Prochlorococcus in situ cell cycle phases fractions from RV Cape Hatteras cruises CH0409 and CH0510 in the Western Sargasso Sea in 2009 and 2010 (NCEI Accession 0277937)
This dataset contains biological and optical data collected on R/V Cape Hatteras during cruises CH0409 and CH0510 from 2009-05-26 to 2010-05-31. These data include depth and fluorescence. The instruments used to collect these data include Flow Cytometer and Niskin bottle. These data were collected by Dr Brian Binder of University of Georgia as part of the "Top-Down Regulation of Picophytoplankton in the Sargasso Sea: Application of a Reciprocal Transplant / Dilution Approach (Picophytoplankton_Regulation)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-03-19.
The following is the text of the dataset description provided by BCO-DMO:
Prochlorococcus in situ Cell Cycle Phases Fractions.
Dataset Description:
Prochlorococcus in situ Cell Cycle Phases Fractions.
The following is the text of the dataset description provided by BCO-DMO:
Prochlorococcus in situ Cell Cycle Phases Fractions.
Dataset Description:
Prochlorococcus in situ Cell Cycle Phases Fractions.
Dataset Citation
- Cite as: Binder, Brian (2023). Prochlorococcus in situ cell cycle phases fractions from RV Cape Hatteras cruises CH0409 and CH0510 in the Western Sargasso Sea in 2009 and 2010 (NCEI Accession 0277937). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0277937. Accessed [date].
Dataset Identifiers
ISO 19115-2 Metadata
gov.noaa.nodc:0277937
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Ordering Instructions | Contact NCEI for other distribution options and instructions. |
Distributor |
NOAA National Centers for Environmental Information +1-301-713-3277 NCEI.Info@noaa.gov |
Dataset Point of Contact |
NOAA National Centers for Environmental Information ncei.info@noaa.gov |
Time Period | 2009-05-26 to 2010-05-31 |
Spatial Bounding Box Coordinates |
West: -72.8769
East: -71.8634
South: 30.1464
North: 30.9082
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Data Presentation Form | Digital table - digital representation of facts or figures systematically displayed, especially in columns |
Dataset Progress Status | Complete - production of the data has been completed Historical archive - data has been stored in an offline storage facility |
Data Update Frequency | As needed |
Supplemental Information | Acquisition Description: Samples were taken using a rosette of Niskin bottles, fixed with freshly titrated paraformaldehyde (pH 7.4–8.1, 0.1% final concentration), held in the dark for 10 min, frozen in liquid nitrogen, and stored in a -80 deg C freezer (CH0409 samples) or in liquid nitrogen (CH0510 samples) until analysis. Preserved samples were analyzed by dual beam flow cytometry on a modified Coulter-EPICS 753 flow cytometer (Binder et al. 1996). Samples were chosen in random order, defrosted in a 30°C water bath (just long enough to melt, ~5 min), and stained with the DNA-specific stain Hoechst 33342 (0.5 ug mL-1 final concentration) (Invitrogen, Carlsbad, California) for a minimum of 20 min in the dark. Prior to analysis, polystyrene fluorescent beads (Flow Check® 1.0 um (YG) and 0.494 um (BB); Polysicences Inc., Washington, PA, USA), were added to each sample, and used to normalize cellular light scatter, red (chlorophyll-derived) fluorescence, and Hoechst fluorescence. Samples were run at an infusion rate of 10 uL min-1 for 10 to 50 min, depending on cell abundance within the sample. A minimum of 10,000 Prochlorococcus cells were analyzed, except for samples in which low Prochlorococcus concentrations made this impractical. DNA frequency distributions for Prochlorococcus cells were obtained from Hoechst-derived blue fluorescence. These frequency distributions were deconvoluted into their component cell cycle stages (G1, S, G2) using Modfit software (Verity Software House, Topsham, ME, USA), and assuming a simple model comprised of two Gaussian populations (G1 and G2) and a broadened rectangle (S). |
Purpose | This dataset is available to the public for a wide variety of uses including scientific research and analysis. |
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NODC DATA TYPES THESAURUS
NODC OBSERVATION TYPES THESAURUS
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Data Center keywords | NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS Global Change Master Directory (GCMD) Data Center Keywords |
Platform keywords | NODC PLATFORM NAMES THESAURUS BCO-DMO Platform Names Global Change Master Directory (GCMD) Platform Keywords ICES/SeaDataNet Ship Codes |
Instrument keywords | NODC INSTRUMENT TYPES THESAURUS BCO-DMO Standard Instruments Global Change Master Directory (GCMD) Instrument Keywords Originator Instrument Names |
Place keywords | Provider Place Names |
Project keywords | BCO-DMO Standard Projects Provider Cruise IDs Provider Funding Award Information |
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Last Modified: 2024-05-31T15:15:28Z
For questions about the information on this page, please email: ncei.info@noaa.gov
For questions about the information on this page, please email: ncei.info@noaa.gov