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Dataset Overview | National Centers for Environmental Information (NCEI)

Single colony metaproteomes of Trichodesmium from samples collected in North Atlantic surface waters during the R/V Atlantis cruise AT39-05 in March of 2018 (NCEI Accession 0277719)

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This dataset contains biological and survey - biological data collected on R/V Atlantis during cruise AT39-05 on 2018-03-11. These data include abundance and taxon. The instruments used to collect these data include Mass Spectrometer. These data were collected by Mak A. Saito of Woods Hole Oceanographic Institution as part of the "Collaborative Research: Iron and phosphorus balanced limitation of nitrogen fixation in the oligotrophic ocean (TriCoLim)", "Collaborative Research: Evolutionary, biochemical and biogeochemical responses of marine cyanobacteria to warming and iron limitation interactions (Cyanobacteria Warming Responses)", "Marine Microbial Investigator Award: Investigator Mak Saito (MM Saito)", and "New technology for high resolution analysis of proteins and other organic materials produced by marine microorganisms (MM Proteins and Organics Tech)" projects and "Marine Microbiology Initiative (MMI)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2020-10-08.

The following is the text of the dataset description provided by BCO-DMO:

Dataset Description:
Single colony metaproteomes of Trichodesmium from samples collected in North Atlantic surface waters during the R/V Atlantis cruise AT39-05 in March of 2018. These data were published in Held et al. (2020).

The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE [1] partner repository with the dataset identifier PXD016330 and 10.6019/PXD016330 but are not yet public.
* Project Name: Trichodesmium single colony metaproteomes field station Tricolim_19
* Project accession: PXD016330
* Project https://doi.org/10.6019/PXD016330
  • Cite as: Saito, Mak A. (2023). Single colony metaproteomes of Trichodesmium from samples collected in North Atlantic surface waters during the R/V Atlantis cruise AT39-05 in March of 2018 (NCEI Accession 0277719). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0277719. Accessed [date].
gov.noaa.nodc:0277719
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Distributor NOAA National Centers for Environmental Information
+1-301-713-3277
NCEI.Info@noaa.gov
Dataset Point of Contact NOAA National Centers for Environmental Information
ncei.info@noaa.gov
Time Period 2018-03-11 to 2018-03-11
Spatial Bounding Box Coordinates
West: 65.22
East: 65.22
South: 17.02
North: 17.02
Spatial Coverage Map
General Documentation
Associated Resources
  • Biological, chemical, physical, biogeochemical, ecological, environmental and other data collected from around the world during historical and contemporary periods of biological and chemical oceanographic exploration and research managed and submitted by the Biological and Chemical Oceanography Data Management Office (BCO-DMO)
    • NCEI Collection
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  • Saito, M. A. (2020) Single colony metaproteomes of Trichodesmium from samples collected in North Atlantic surface waters during the R/V Atlantis cruise AT39-05 in March of 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-01-10. https://doi.org/10.26008/1912/bco-dmo.786694.1
  • Parent ID (indicates this dataset is related to other data):
    • gov.noaa.nodc:BCO-DMO
Publication Dates
  • publication: 2023-04-20
Data Presentation Form Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status Complete - production of the data has been completed
Historical archive - data has been stored in an offline storage facility
Data Update Frequency As needed
Supplemental Information
Acquisition Description:
A 200mm plankton net was deployed to ~20m depth, then recovered to just below surface, repeating five times. Trichodesmium colonies were hand-picked into 0.2mm filtered surface seawater, rinsed twice in 0.2mm filtered surface seawater, and decanted onto a 0.2-4mm Supor filter (indicated in sample provenance table, see dataset https://www.bco-dmo.org/dataset/787093).

Proteins were extracted and trypsin digested in-gel following Saito et al., 2014 (Science) with the following modifications: organic precipitation was skipped, benzonase nuclease treatment was performed instead to dissolve DNA/RNA material. Peptides were analyzed by LC-MS/MS in data discovery mode on a Thermo Orbitrap Fusion. Full details will be reported in Held et al. (in prep)

Location: North Atlantic surface waters 65.22 W 17.02 N
Purpose This dataset is available to the public for a wide variety of uses including scientific research and analysis.
Use Limitations
  • accessLevel: Public
  • Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.
Dataset Citation
  • Cite as: Saito, Mak A. (2023). Single colony metaproteomes of Trichodesmium from samples collected in North Atlantic surface waters during the R/V Atlantis cruise AT39-05 in March of 2018 (NCEI Accession 0277719). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0277719. Accessed [date].
Cited Authors
Principal Investigators
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Points of Contact
Publishers
Acknowledgments
Theme keywords NODC DATA TYPES THESAURUS NODC OBSERVATION TYPES THESAURUS WMO_CategoryCode
  • oceanography
BCO-DMO Standard Parameters Originator Parameter Names
Data Center keywords NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS Global Change Master Directory (GCMD) Data Center Keywords
Platform keywords NODC PLATFORM NAMES THESAURUS BCO-DMO Platform Names Global Change Master Directory (GCMD) Platform Keywords ICES/SeaDataNet Ship Codes
Instrument keywords NODC INSTRUMENT TYPES THESAURUS BCO-DMO Standard Instruments Global Change Master Directory (GCMD) Instrument Keywords Originator Instrument Names
Place keywords Provider Place Names
Project keywords BCO-DMO Standard Programs BCO-DMO Standard Projects Provider Cruise IDs Provider Funding Award Information
Keywords NCEI ACCESSION NUMBER
Use Constraints
  • Cite as: Saito, Mak A. (2023). Single colony metaproteomes of Trichodesmium from samples collected in North Atlantic surface waters during the R/V Atlantis cruise AT39-05 in March of 2018 (NCEI Accession 0277719). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0277719. Accessed [date].
Data License
Access Constraints
  • Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
Fees
  • In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.
Lineage information for: dataset
Processing Steps
  • 2023-04-20T04:45:34Z - NCEI Accession 0277719 v1.1 was published.
Output Datasets
Acquisition Information (collection)
Instrument
  • mass spectrometer
Platform
  • R/V Atlantis
Last Modified: 2024-06-04T18:34:55Z
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