Data from fish genotyped at 14 and 20 loci at different life stages in the Belizean Barrier Reef in 2013 (NCEI Accession 0277399)
This dataset contains biological and survey - biological data collected at lab Buston during deployment Belize_2010 from 2013-06-01 to 2013-06-30. These data include stage. The instruments used to collect these data include GPS receiver. These data were collected by Dr Peter Buston of Boston University and Dr Cassidy C. D'Aloia of Woods Hole Oceanographic Institution as part of the "An Integrative Investigation of Population Connectivity Using a Coral Reef Fish (Elacatinus Dispersal I)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2023-01-23.
The following is the text of the dataset description provided by BCO-DMO:
Data from fish genotyped at 14 and 20 loci at different life stages in the Belizean Barrier Reef in 2013.
Dataset Description:
Data from fish genotyped at 14 and 20 loci at different life stages. Sampling completed on the Belizean Barrier Reef in 2013.
The following is the text of the dataset description provided by BCO-DMO:
Data from fish genotyped at 14 and 20 loci at different life stages in the Belizean Barrier Reef in 2013.
Dataset Description:
Data from fish genotyped at 14 and 20 loci at different life stages. Sampling completed on the Belizean Barrier Reef in 2013.
Dataset Citation
- Cite as: Buston, Peter; D'Aloia, Cassidy C. (2023). Data from fish genotyped at 14 and 20 loci at different life stages in the Belizean Barrier Reef in 2013 (NCEI Accession 0277399). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0277399. Accessed [date].
Dataset Identifiers
ISO 19115-2 Metadata
gov.noaa.nodc:0277399
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Ordering Instructions | Contact NCEI for other distribution options and instructions. |
Distributor |
NOAA National Centers for Environmental Information +1-301-713-3277 NCEI.Info@noaa.gov |
Dataset Point of Contact |
NOAA National Centers for Environmental Information ncei.info@noaa.gov |
Time Period | 2013-06-01 to 2013-06-30 |
Spatial Bounding Box Coordinates |
West: -88.075833
East: -88.075833
South: 16.789722
North: 16.789722
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Data Presentation Form | Digital table - digital representation of facts or figures systematically displayed, especially in columns |
Dataset Progress Status | Complete - production of the data has been completed Historical archive - data has been stored in an offline storage facility |
Data Update Frequency | As needed |
Supplemental Information | Acquisition Description: We surveyed a 41 km-long transect of the Belize Barrier reef, centered at Carrie Bow Cay, by SCUBA to conduct a genetic parentage study of the reef fish Elactinus lori . All underwater sampling was conducted using SCUBA at an average (±SD) depth of 16.03 ± 2.19 m. A waypoint was recorded from the boat at the beginning and end of every collection dive, with the midpoint of each dive taken as the location for all individuals sampled on that dive. To collect settlers, we sampled ~ 100 individuals every kilometer. Individuals were collected from the outsides of sponges using slurp guns and placed them in plastic bags. At the surface, settlers were anesthetized with MS-222. For adults, we collected non-lethal tissue samples at three regions along the transect (n ≈ 1,000 per region). Each adult was collected with a slurp gun and restrained in a net; we took a small tissue sample from the caudal fin using scissors. All tissue was stored in 95% EtOH. At each adult collection sponge, we also measured: sponge depth (m, using dive computers), number of tubes per sponge, and length of largest sponge tube (nearest cm, using a tape measure). For genetic analyses, DNA was extracted using a HotSHOT protocol; fragments were amplified using the Type-It Microsatellite PCR Kit (Qiagen) and screened on an ABI 3730 automated sequencer. Otoliths were extracted from the 120 settlers that were assigned to parents. Otoliths were dissected, cleared of tissue, immersed in oil for 2-7 days, and rings were counted under a 50× oil immersion lens Further details on all methods can be found in D’Aloia et al. (2015), PNAS. |
Purpose | This dataset is available to the public for a wide variety of uses including scientific research and analysis. |
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Data Center keywords | NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS Global Change Master Directory (GCMD) Data Center Keywords |
Platform keywords | BCO-DMO Platform Names Global Change Master Directory (GCMD) Platform Keywords |
Instrument keywords | NODC INSTRUMENT TYPES THESAURUS BCO-DMO Standard Instruments Global Change Master Directory (GCMD) Instrument Keywords Originator Instrument Names |
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Project keywords | BCO-DMO Standard Projects Provider Deployment IDs Provider Funding Award Information |
Keywords | NCEI ACCESSION NUMBER |
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Last Modified: 2024-09-16T21:36:20Z
For questions about the information on this page, please email: ncei.info@noaa.gov
For questions about the information on this page, please email: ncei.info@noaa.gov