated abundances of viruses and bacteria determined in samples collected in the Eastern Tropical North Pacific (ETNP) on R/V New Horizon cruise NH1315 during June 2013 (NCEI Accession 0278126). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0278126. Accessed [date].
Cited Authors
Principal Investigators
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Acknowledgments
Theme keywords NODC DATA TYPES THESAURUS NODC OBSERVATION TYPES THESAURUS WMO_CategoryCode
  • oceanography
BCO-DMO Standard Parameters Originator Parameter Names
Data Center keywords NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS Global Change Master Directory (GCMD) Data Center Keywords
Platform keywords NODC PLATFORM NAMES THESAURUS BCO-DMO Platform Names Global Change Master Directory (GCMD) Platform Keywords ICES/SeaDataNet Ship Codes
Instrument keywords NODC INSTRUMENT TYPES THESAURUS BCO-DMO Standard Instruments Global Change Master Directory (GCMD) Instrument Keywords Originator Instrument Names
Place keywords NODC SEA AREA NAMES THESAURUS Global Change Master Directory (GCMD) Location Keywords Provider Place Names
Project keywords BCO-DMO Standard Projects Provider Cruise IDs Provider Funding Award Information
Keywords NCEI ACCESSION NUMBER
Use Constraints
  • Cite as: Brum, Jennifer R. (2023). Estimated abundances of viruses and bacteria determined in samples collected in the Eastern Tropical North Pacific (ETNP) on R/V New Horizon cruise NH1315 during June 2013 (NCEI Accession 0278126). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0278126. Accessed [date].
Data License
Access Constraints
  • Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
Fees
  • In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.
Lineage information for: dataset
Processing Steps
  • 2023-05-13T04:14:30Z - NCEI Accession 0278126 v1.1 was published.
Output Datasets
Acquisition Information (collection)
Instrument
  • bottle
  • fluorescence microscope
Platform
  • NEW HORIZON
Last Modified: 2024-05-31T15:15:28Z
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