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Laboratory experimental growth and development responses of larval walleye pollock (Gadus chalcogrammus) experiencing ocean acidification conditions from 2018-04-04 to 2018-05-17 (NCEI Accession 0258039)

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This dataset contains data from manipulated experimental seawater chemistry conditions and walleye pollock (Gadus chalcogrammus) larval development, swimming behavior, and lipid composition responses. The experiment took place from April 4-May 17, 2018 in the Alaska Fisheries Science Center laboratory research facilities at Hatfield Marine Science Center in Newport, Oregon. Larvae were obtained from natural spawning of laboratory-acclimated broodstock adults. Experiments occurred from fertilization to 4 weeks post-hatch at ambient (~ 425 µatm) and elevated (~ 1230 µatm) CO2 levels. This effort was conducted in support of the research objectives of the NOAA Ocean Acidification Program (OAP).
  • Cite as: Hurst, Thomas P.; Copeman, Louise A.; Andrade, Jessica F.; Stowell, Michelle A.; Al-Samarrie, Colleen E.; Sanders, Justin L.; Kent, Michael L. (2022). Laboratory experimental growth and development responses of larval walleye pollock (Gadus chalcogrammus) experiencing ocean acidification conditions from 2018-04-04 to 2018-05-17 (NCEI Accession 0258039). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0258039. Accessed [date].
gov.noaa.nodc:0258039
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Distributor NOAA National Centers for Environmental Information
+1-301-713-3277
NCEI.Info@noaa.gov
Dataset Point of Contact NOAA National Centers for Environmental Information
ncei.info@noaa.gov
Time Period 2018-04-04 to 2018-05-17
Spatial Bounding Box Coordinates
West: -122.76
East: -122.76
South: 48.136
North: 48.136
Spatial Coverage Map
General Documentation
Associated Resources
  • Hurst TP, Copeman LA, Andrade JF, Stowell MA, Al-Samarrie CE, Sanders JL, Kent ML (2021). Expanding evaluation of ocean acidification responses in a marine gadid: elevated CO2 impacts development, but not size of larval walleye pollock. Mar Biol 168:119. https://doi.org/10.1007/s00227-021-03924-w
  • NOAA National Centers for Environmental Information (2022). Ocean Carbon and Acidification Data System (OCADS). NOAA National Centers for Environmental Information. https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system
Publication Dates
  • publication: 2022-08-11
Data Presentation Form Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status Complete - production of the data has been completed
Historical archive - data has been stored in an offline storage facility
Data Update Frequency As needed
Supplemental Information
This data package (Submission ID: BF1537UYC) was acquired by NCEI from the Scientific Data Integration System (SDIS) at the NOAA Pacific Marine Environmental Laboratory (PMEL) in accordance with the archival submission agreement between NCEI and PMEL.

Scientific Abstract:
Responses of marine populations to climate conditions reflect the integration of a suite of complex and interrelated physiological and behavioral responses at the individual level. Many of these responses are not immediately reflected in changes to survival, but may impact growth or survival at later life stages. Understanding the broad range of impacts of rising CO2 concentrations on marine fishes is critical to predicting the consequences of ongoing ocean acidification. Walleye pollock (Gadus chalcogrammus) support the largest single-species fishery in the world and provide a critical forage base throughout north Pacific ecosystems. Previous studies of high CO2 effects on early life stages of walleye pollock have suggested a general resiliency in this species, but those studies focused primarily on growth and survival rates. Here, we expand on earlier studies with an independent experiment focused on walleye pollock larval development, swimming behavior, and lipid composition from fertilization to 4 weeks post-hatch at ambient (~ 425 µatm) and elevated (~ 1230 µatm) CO2 levels. Consistent with previous observations, size metrics of walleye pollock were generally insensitive to CO2 treatment. However, 4-week post-hatch larvae had significantly reduced rates of swim bladder inflation. A modest change in the swimming behavior of post-feeding larvae was observed at four, but not at 2 weeks post-hatch. Although there were no differences in overall lipid levels between CO2 treatments, the ratio of energy storage lipids (triacylglycerols) to structural membrane lipids (sterols) was lower among larvae reared at high CO2 levels. Although we observed higher survival to 4 weeks post-hatch among fish reared at high CO2 levels, the observations of reduced swim bladder inflation rates and changes in lipid cycling suggest the presence of sub-lethal effects of acidification that may carry over and manifest in later life stages. These observations support the continued need to evaluate the impacts of ocean acidification on marine fishes across a wide range of traits and life stages with replicated, independent experiments.
Purpose To determine the effects of CO2 on behavior, growth, and health of walleye pollock larvae
Use Limitations
  • accessLevel: Public
  • Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.
Dataset Citation
  • Cite as: Hurst, Thomas P.; Copeman, Louise A.; Andrade, Jessica F.; Stowell, Michelle A.; Al-Samarrie, Colleen E.; Sanders, Justin L.; Kent, Michael L. (2022). Laboratory experimental growth and development responses of larval walleye pollock (Gadus chalcogrammus) experiencing ocean acidification conditions from 2018-04-04 to 2018-05-17 (NCEI Accession 0258039). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0258039. Accessed [date].
Cited Authors
Principal Investigators
Contributors
Resource Providers
Publishers
Acknowledgments
  • Funding Information: NOAA Ocean Acidification Program (Mapping OA impacts: Modeling the Multiple Action Pathways of OA effects on Alaska gadids, OAP-AFSC-FY20-001)
  • Funding Information: NOAA's Ocean Acidification Program (Effects of OA on Alaskan groundfishes: identifying patterns and mechanisms of sensitivity and resiliency in physiological and behavioral performance, T8R3CEB-P00)
Theme keywords NODC DATA TYPES THESAURUS NODC OBSERVATION TYPES THESAURUS WMO_CategoryCode
  • oceanography
Global Change Master Directory (GCMD) Science Keywords OCADS Study Type
  • Laboratory experiment
Provider Variable Abbreviations
  • 16.1_ n.7
  • 18.1_n.7
  • 18.1_n.9
  • 18.2_n.6
  • 20.4_n.6
  • 20.5_n.3
  • 22.5_n.3
  • 22.5_n.6
  • 22.6_n.3
  • CO2_trt
  • DIC
  • DWT_mean
  • DWT_sd
  • FFA_percent
  • H.T_ID
  • LabSpawn_expt
  • MUFA_percent
  • PL_percent
  • PUFA_percent
  • SB
  • SB_size
  • SFA_percent
  • ST_percent
  • TA
  • TAG_ST
  • TAG_percent
  • Wild_expt
  • _14.0
  • _16.0
  • _18.0
  • bottle_ID
  • calcAr
  • calcCO2
  • calcpH
  • count_1
  • count_2
  • count_3
  • date
  • dph
  • fish_SL
  • h2oDQ
  • k_DWT_mean
  • k_DWT_sd
  • lab
  • larv_rep
  • life_stage
  • lipid_ID
  • mean_BD
  • mean_ED
  • mean_SL_1
  • mean_SL_2
  • mean_win.k_BD
  • salinity
  • sd_BD
  • sd_ED
  • sd_SL_1
  • sd_SL_2
  • sd_win.k_BD
  • strikes
  • swims
  • tank
  • temp
  • time
  • time_fed
  • total.lipid_DWT
  • total.lipid_indiv
  • turns
  • week
Data Center keywords NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS Global Change Master Directory (GCMD) Data Center Keywords
Instrument keywords NODC INSTRUMENT TYPES THESAURUS Global Change Master Directory (GCMD) Instrument Keywords
Place keywords Provider Geographic Names
  • NOAA AFSC Laboratory
  • Newport
  • Oregon
Project keywords NODC PROJECT NAMES THESAURUS Ocean Acidification Search Keywords
  • Ocean Acidification Program (OAP)
  • Ocean Carbon and Acidification Data System (OCADS) Project
Keywords NCEI ACCESSION NUMBER
Use Constraints
  • Cite as: Hurst, Thomas P.; Copeman, Louise A.; Andrade, Jessica F.; Stowell, Michelle A.; Al-Samarrie, Colleen E.; Sanders, Justin L.; Kent, Michael L. (2022). Laboratory experimental growth and development responses of larval walleye pollock (Gadus chalcogrammus) experiencing ocean acidification conditions from 2018-04-04 to 2018-05-17 (NCEI Accession 0258039). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0258039. Accessed [date].
Access Constraints
  • Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
Fees
  • In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.
Lineage information for: dataset
Processing Steps
  • 2022-08-11T20:57:34Z - NCEI Accession 0258039 v1.1 was published.
Output Datasets
Lineage information for: dataset
Processing Steps
  • Parameter or Variable: Dissolved Inorganic Carbon; Abbreviation: DIC; Unit: μmol/kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: Measured; Sampling instrument: Siphoning tube; Analyzing instrument: AIRICA (Automated InfraRed Inorganic Carbon Analyzer); Preservation method: saturated HgCl2; Preservative correction: none; Uncertainty: <0.05%; Quality flag convention: "h2oDQ" reflects reliability of data: 1=good; 2=questionable/outlier/estimated; 3=known error; Researcher name: Natalie Monacci; Researcher institution: Ocean Acidification Research Center at the University of Alaska Fairbanks.
  • Parameter or Variable: Total alkalinity; Abbreviation: TA; Unit: μmol/kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: Measured; Sampling instrument: Siphoning tube; Analyzing instrument: VINDTA 3C (Versatile Instrument for the Determination of dissolved inorganic carbon and Total Alkalinity); CRM manufacturer: Andrew Dickson's lab at Scripps Institute of Oceanography; Preservation method: saturated HgCl2; Preservative correction: none; Uncertainty: <0.5%; Quality flag convention: "h2oDQ" reflects reliability of data: 1=good; 2=questionable/outlier/estimated; 3=known error; Researcher name: Natalie Monacci; Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks.
  • Parameter or Variable: pH; Abbreviation: calcpH; pH scale: seawater; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: Calculated; Sampling instrument: Siphoning tube; Detailed sampling and analyzing information: calculated from DIC, TA, temperature, and salinity; At what temperature was pH reported: 5.9±0.4°C; Quality flag convention: "h2oDQ" reflects reliability of data: 1=good; 2=questionable/outlier/estimated; 3=known error; Method reference: Lewis and Wallace 1995. Basic programs for the CO2 system in seawater. Brookhaven National Laboratory. BNL-61827; Researcher name: Natalie Monacci; Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks.
  • Parameter or Variable: pCO2 (fCO2) autonomous; Abbreviation: calcCO2; Unit: µatm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: Calculated; Sampling instrument: Siphoning tube; Detailed sampling and analyzing information: calculated from DIC, TA, temperature, and salinity; Quality flag convention: "h2oDQ" reflects reliability of data: 1=good; 2=questionable/outlier/estimated; 3=known error; Method reference: Lewis and Wallace 1995. Basic programs for the CO2 system in seawater. Brookhaven National Laboratory. BNL-61827; Researcher name: Natalie Monacci; Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks.
  • Parameter or Variable: water temperature; Abbreviation: temp; Unit: °C; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: Measured; Analyzing instrument: various; Researcher name: Thomas P. Hurst; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: water salinity; Abbreviation: salinity; Unit: ppt; Observation type: Laboratory experiment; Analyzing instrument: Yellow Springs International meter - Model YSI 85; Researcher name: Thomas P. Hurst; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: aragonite saturation state; Abbreviation: calcAr; Unit: omega; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: Calculated; Detailed sampling and analyzing information: calculated using CO2sys program; Quality flag convention: "h2oDQ" reflects reliabiltity of data: 1=good; 2=questionable/outlier/estimated; 3=known error; Researcher name: Natalie Monacci; Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks.
  • Parameter or Variable: data quality flag for CO2 system measures; Abbreviation: h2oDQ; Unit: coded: 1=good; 2=questionable/outlier/estimated; 3=known error; Observation type: Laboratory experiment; Researcher name: Thomas P. Hurst; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: number of swimming movements by an individual; Abbreviation: swims; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Calculated; Detailed sampling and analyzing information: count of distinct forward swims within a one-minute observation period occurring thirty minutes after introducing food; Replicate information: up to 6 fish were observed from each treatment replicate on each sampling date; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: number of turns made by an individual; Abbreviation: turns; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Calculated; Detailed sampling and analyzing information: count of distinct turns within a one-minute observation period occurring thirty minutes after introducing food; Replicate information: up to 6 fish were observed from each treatment replicate on each sampling date; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: number of food strikes made by an individual; Abbreviation: strikes; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Calculated; Detailed sampling and analyzing information: count of food strikes within a one-minute observation period occurring thirty minutes after introducing food; Replicate information: up to 6 fish were observed from each treatment replicate on each sampling date; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: swim bladder presence/absence; Abbreviation: SB; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Calculation method and parameters: coded as: 1 = swim bladder present, or 0 = swim bladder absent; Sampling instrument: dissecting microscope; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 20 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 15 fish were measured from each treatment replicate on each sampling date; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Michael L. Kent; Researcher institution: Department of Microbiology, Oregon State University, Corvallis, OR, USA.
  • Parameter or Variable: swim bladder size; Abbreviation: SB_size; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: maximum length of the swim bladder; Sampling instrument: dissecting microscope; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 20 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 15 fish were measured from each treatment replicate on each sampling date; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Michael L. Kent; Researcher institution: Department of Microbiology, Oregon State University, Corvallis, OR, USA.
  • Parameter or Variable: fish standard length; Abbreviation: fish_SL; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: dissecting microscope; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 20 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 15 fish were measured from each treatment replicate on each sampling date; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Michael L. Kent; Researcher institution: Department of Microbiology, Oregon State University, Corvallis, OR, USA.
  • Parameter or Variable: mean standard length 1; Abbreviation: mean_SL_1; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: dissecting microscope; Analyzing instrument: ImagePro image analysis system; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 15 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 15 fish were measured from each treatment replicate on each sampling date; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: standard deviation of mean standard length 1; Abbreviation: sd_SL_1; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: dissecting microscope; Analyzing instrument: ImagePro image analysis system; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 15 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 15 fish were measured from each treatment replicate on each sampling date; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: mean body depth; Abbreviation: mean_BD; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: dissecting microscope; Analyzing instrument: ImagePro image analysis system; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 15 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 15 fish were measured from each treatment replicate on each sampling date; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: standard deviation of mean body depth; Abbreviation: sd_BD; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: dissecting microscope; Analyzing instrument: ImagePro image analysis system; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 15 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 15 fish were measured from each treatment replicate on each sampling date; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: mean eye diameter; Abbreviation: mean_ED; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: dissecting microscope; Analyzing instrument: ImagePro image analysis system; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 15 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 15 fish were measured from each treatment replicate on each sampling date; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: standard deviation of mean eye diameter; Abbreviation: sd_ED; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: dissecting microscope; Analyzing instrument: ImagePro image analysis system; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 15 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 15 fish were measured from each treatment replicate on each sampling date; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: mean body condition - expressed as winsorized fractional deviation from the ontogenetic relationship between SL & BD; Abbreviation: mean_win.k_BD; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 15 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 15 fish were measured from each treatment replicate on each sampling date; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: standard deviation of mean body condition - expressed as winsorized fractional deviation from the ontogenetic relationship between SL & BD; Abbreviation: sd_win.k_BD; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 15 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 15 fish were measured from each treatment replicate on each sampling date; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: mean standard length 2; Abbreviation: mean_SL_2; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: dissecting microscope; Analyzing instrument: ImagePro image analysis system; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 15 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 15 fish were measured from each treatment replicate on each sampling date; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: standard deviation of mean standard length 2; Abbreviation: sd_SL_2; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: dissecting microscope; Analyzing instrument: ImagePro image analysis system; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 15 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 15 fish were measured from each treatment replicate on each sampling date; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: mean dry weight; Abbreviation: DWT_mean; Unit: μg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Analyzing instrument: Sartorius Microbalance ME5; Detailed sampling and analyzing information: Fish were rinsed in ammonium formate and dried at 50°C for at least 48 hours prior to measurement; Replicate information: Fish were weighed in pooled samples of up to 5 larvae and up to 3 times per tank and sampling event; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: standard deviation of mean dry weight; Abbreviation: DWT_sd; Unit: μg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Analyzing instrument: Sartorius Microbalance ME5; Detailed sampling and analyzing information: Fish were rinsed in ammonium formate and dried at 50°C for at least 48 hours prior to measurement; Replicate information: Fish were weighed in pooled samples of up to 5 larvae and up to 3 times per tank and sampling event; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: mean body condition - expressed as fractional deviation from the ontogenetic relationship between SL & DWT; Abbreviation: k_DWT_mean; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Calculated; Detailed sampling and analyzing information: Fish were rinsed in ammonium formate and dried at 50°C for at least 48 hours prior to measurement; Replicate information: Fish were weighed in pooled samples of up to 5 larvae and up to 3 times per tank and sampling event; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: standard deviation of mean body condition - expressed as fractional deviation from the ontogenetic relationship between SL & DWT; Abbreviation: k_DWT_sd; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Detailed sampling and analyzing information: Fish were rinsed in ammonium formate and dried at 50°C for at least 48 hours prior to measurement; Replicate information: Fish were weighed in pooled samples of up to 5 larvae and up to 3 times per tank and sampling event; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: percentage of total lipids composed of triacylglycerols in the lipid sample; Abbreviation: TAG_percent; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Calculation method and parameters: chromatogram peak area was used with a calibration curve based on a triacylglycerol standard purified from gadid liver; Analyzing instrument: Iatroscan TLC-FID (MK-6s, Iatron Laboratories) with PeakSimple integration software (ver. 3.67, SRI Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. Lipid classes were determined using thin layer chromatography with flame ionization detection, by micropipetting lipid extracts onto duplicate silica-coated glass rods, developing the rods in a three-stage solvent system to separate lipid classes, scanning the rods on a MK-6s Iatroscan TLC-FID, and integrating the resulting chromatograms using integration software with a calibration curve.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Lu et al. (2008) Can J Fish Aquat Sci 65:2233-2241; Copeman et al. (2017) Mar Ecol Prog Ser 566:183-198.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: percentage of total lipids composed of free fatty acids in the lipid sample; Abbreviation: FFA_percent; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Calculation method and parameters: chromatogram peak area was used with a calibration curve based on a commercial (Sigma) palmitic acid standard; Analyzing instrument: Iatroscan TLC-FID (MK-6s, Iatron Laboratories) with PeakSimple integration software (ver. 3.67, SRI Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. Lipid classes were determined using thin layer chromatography with flame ionization detection, by micropipetting lipid extracts onto duplicate silica-coated glass rods, developing the rods in a three-stage solvent system to separate lipid classes, scanning the rods on a MK-6s Iatroscan TLC-FID, and integrating the resulting chromatograms using integration software with a calibration curve.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Lu et al. (2008) Can J Fish Aquat Sci 65:2233-2241; Copeman et al. (2017) Mar Ecol Prog Ser 566:183-198.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: percentage of total lipids composed of sterols; Abbreviation: ST_percent; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: chromatogram peak area was used with a calibration curve based on a commercial (Sigma) cholesterol standard; Analyzing instrument: Iatroscan TLC-FID (MK-6s, Iatron Laboratories) with PeakSimple integration software (ver. 3.67, SRI Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. Lipid classes were determined using thin layer chromatography with flame ionization detection, by micropipetting lipid extracts onto duplicate silica-coated glass rods, developing the rods in a three-stage solvent system to separate lipid classes, scanning the rods on a MK-6s Iatroscan TLC-FID, and integrating the resulting chromatograms using integration software with a calibration curve.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Lu et al. (2008) Can J Fish Aquat Sci 65:2233-2241; Copeman et al. (2017) Mar Ecol Prog Ser 566:183-198.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: percentage of total lipids composed of polar lipids in the lipid sample; Abbreviation: PL_percent; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: chromatogram peak area was used with a calibration curve based on a commercial (Sigma) L-alpha-phosphatidylcholine standard; Analyzing instrument: Iatroscan TLC-FID (MK-6s, Iatron Laboratories) with PeakSimple integration software (ver. 3.67, SRI Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. Lipid classes were determined using thin layer chromatography with flame ionization detection, by micropipetting lipid extracts onto duplicate silica-coated glass rods, developing the rods in a three-stage solvent system to separate lipid classes, scanning the rods on a MK-6s Iatroscan TLC-FID, and integrating the resulting chromatograms using integration software with a calibration curve.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Lu et al. (2008) Can J Fish Aquat Sci 65:2233-2241; Copeman et al. (2017) Mar Ecol Prog Ser 566:183-198.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: ratio of triacylglycerol to sterol in lipid sample; Abbreviation: TAG_ST; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: chromatogram peak area was used with a calibration curve based on a commercial (Sigma) L-alpha-phosphatidylcholine standard; Analyzing instrument: Iatroscan TLC-FID (MK-6s, Iatron Laboratories) with PeakSimple integration software (ver. 3.67, SRI Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. Lipid classes were determined using thin layer chromatography with flame ionization detection, by micropipetting lipid extracts onto duplicate silica-coated glass rods, developing the rods in a three-stage solvent system to separate lipid classes, scanning the rods on a MK-6s Iatroscan TLC-FID, and integrating the resulting chromatograms using integration software with a calibration curve.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Lu et al. (2008) Can J Fish Aquat Sci 65:2233-2241; Copeman et al. (2017) Mar Ecol Prog Ser 566:183-198.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: total lipids per dry weight of lipid sample; Abbreviation: total.lipid_DWT; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: Sum of all lipid class amounts in the lipid sample (TAG, FFA, ST, PL, in ug) divided by an estimate of the lipid sample dry weight (mg). Lipid sample dry weight estimated by multiplying mean dry weight per larva by number of larvae in lipid sample.; Analyzing instrument: Iatroscan TLC-FID (MK-6s, Iatron Laboratories) with PeakSimple integration software (ver. 3.67, SRI Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. Lipid classes were determined using thin layer chromatography with flame ionization detection, by micropipetting lipid extracts onto duplicate silica-coated glass rods, developing the rods in a three-stage solvent system to separate lipid classes, scanning the rods on a MK-6s Iatroscan TLC-FID, and integrating the resulting chromatograms using integration software with a calibration curve.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Lu et al. (2008) Can J Fish Aquat Sci 65:2233-2241; Copeman et al. (2017) Mar Ecol Prog Ser 566:183-198.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: total lipids per individual larva; Abbreviation: total.lipid_indiv; Unit: µg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Calculation method and parameters: sum of all lipid class amounts in the lipid sample (TAG, FFA, ST, PL, in ug) divided by number of larvae in lipid sample; Analyzing instrument: Iatroscan TLC-FID (MK-6s, Iatron Laboratories) with PeakSimple integration software (ver. 3.67, SRI Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. Lipid classes were determined using thin layer chromatography with flame ionization detection, by micropipetting lipid extracts onto duplicate silica-coated glass rods, developing the rods in a three-stage solvent system to separate lipid classes, scanning the rods on a MK-6s Iatroscan TLC-FID, and integrating the resulting chromatograms using integration software with a calibration curve.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Lu et al. (2008) Can J Fish Aquat Sci 65:2233-2241; Copeman et al. (2017) Mar Ecol Prog Ser 566:183-198.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: percentage (by weight) of total fatty acids composed of 14:0 (i.e., myristic acid) in the lipid sample; Abbreviation: _14.0; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: chromatogram peak area for 14:0 divided by the sum of all chromatogram peak areas and expressed as a percentage; Analyzing instrument: HP 7890A GC-FID with DB-WAX column and 7693 autosampler (Agilent Technologies, Inc.), and Chem Station software (ver A.01.02, Agilent Technologies, Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. A known quantity of methyl tricosanoate (23:0) was added to the lipid extracts, which were derivatized to form fatty acid methyl esters using acid transesterifcation with sulfuric acid in methanol (Hilditch reagent). Derivatives were analyzed with gas chromatography and the resulting chromatograms were analyzed with integration software.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Budge et al. (2006) Mar Mammal Sci 22:759-801.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: percentage (by weight) of total fatty acids composed of 16:0 (i.e., palmitic acid) in the lipid sample; Abbreviation: _16.0; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: chromatogram peak area for 16:0 divided by the sum of all chromatogram peak areas and expressed as a percentage; Analyzing instrument: HP 7890A GC-FID with DB-WAX column and 7693 autosampler (Agilent Technologies, Inc.), and Chem Station software (ver A.01.02, Agilent Technologies, Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. A known quantity of methyl tricosanoate (23:0) was added to the lipid extracts, which were derivatized to form fatty acid methyl esters using acid transesterifcation with sulfuric acid in methanol (Hilditch reagent). Derivatives were analyzed with gas chromatography and the resulting chromatograms were analyzed with integration software.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Budge et al. (2006) Mar Mammal Sci 22:759-801.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: percentage (by weight) of total fatty acids composed of 16:1n-7 (i.e., palmitoleic acid) in the lipid sample; Abbreviation: 16.1_ n.7; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: chromatogram peak area for 16:0 divided by the sum of all chromatogram peak areas and expressed as a percentage; Analyzing instrument: HP 7890A GC-FID with DB-WAX column and 7693 autosampler (Agilent Technologies, Inc.), and Chem Station software (ver A.01.02, Agilent Technologies, Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. A known quantity of methyl tricosanoate (23:0) was added to the lipid extracts, which were derivatized to form fatty acid methyl esters using acid transesterifcation with sulfuric acid in methanol (Hilditch reagent). Derivatives were analyzed with gas chromatography and the resulting chromatograms were analyzed with integration software.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Budge et al. (2006) Mar Mammal Sci 22:759-801.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: percentage (by weight) of total fatty acids composed of 18:0 (i.e., stearic acid) in the lipid sample; Abbreviation: _18.0; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: chromatogram peak area for 18:0 divided by the sum of all chromatogram peak areas and expressed as a percentage; Analyzing instrument: HP 7890A GC-FID with DB-WAX column and 7693 autosampler (Agilent Technologies, Inc.), and Chem Station software (ver A.01.02, Agilent Technologies, Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. A known quantity of methyl tricosanoate (23:0) was added to the lipid extracts, which were derivatized to form fatty acid methyl esters using acid transesterifcation with sulfuric acid in methanol (Hilditch reagent). Derivatives were analyzed with gas chromatography and the resulting chromatograms were analyzed with integration software.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Budge et al. (2006) Mar Mammal Sci 22:759-801.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: percentage (by weight) of total fatty acids composed of 18:1n-9 (i.e., oleic acid) in the lipid sample; Abbreviation: 18.1_n.9; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: chromatogram peak area for 18:1n-9 divided by the sum of all chromatogram peak areas and expressed as a percentage; Analyzing instrument: HP 7890A GC-FID with DB-WAX column and 7693 autosampler (Agilent Technologies, Inc.), and Chem Station software (ver A.01.02, Agilent Technologies, Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. A known quantity of methyl tricosanoate (23:0) was added to the lipid extracts, which were derivatized to form fatty acid methyl esters using acid transesterifcation with sulfuric acid in methanol (Hilditch reagent). Derivatives were analyzed with gas chromatography and the resulting chromatograms were analyzed with integration software.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Budge et al. (2006) Mar Mammal Sci 22:759-801.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: percentage (by weight) of total fatty acids composed of 18:1n-7 (i.e., vaccenic acid) in the lipid sample; Abbreviation: 18.1_n.7; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: chromatogram peak area for 18:1n-7 divided by the sum of all chromatogram peak areas and expressed as a percentage; Analyzing instrument: HP 7890A GC-FID with DB-WAX column and 7693 autosampler (Agilent Technologies, Inc.), and Chem Station software (ver A.01.02, Agilent Technologies, Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. A known quantity of methyl tricosanoate (23:0) was added to the lipid extracts, which were derivatized to form fatty acid methyl esters using acid transesterifcation with sulfuric acid in methanol (Hilditch reagent). Derivatives were analyzed with gas chromatography and the resulting chromatograms were analyzed with integration software.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Budge et al. (2006) Mar Mammal Sci 22:759-801.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: percentage (by weight) of total fatty acids composed of 18:2n-6 (i.e., linoleic acid) in the lipid sample; Abbreviation: 18.2_n.6; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Analyzing instrument: HP 7890A GC-FID with DB-WAX column and 7693 autosampler (Agilent Technologies, Inc.), and Chem Station software (ver A.01.02, Agilent Technologies, Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. A known quantity of methyl tricosanoate (23:0) was added to the lipid extracts, which were derivatized to form fatty acid methyl esters using acid transesterifcation with sulfuric acid in methanol (Hilditch reagent). Derivatives were analyzed with gas chromatography and the resulting chromatograms were analyzed with integration software.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Budge et al. (2006) Mar Mammal Sci 22:759-801.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: percentage (by weight) of total fatty acids composed of 20:4n-6 (i.e., arachidonic acid) in the lipid sample; Abbreviation: 20.4_n.6; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Calculation method and parameters: chromatogram peak area for 20:4n-6 divided by the sum of all chromatogram peak areas and expressed as a percentage; Analyzing instrument: HP 7890A GC-FID with DB-WAX column and 7693 autosampler (Agilent Technologies, Inc.), and Chem Station software (ver A.01.02, Agilent Technologies, Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. A known quantity of methyl tricosanoate (23:0) was added to the lipid extracts, which were derivatized to form fatty acid methyl esters using acid transesterifcation with sulfuric acid in methanol (Hilditch reagent). Derivatives were analyzed with gas chromatography and the resulting chromatograms were analyzed with integration software.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Budge et al. (2006) Mar Mammal Sci 22:759-801.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: percentage (by weight) of total fatty acids composed of 20:5n-3 (i.e., eicosapentaenoic acid) in the lipid sample; Abbreviation: 20.5_n.3; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: chromatogram peak area for 20:5n-3 divided by the sum of all chromatogram peak areas and expressed as a percentage; Analyzing instrument: HP 7890A GC-FID with DB-WAX column and 7693 autosampler (Agilent Technologies, Inc.), and Chem Station software (ver A.01.02, Agilent Technologies, Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. A known quantity of methyl tricosanoate (23:0) was added to the lipid extracts, which were derivatized to form fatty acid methyl esters using acid transesterifcation with sulfuric acid in methanol (Hilditch reagent). Derivatives were analyzed with gas chromatography and the resulting chromatograms were analyzed with integration software.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Budge et al. (2006) Mar Mammal Sci 22:759-801.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: percentage (by weight) of total fatty acids composed of 22:5n-6 (i.e., osbond acid) in the lipid sample; Abbreviation: 22.5_n.6; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: chromatogram peak area for 22:5n-6 divided by the sum of all chromatogram peak areas and expressed as a percentage; Analyzing instrument: HP 7890A GC-FID with DB-WAX column and 7693 autosampler (Agilent Technologies, Inc.), and Chem Station software (ver A.01.02, Agilent Technologies, Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. A known quantity of methyl tricosanoate (23:0) was added to the lipid extracts, which were derivatized to form fatty acid methyl esters using acid transesterifcation with sulfuric acid in methanol (Hilditch reagent). Derivatives were analyzed with gas chromatography and the resulting chromatograms were analyzed with integration software.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Budge et al. (2006) Mar Mammal Sci 22:759-801.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: percentage (by weight) of total fatty acids composed of 22:5n-3 (i.e., clupanodonic acid) in the lipid sample; Abbreviation: 22.5_n.3; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: chromatogram peak area for 22:5n-3 divided by the sum of all chromatogram peak areas and expressed as a percentage; Analyzing instrument: HP 7890A GC-FID with DB-WAX column and 7693 autosampler (Agilent Technologies, Inc.), and Chem Station software (ver A.01.02, Agilent Technologies, Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. A known quantity of methyl tricosanoate (23:0) was added to the lipid extracts, which were derivatized to form fatty acid methyl esters using acid transesterifcation with sulfuric acid in methanol (Hilditch reagent). Derivatives were analyzed with gas chromatography and the resulting chromatograms were analyzed with integration software.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Budge et al. (2006) Mar Mammal Sci 22:759-801.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: percentage (by weight) of total fatty acids composed of 22:6n-3 (i.e., docosahexaenoic acid) in the lipid sample; Abbreviation: 22.6_n.3; Unit: none; Observation type: none; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: chromatogram peak area for 22:6n-3 divided by the sum of all chromatogram peak areas and expressed as a percentage; Analyzing instrument: HP 7890A GC-FID with DB-WAX column and 7693 autosampler (Agilent Technologies, Inc.), and Chem Station software (ver A.01.02, Agilent Technologies, Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. A known quantity of methyl tricosanoate (23:0) was added to the lipid extracts, which were derivatized to form fatty acid methyl esters using acid transesterifcation with sulfuric acid in methanol (Hilditch reagent). Derivatives were analyzed with gas chromatography and the resulting chromatograms were analyzed with integration software.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Budge et al. (2006) Mar Mammal Sci 22:759-801.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: percentage (by weight) of total fatty acids composed of saturated fatty acids in the lipid sample; Abbreviation: SFA_percent; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: sum of percentages of all saturated fatty acids in the lipid sample; Analyzing instrument: HP 7890A GC-FID with DB-WAX column and 7693 autosampler (Agilent Technologies, Inc.), and Chem Station software (ver A.01.02, Agilent Technologies, Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. A known quantity of methyl tricosanoate (23:0) was added to the lipid extracts, which were derivatized to form fatty acid methyl esters using acid transesterifcation with sulfuric acid in methanol (Hilditch reagent). Derivatives were analyzed with gas chromatography and the resulting chromatograms were analyzed with integration software.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Budge et al. (2006) Mar Mammal Sci 22:759-801.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: percentage (by weight) of total fatty acids composed of monounsaturated fatty acids in the lipid sample; Abbreviation: MUFA_percent; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: sum of percentages of all monounsaturated fatty acids in the lipid sample; Analyzing instrument: HP 7890A GC-FID with DB-WAX column and 7693 autosampler (Agilent Technologies, Inc.), and Chem Station software (ver A.01.02, Agilent Technologies, Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. A known quantity of methyl tricosanoate (23:0) was added to the lipid extracts, which were derivatized to form fatty acid methyl esters using acid transesterifcation with sulfuric acid in methanol (Hilditch reagent). Derivatives were analyzed with gas chromatography and the resulting chromatograms were analyzed with integration software.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Budge et al. (2006) Mar Mammal Sci 22:759-801.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: percentage (by weight) of total fatty acids composed of polyunsaturated fatty acids in the lipid sample; Abbreviation: PUFA_percent; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: sum of percentages of all polyunsaturated fatty acids in the lipid sample; Analyzing instrument: HP 7890A GC-FID with DB-WAX column and 7693 autosampler (Agilent Technologies, Inc.), and Chem Station software (ver A.01.02, Agilent Technologies, Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. A known quantity of methyl tricosanoate (23:0) was added to the lipid extracts, which were derivatized to form fatty acid methyl esters using acid transesterifcation with sulfuric acid in methanol (Hilditch reagent). Derivatives were analyzed with gas chromatography and the resulting chromatograms were analyzed with integration software.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Budge et al. (2006) Mar Mammal Sci 22:759-801.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: categorical life stage of organism; Abbreviation: life_stage; Unit: none; Detailed sampling and analyzing information: coded as "Egg", "Larv", or "Adult".
  • Parameter or Variable: sampling date; Abbreviation: date; Unit: none.
  • Parameter or Variable: time of day of sample collection; Abbreviation: time; Unit: none.
  • Parameter or Variable: seawater sample identification; Abbreviation: bottle_ID; Unit: none.
  • Parameter or Variable: laboratory categorical identifier of seawater sample location; Abbreviation: lab; Unit: none; Detailed sampling and analyzing information: coded as "CR2", "3", or "NAL" to distinguish between distinct locations of seawater samples.
  • Parameter or Variable: CO2 treatment; Abbreviation: CO2_trt; Unit: none; Detailed sampling and analyzing information: categorical identifier coded as "Ambient" or "High" CO2 treatment.
  • Parameter or Variable: binary identifier of experimental 'source' of seawater sample; Abbreviation: Wild_expt; Unit: none; Detailed sampling and analyzing information: coded as: 1 = water from experiment that used eggs and larvae reared from wild-caught eggs, or 0 = water from experiment that used eggs and larvae reared from broodstock.
  • Parameter or Variable: binary identifier of experimental 'source' of seawater sample; Abbreviation: LabSpawn_expt; Unit: none; Detailed sampling and analyzing information: coded as: 1 = water from experiment that used eggs and larvae reared from broodstock, or 0 = water from experiment that used eggs and larvae reared from wild-caught eggs.
  • Parameter or Variable: categorical identifier of seawater sample source; Abbreviation: H.T_ID; Unit: none; Detailed sampling and analyzing information: coded as "1_1", "1_2", "CA_1", "CA_2", "A5", "A6", "L5", "L6", "H1", "H2", "H3", or "H4" to distinguish between distinct sources of seawater samples.
  • Parameter or Variable: larval age in days post hatch when data were collected; Abbreviation: dph; Unit: none.
  • Parameter or Variable: binary identifier of larval age in weeks (post hatch) when data were collected; Abbreviation: week; Unit: none; Detailed sampling and analyzing information: coded as: 1 = 14+ dph, or 2 = 28+ dph.
  • Parameter or Variable: experimental rearing tank identifier; Abbreviation: tank; Unit: none; Detailed sampling and analyzing information: coded as "3", "4", "5", "6", "11", "12", or "13" to distinguish between distinct experimental rearing tanks.
  • Parameter or Variable: time of day when fish were given food; Abbreviation: time_fed; Unit: none.
  • Parameter or Variable: identifier of a tank's larval replicate for each sampling age; Abbreviation: larv_rep; Unit: none.
  • Parameter or Variable: number of larvae used to calculate the means ± standard deviations of each treatment replicate for standard length, body depth, eye diameter, and mean body condition expressed as a winsorized fractional deviation from the ontogenetic relationship between SL & BD; Abbreviation: count_1; Unit: none.
  • Parameter or Variable: number of larvae used to calculate the means ± standard deviations of each treatment replicate for standard length, dry weight, and mean body condition expressed as a fractional deviation from the ontogenetic relationship between SL & DWT; Abbreviation: count_2; Unit: none.
  • Parameter or Variable: lipid sample identification; Abbreviation: lipid_ID; Unit: none.
  • Parameter or Variable: number of larvae used to obtain lipid/fatty acid data; Abbreviation: count_3 .
Acquisition Information (collection)
Instrument
  • laboratory analysis
  • microscope
  • scale
Last Modified: 2024-02-09T01:26:56Z
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