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Dataset Overview | National Centers for Environmental Information (NCEI)

Flow cytometry results for naupliar grazing laboratory experiments conducted from 2012-2013 (EAGER: Copepod nauplii project) (NCEI Accession 0277298)

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This dataset contains biological and survey - biological data collected at lab UHawaii_SOEST during deployment Goetze_2012-2013 from 2013-05-29 to 2013-06-05. These data include abundance. The instruments used to collect these data include Flow Cytometer. These data were collected by Dr Petra Lenz, Erica Goetze, and Karen E. Selph of University of Hawaii at Manoa as part of the "New molecular methods for studying copepod nauplii in the field (EAGER: Copepod nauplii)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2023-01-23.

The following is the text of the dataset description provided by BCO-DMO:

Flow cytometry results for naupliar grazing experiments (laboratory)

Dataset Description:
These data report initial and final measurements of prey abundances in bottle incubation experiments to measure naupliar grazing.
  • Cite as: Goetze, Erica; Lenz, Petra; Selph, Karen E. (2023). Flow cytometry results for naupliar grazing laboratory experiments conducted from 2012-2013 (EAGER: Copepod nauplii project) (NCEI Accession 0277298). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0277298. Accessed [date].
gov.noaa.nodc:0277298
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Distributor NOAA National Centers for Environmental Information
+1-301-713-3277
NCEI.Info@noaa.gov
Dataset Point of Contact NOAA National Centers for Environmental Information
ncei.info@noaa.gov
Time Period 2013-05-29 to 2013-06-05
Spatial Bounding Box Coordinates
West: -157.7797
East: -157.7797
South: 21.4322
North: 21.4322
Spatial Coverage Map
General Documentation
Associated Resources
  • Biological, chemical, physical, biogeochemical, ecological, environmental and other data collected from around the world during historical and contemporary periods of biological and chemical oceanographic exploration and research managed and submitted by the Biological and Chemical Oceanography Data Management Office (BCO-DMO)
    • NCEI Collection
      Navigate directly to the URL for data access and direct download.
  • Copepod nauplii project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2016-02-04. https://doi.org/10.26008/1912/bco-dmo.637720.1
    • Parent ID (indicates this dataset is related to other data):
    • gov.noaa.nodc:BCO-DMO
Publication Dates
  • publication: 2023-03-30
Data Presentation Form Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status Complete - production of the data has been completed
Historical archive - data has been stored in an offline storage facility
Data Update Frequency As needed
Supplemental Information
Acquisition Description:
All samples were collected from surface water in Southern Kaneohe Bay, Oahu, Hawaii (21°25’56.7”N, 157°46’47.1”W).

Pro, Syn, Peuk, PE+Peuk, HBact data: These data were collected and analyzed by flow cytometry as described in Selph et al. (2011). Briefly, samples were preserved (0.05% paraformaldehyde, final concentration), and frozen to -80°C within 1 h. Batches of samples were thawed, stained with Hoechst 34442 (1 µg/mL final concentration) and analyzed on a flow cytometer (Beckman Coulter Altra) with dual laser excitation (UV range and 488 nm, 200 mW and 1 W, respectively), mated to a Harvard Apparatus syringe pump for quantitative delivery (100 µl at 50 µl min -1 ). Resulting listmode files were analyzed off-line using FlowJo software (Treestar). Populations determined, based on red fluorescence (chlorophyll, 680±20 nm), orange fluorescence (phycoerythrin, 575±20 nm), blue fluorescence (DNA, 450±40 nm), and light scatter signals (forward and 90°). Distinguishable populations were Prochlorococcus , Synechococcus , Photosynthetic Eukaryotes (PEUK), High phycoerythrin-containing PEUK (PE+Peuk), and non-pigmented prokaryotes (a.k.a. heterotrophic bacteria, HBACT).
Purpose This dataset is available to the public for a wide variety of uses including scientific research and analysis.
Use Limitations
  • accessLevel: Public
  • Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.
Dataset Citation
  • Cite as: Goetze, Erica; Lenz, Petra; Selph, Karen E. (2023). Flow cytometry results for naupliar grazing laboratory experiments conducted from 2012-2013 (EAGER: Copepod nauplii project) (NCEI Accession 0277298). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0277298. Accessed [date].
Cited Authors
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Acknowledgments
Theme keywords NODC DATA TYPES THESAURUS NODC OBSERVATION TYPES THESAURUS WMO_CategoryCode
  • oceanography
BCO-DMO Standard Parameters Originator Parameter Names
Data Center keywords NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS Global Change Master Directory (GCMD) Data Center Keywords
Platform keywords BCO-DMO Platform Names Global Change Master Directory (GCMD) Platform Keywords
Instrument keywords NODC INSTRUMENT TYPES THESAURUS BCO-DMO Standard Instruments Global Change Master Directory (GCMD) Instrument Keywords Originator Instrument Names
Place keywords Provider Place Names
Project keywords BCO-DMO Standard Projects Provider Deployment IDs Provider Funding Award Information
Keywords NCEI ACCESSION NUMBER
Use Constraints
  • Cite as: Goetze, Erica; Lenz, Petra; Selph, Karen E. (2023). Flow cytometry results for naupliar grazing laboratory experiments conducted from 2012-2013 (EAGER: Copepod nauplii project) (NCEI Accession 0277298). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0277298. Accessed [date].
Data License
Access Constraints
  • Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
Fees
  • In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.
Lineage information for: dataset
Processing Steps
  • 2023-03-30T22:42:26Z - NCEI Accession 0277298 v1.1 was published.
Output Datasets
Acquisition Information (collection)
Instrument
  • Flow Cytometer
Last Modified: 2024-05-31T18:50:46Z
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