Chlorophyll measurements from the MesoHux mesocosm experiment held in May 2017 (NCEI Accession 0277283)
This dataset contains biological and optical data collected during deployment MesoHux_2017 at Raunefjord, 20 miles south of Bergen from 2017-05-12 to 2017-05-30. These data include chl_tot, chlorophyll a, depth, fluorescence, and total phaeopigment. The instruments used to collect these data include Turner Designs Fluorometer 10-AU. These data were collected by Kay D. Bidle of Rutgers University, Elizabeth Harvey of University of Georgia, and Matthew D. Johnson of Woods Hole Oceanographic Institution as part of the "Quantifying competing loss rates of viral lysis and microzooplankton grazing on Emiliania huxleyi mortality (E huxleyi Mortality)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2023-01-23.
The following is the text of the dataset description provided by BCO-DMO:
Dataset Description:
Water samples for chlorophyll extraction were collected from the mesocosms via a 5 L Niskin. Chlorophyll samples were filtered in triplicate through a 25mm Glass Fiber Filter (GFF), and immediately extracted in 6 mL of ethanol for 12-18 hours in the dark at room temperature. After extraction, filters were removed from the sample, and the fluorescence of the sample was read on a Turner AU10. The sample was then acidified with 1 drop of 10% HCL and re-read on the same instrument. The fluorometer was calibrated prior to using with a chlorophyll standard purchased from Sigma.
Mesocosm treatments are as follows:
P-limited: N:P added in a 60:1 ratio during the first 3 days of the experiment, no shading
Redfield: N:P added in a 16:1 ratio during the first 3 days of the experiment, no shading
Shaded: N:P added in a 16:1 ratio during the first 3 days of the experiment, top shaded of the mesocosm added on May 20, 2017
Ambient: no nutrients added, no shading
Samples were taken from either 1m, 5m, or from sediment from settling cones. Samples were noted in the Sample Depth (m) column.
The following is the text of the dataset description provided by BCO-DMO:
Dataset Description:
Water samples for chlorophyll extraction were collected from the mesocosms via a 5 L Niskin. Chlorophyll samples were filtered in triplicate through a 25mm Glass Fiber Filter (GFF), and immediately extracted in 6 mL of ethanol for 12-18 hours in the dark at room temperature. After extraction, filters were removed from the sample, and the fluorescence of the sample was read on a Turner AU10. The sample was then acidified with 1 drop of 10% HCL and re-read on the same instrument. The fluorometer was calibrated prior to using with a chlorophyll standard purchased from Sigma.
Mesocosm treatments are as follows:
P-limited: N:P added in a 60:1 ratio during the first 3 days of the experiment, no shading
Redfield: N:P added in a 16:1 ratio during the first 3 days of the experiment, no shading
Shaded: N:P added in a 16:1 ratio during the first 3 days of the experiment, top shaded of the mesocosm added on May 20, 2017
Ambient: no nutrients added, no shading
Samples were taken from either 1m, 5m, or from sediment from settling cones. Samples were noted in the Sample Depth (m) column.
Dataset Citation
- Cite as: Johnson, Matthew D.; Bidle, Kay D.; Harvey, Elizabeth (2023). Chlorophyll measurements from the MesoHux mesocosm experiment held in May 2017 (NCEI Accession 0277283). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0277283. Accessed [date].
Dataset Identifiers
ISO 19115-2 Metadata
gov.noaa.nodc:0277283
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| Coverage Description | Raunefjord, 20 miles south of Bergen |
| Time Period | 2017-05-12 to 2017-05-30 |
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Last Modified: 2024-05-31T15:15:28Z
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For questions about the information on this page, please email: ncei.info@noaa.gov
