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Counts of Prochlorococcus from on-deck incubations with 13C-bicarbonate as part of DNA-SIP experiments conducted on Hawaii Ocean Time-series (HOT) cruises, HOT283 and HOT288 in 2016 (NCEI Accession 0277931)

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This dataset contains biological and survey - biological data collected on R/V Ka`imikai-O-Kanaloa and R/V Sikuliaq during cruises KOK1605 and SKQ201615S at Station Aloha (22 45N, 158W), Hawaii Ocean Time series (HOT) from 2016-04-13 to 2016-11-29. These data include prochlorococcus abundance. The instruments used to collect these data include CTD profiler, Flow Cytometer, and Trace Metal Bottle. These data were collected by Anne Thompson of Portland State University as part of the "Microbial ecology of coexisting ecotypes: Are all Prochlorococcus equal? (ProEco)" project and "Ocean Time-series Sites (Ocean Time-series)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-08-02.

The following is the text of the dataset description provided by BCO-DMO:

Counts of Prochlorococcus from on-deck incubations with 13C-bicarbonate as part of DNA-SIP experiments

Dataset Description:
Counts of Prochlorococcus from on-deck incubations with 13C-bicarbonate as part of DNA-SIP experiments; conducted on Hawaii Ocean Time-series (HOT) cruises 283 and 288.

Dataset Citation

Cite as: Thompson, Anne (2023). Counts of Prochlorococcus from on-deck incubations with 13C-bicarbonate as part of DNA-SIP experiments conducted on Hawaii Ocean Time-series (HOT) cruises, HOT283 and HOT288 in 2016 (NCEI Accession 0277931). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0277931. Accessed [date].

Dataset Identifiers

ISO 19115-2 Metadata

gov.noaa.nodc:0277931

Full Text · XML

Download Data	HTTPS (download) Navigate directly to the URL for data access and direct download. FTP (download) These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP).
Distribution Formats	TSV
Ordering Instructions	Contact NCEI for other distribution options and instructions.
Distributor	NOAA National Centers for Environmental Information +1-301-713-3277 NCEI.Info@noaa.gov
Dataset Point of Contact	NOAA National Centers for Environmental Information ncei.info@noaa.gov

Coverage Description	Station Aloha (22 45N, 158W), Hawaii Ocean Time series (HOT)
Time Period	2016-04-13 to 2016-11-29
Spatial Bounding Box Coordinates	West: East: South: North:
Spatial Coverage Map

General Documentation

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Descriptive Information
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Associated Resources

Biological, chemical, physical, biogeochemical, ecological, environmental and other data collected from around the world during historical and contemporary periods of biological and chemical oceanographic exploration and research managed and submitted by the Biological and Chemical Oceanography Data Management Office (BCO-DMO)
- https://www.ncei.noaa.gov/archive/accession/BCO-DMO
  NCEI Collection
Thompson, A. (2017) Counts of Prochlorococcus from on-deck incubations with 13C-bicarbonate as part of DNA-SIP experiments conducted on Hawaii Ocean Time-series (HOT) cruises, HOT283 and HOT288 in 2016. Biological and Chemical Oceanography Data Management Office (BCO-DMO). Dataset version 2017-05-23. https://doi.org/10.1575/1912/bco-dmo.700773.1
- https://doi.org/10.1575/1912/bco-dmo.700773.1 (download)
  originator dataset

gov.noaa.nodc:BCO-DMO

Publication Dates	publication: 2023-05-06
Data Presentation Form	Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status	Complete - production of the data has been completed Historical archive - data has been stored in an offline storage facility
Data Update Frequency	As needed
Supplemental Information	Acquisition Description: Water was sampled from the CTD under trace metal clean conditions into 2 L polycarbonate bottles (acid-washed) and amended with 13C-bicarbonate. Flow cytometry samples were collected from triplicate bottles at several time points between 0 hours (unlabeled control) and 36 hours after the initiation of incubation. Flow cytometry samples were collected in 1 mL volumes and fixed immediately with 25% TEM-grade glutaraldehyde to a final concentrations of 0.125%. Samples were inverted 10 times to mix, incubated at room temperature in the dark for 10 minutes, then flash frozen in liquid nitrogen to archive. Samples were stored at -80C until analysis. For analysis, each sample was thawed at room temperature then analyzed by flow cytometry. Cell counts were determined by flow cytometry using a BD Biosciences Influx high speed cell sorter. A 488 nm laser was used in addition to chlorophyll and phycoerythrin filters, forward scatter relative to 1 um beads, and side scattered light.
Purpose	This dataset is available to the public for a wide variety of uses including scientific research and analysis.
Use Limitations	accessLevel: Public Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.

Dataset Citation	Cite as: Thompson, Anne (2023). Counts of Prochlorococcus from on-deck incubations with 13C-bicarbonate as part of DNA-SIP experiments conducted on Hawaii Ocean Time-series (HOT) cruises, HOT283 and HOT288 in 2016 (NCEI Accession 0277931). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0277931. Accessed [date].
Cited Authors	Thompson, Anne Portland State University
Principal Investigators	Anne Thompson Portland State University (PSU)
Contributors	Portland State University (PSU)
Resource Providers	Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Points of Contact	Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Publishers	NOAA National Centers for Environmental Information
Acknowledgments	Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1646709 Award URL: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1646709

Theme keywords	NODC DATA TYPES THESAURUS Prochlorococcus species abundance NODC OBSERVATION TYPES THESAURUS biological survey - biological WMO_CategoryCode oceanography BCO-DMO Standard Parameters cruise id depth_comment experiment id prochlorococcus abundance replicate sample time Global Change Master Directory (GCMD) Science Keywords EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA > CYANOBACTERIA (BLUE-GREEN ALGAE) Originator Parameter Names cruise depth incubation prochlorococcus replicate timepoint
Data Center keywords	NODC COLLECTING INSTITUTION NAMES THESAURUS Portland State University NODC SUBMITTING INSTITUTION NAMES THESAURUS Biological and Chemical Oceanography Data Management Office Global Change Master Directory (GCMD) Data Center Keywords BCO-DMO > Biological and Chemical Oceanography Data Management Office
Platform keywords	NODC PLATFORM NAMES THESAURUS KA'IMIKAI-O-KANALOA Sikuliaq BCO-DMO Platform Names R/V Ka`imikai-O-Kanaloa R/V Sikuliaq Global Change Master Directory (GCMD) Platform Keywords Ships ICES/SeaDataNet Ship Codes KA'IMIKAI-O-KANALOA (call sign: WBN4310, ICES code: 33KI, 1995) Sikuliaq (call sign: WDG7520, ICES code: 33BI, 2013)
Instrument keywords	NODC INSTRUMENT TYPES THESAURUS CTD Flow Cytometer BCO-DMO Standard Instruments CTD profiler Flow Cytometer Trace Metal Bottle Global Change Master Directory (GCMD) Instrument Keywords CTD > Conductivity, Temperature, Depth FLOW CYTOMETRY Originator Instrument Names BD Biosciences Influx high speed cell sorter CTD unknown (Trace Metal Bottle)
Place keywords	Provider Place Names Station Aloha (22 45N, 158W), Hawaii Ocean Time series (HOT)
Project keywords	BCO-DMO Standard Programs Ocean Time-series Sites (Ocean Time-series) BCO-DMO Standard Projects Microbial ecology of coexisting ecotypes: Are all Prochlorococcus equal? (ProEco) Provider Cruise IDs KOK1605 SKQ201615S Provider Funding Award Information Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1646709
Keywords	NCEI ACCESSION NUMBER 0277931

Use Constraints	Cite as: Thompson, Anne (2023). Counts of Prochlorococcus from on-deck incubations with 13C-bicarbonate as part of DNA-SIP experiments conducted on Hawaii Ocean Time-series (HOT) cruises, HOT283 and HOT288 in 2016 (NCEI Accession 0277931). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0277931. Accessed [date].
Data License	This dataset is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License. SPDX License: Creative Commons Attribution 4.0 International (CC-BY-4.0)
Access Constraints	Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
Fees	In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.

Lineage information for: dataset
Processing Steps	2023-05-06T04:07:27Z - NCEI Accession 0277931 v1.1 was published.
Output Datasets	NCEI Accession 0277931 v1.1 NCEI Accession 0277931 v1.1 (download) published 2023-05-06T04:07:27Z

Acquisition Information (collection)
Instrument	CTD Flow Cytometer
Platform	KA'IMIKAI-O-KANALOA Sikuliaq

Last Modified: 2024-09-16T21:36:20Z
For questions about the information on this page, please email: ncei.info@noaa.gov

Counts of Prochlorococcus from on-deck incubations with 13C-bicarbonate as part of DNA-SIP experiments conducted on Hawaii Ocean Time-series (HOT) cruises, HOT283 and HOT288 in 2016 (NCEI Accession 0277931)

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