Viral and bacterial counts from filtered water, stained with SYBR Green and counted using epifluorescent microscopy, from samples collected on R/V Knorr cruise KN210-04 in the Western Atlantic Ocean in 2013 (Deep Atlantic DOM project) (NCEI Accession 0277273)
This dataset contains biological, physical, and survey - biological data collected on R/V Knorr during cruise KN210-04 in the North Atlantic Ocean and South Atlantic Ocean from 2013-03-26 to 2013-05-06. These data include abundance, bacterial abundance, depth, and water pressure. The instruments used to collect these data include Fluorescence Microscope. These data were collected by Ian Hewson of Cornell University as part of the "Dissolved Organic Matter Composition in the Deep Atlantic Ocean (Deep Atlantic DOM)" project and "Ocean Carbon and Biogeochemistry (OCB)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2023-01-23.
The following is the text of the dataset description provided by BCO-DMO:
Viral and bacterial counts from filtered water, stained with SYBR Green and counted using epifluorescent microscopy.
Dataset Description:
Viral and bacterial counts from filtered water, stained with SYBR Green and counted using epifluorescent microscopy. Duplicates were collected at each depth during the KN210-04 cruise.
The following is the text of the dataset description provided by BCO-DMO:
Viral and bacterial counts from filtered water, stained with SYBR Green and counted using epifluorescent microscopy.
Dataset Description:
Viral and bacterial counts from filtered water, stained with SYBR Green and counted using epifluorescent microscopy. Duplicates were collected at each depth during the KN210-04 cruise.
Dataset Citation
- Cite as: Hewson, Ian (2023). Viral and bacterial counts from filtered water, stained with SYBR Green and counted using epifluorescent microscopy, from samples collected on R/V Knorr cruise KN210-04 in the Western Atlantic Ocean in 2013 (Deep Atlantic DOM project) (NCEI Accession 0277273). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0277273. Accessed [date].
Dataset Identifiers
ISO 19115-2 Metadata
gov.noaa.nodc:0277273
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Ordering Instructions | Contact NCEI for other distribution options and instructions. |
Distributor |
NOAA National Centers for Environmental Information +1-301-713-3277 NCEI.Info@noaa.gov |
Dataset Point of Contact |
NOAA National Centers for Environmental Information ncei.info@noaa.gov |
Time Period | 2013-03-26 to 2013-05-06 |
Spatial Bounding Box Coordinates |
West: -55.304
East: -23.999
South: -38.003
North: 9.704
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Data Presentation Form | Digital table - digital representation of facts or figures systematically displayed, especially in columns |
Dataset Progress Status | Complete - production of the data has been completed Historical archive - data has been stored in an offline storage facility |
Data Update Frequency | As needed |
Supplemental Information | Acquisition Description: Samples for viral and bacterial counts were collected in 50 mL Falcon tubes, held at 4 degrees C until processing, and processed within two hours. SYBR slides were prepared according to Noble and Fuhruman (Noble and Fuhrman, 1998). Briefly, samples were fixed in v/v 2% formalin and then filtered over a 25 mm 0.02 um anodisc filter (Whatman). Varying volumes were fixed depending on the depth of sample collection (surface and DCM: 3 ml; mesopelagic: 10 ml; AAIW, NADW and AABW: 20 ml). The filter was stained with 2.5x10-3 SYBR Green 1 dilution in nuclease free water, and mounted on a slide with antifade solution (0.1% p-phenylenediamine in 1:1 PBS:Glycerol) and stored at -20 degrees C until enumeration. Under epifluorescence microscopy 10 fields of view were selected at random and >200 viruses and >200 bacteria were counted. Calculations for total number or viruses and bacteria in a sample account for volume filtered, magnification and size of the grid included while counting. Samples from station 1 and 2 were fixed with the wrong concentration of antifade and therefore faded before accurate counts could be made. References: Noble, R. T., and Fuhrman, J. A. (1998). Use of SYBR Green I for rapid epifluorescence counts of marine viruses and bacteria. Aquat Microb Ecol 14 , 113-118. doi: 10.3354/ame014113 |
Purpose | This dataset is available to the public for a wide variety of uses including scientific research and analysis. |
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Place keywords | NODC SEA AREA NAMES THESAURUS Global Change Master Directory (GCMD) Location Keywords Provider Place Names |
Project keywords | BCO-DMO Standard Programs BCO-DMO Standard Projects Provider Cruise IDs Provider Funding Award Information |
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Last Modified: 2024-05-31T15:15:28Z
For questions about the information on this page, please email: ncei.info@noaa.gov
For questions about the information on this page, please email: ncei.info@noaa.gov