Dataset Overview | National Centers for Environmental Information (NCEI)

Benthic and pelagic invertebrate stable carbon and nitrogen isotope data, Arctic Marine Biodiversity Observing Network (AMBON) research cruise on the Norseman II, from 2017-08-07 to 2017-08-22 (NCEI Accession 0239040)

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This dataset contains benthic and pelagic invertebrate stable carbon and nitrogen isotope data collected in the Chukchi Sea, U.S. Arctic during the 7 August 2017 - 22 August 2017 Arctic Marine Biodiversity Observing Network (AMBON) research cruise aboard the vessel Norseman II. The dataset contains a comma separated values (csv) files exported from Microsoft Excel. These data were generated from samples collected with trawls, grabs, and plankton nets during the research cruise. The data in the file named AMBON2017_Stable Isotopes_Database_final.csv describe the carbon and nitrogen stable isotope values of invertebrate samples with and without various chemical treatments to eliminate carbonates and lipids. Full location and taxonomic information is given for each sample.

Dataset Citation

Cite as: Iken, Katrin (2021). Benthic and pelagic invertebrate stable carbon and nitrogen isotope data, Arctic Marine Biodiversity Observing Network (AMBON) research cruise on the Norseman II, from 2017-08-07 to 2017-08-22 (NCEI Accession 0239040). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/jx3j-4s60. Accessed [date].

Dataset Identifiers

ISO 19115-2 Metadata

gov.noaa.nodc:0239040

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Download Data	HTTPS (download) Navigate directly to the URL for data access and direct download. FTP (download) These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP).
Distribution Formats	CSV
Ordering Instructions	Contact NCEI for other distribution options and instructions.
Distributor	NOAA National Centers for Environmental Information +1-301-713-3277 NCEI.Info@noaa.gov
Dataset Point of Contact	NOAA National Centers for Environmental Information ncei.info@noaa.gov

Time Period	2017-08-07 to 2017-08-22
Spatial Bounding Box Coordinates	West: -168.9543 East: -159.6922 South: 67.668 North: 72.496
Spatial Coverage Map

General Documentation	NCEI Dataset Landing Page Navigate directly to the URL for a descriptive web page with download links. Descriptive Information Navigate directly to the URL for a descriptive web page with download links.
Associated Resources	How to account for the lipid effect on carbon stable-isotope ratio (δ13C): sample treatment effects and model bias https://doi.org/10.1111/j.1095-8649.2007.01754.x journal article

Publication Dates	publication: 2021-07-29
Data Presentation Form	Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status	Complete - production of the data has been completed Historical archive - data has been stored in an offline storage facility
Data Update Frequency	As needed
Supplemental Information	Submission Package ID: DBBNBY
Purpose	The goal of the Arctic Marine Biodiversity Observing Network (AMBON) project is to demonstrate and build an operational marine biodiversity observing network from microbes to whales, integrating diversity levels from genetic to organismal. AMBON is funded through the National Ocean Partnership Program, with contributions from the National Oceanographic and Atmospheric Administration (NOAA), the Bureau of Ocean and Energy Management (BOEM), Shell Exploration and Production Company, and the National Science Foundation (NSF). The AMBON field region is located on the Chukchi Sea continental shelf in the US Arctic as a region exposed to climatic changes and anthropogenic influences. AMBON is composed of a team of multi-institutional and multi-sector partners active in a variety of Arctic biodiversity observing programs and covering disciplines ranging from genetic to organismal to ecosystem. The purpose of this dataset is to describe the stable isotope values of benthic and pelagic invertebrates (with variable treatment to exclude carbonates and lipids) to be used in food web studies and identify carbon sourcing.
Use Limitations	accessLevel: Public Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.

Dataset Citation	Cite as: Iken, Katrin (2021). Benthic and pelagic invertebrate stable carbon and nitrogen isotope data, Arctic Marine Biodiversity Observing Network (AMBON) research cruise on the Norseman II, from 2017-08-07 to 2017-08-22 (NCEI Accession 0239040). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/jx3j-4s60. Accessed [date].
Cited Authors	Iken, Katrin
Principal Investigators	Katrin Iken University of Alaska Fairbanks; College of Fisheries and Ocean Sciences (UAF)
Contributors	University of Alaska Fairbanks; College of Fisheries and Ocean Sciences (UAF)
Resource Providers	Adrienne Canino Axiom Data Science Axiom Data Science
Publishers	NOAA National Centers for Environmental Information
Acknowledgments	Related Funding Agency: National Ocean Partnerships Program Related Funding Agency: National Science Foundation Related Funding Agency: Shell Development Co. Related Funding Agency: National Oceanic and Atmospheric Administration Related Funding Agency: US DOI; Bureau of Ocean Energy Management

Theme keywords	NODC DATA TYPES THESAURUS biological data Carbon content of specimen Nitrogen content of specimen TAXONOMIC CODE ZOOPLANKTON NODC OBSERVATION TYPES THESAURUS laboratory analyses WMO_CategoryCode oceanography Global Change Master Directory (GCMD) Science Keywords EARTH SCIENCE > BIOLOGICAL CLASSIFICATION EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > AQUATIC ECOSYSTEMS > PLANKTON > ZOOPLANKTON EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > STABLE ISOTOPES Provider Data Types CARBON - ORGANIC (Measured) NITROGEN - ORGANIC (Measured)
Data Center keywords	NODC COLLECTING INSTITUTION NAMES THESAURUS University of Alaska Fairbanks; College of Fisheries and Ocean Sciences NODC SUBMITTING INSTITUTION NAMES THESAURUS Axiom Data Science Global Change Master Directory (GCMD) Data Center Keywords Axiom Data Science UAK > University of Alaska, School of Fisheries
Platform keywords	NODC PLATFORM NAMES THESAURUS Norseman II Global Change Master Directory (GCMD) Platform Keywords Ships ICES/SeaDataNet Ship Codes NORSEMAN II (call sign: WDD6688, ICES code: 32QO, 2007)
Instrument keywords	NODC INSTRUMENT TYPES THESAURUS net - zooplankton net trawl Global Change Master Directory (GCMD) Instrument Keywords PLANKTON NETS TRAWL Provider Instruments various
Place keywords	NODC SEA AREA NAMES THESAURUS Chukchi Sea Global Change Master Directory (GCMD) Location Keywords OCEAN > ARCTIC OCEAN > CHUKCHI SEA
Project keywords	Provider Project Names Arctic Marine Biodiversity Ocean Network (AMBON)
Keywords	NCEI ACCESSION NUMBER 0239040

Use Constraints	Cite as: Iken, Katrin (2021). Benthic and pelagic invertebrate stable carbon and nitrogen isotope data, Arctic Marine Biodiversity Observing Network (AMBON) research cruise on the Norseman II, from 2017-08-07 to 2017-08-22 (NCEI Accession 0239040). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/jx3j-4s60. Accessed [date].
Access Constraints	Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
Fees	In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.

Lineage information for: dataset
Processing Steps	2021-07-29T14:38:12Z - NCEI Accession 0239040 v1.1 was published.
Output Datasets	NCEI Accession 0239040 v1.1 NCEI Accession 0239040 v1.1 (download) published 2021-07-29T14:38:12Z

Lineage information for: dataset
Processing Steps	Parameter or Variable: TAXONOMIC CODE (measured); Units: N/A; Observation Category: laboratory analysis; Sampling Instrument: net - zooplankton net; Sampling and Analyzing Method: Dominant and typical meso-zooplankton taxa were collected from 150 µm and 505 µm mesh zooplankton net deployments. Major benthic invertebrate taxa were collected from 0.1 m2 van Veen grabs sieved over 1 mm and a plumb-staff beam trawl with a 4 mm mesh.. Parameter or Variable: NITROGEN - ORGANIC (measured); Units: percent; Observation Category: laboratory analysis; Sampling Instrument: various; Sampling and Analyzing Method: Dominant and typical meso-zooplankton taxa were collected from 150 µm and 505 µm mesh zooplankton net deployments. Major benthic invertebrate taxa were collected from 0.1 m2 van Veen grabs sieved over 1 mm and a plumb-staff beam trawl with a 4 mm mesh. Organisms were sampled as whole organisms (most zooplankton), whole organisms but with the intestinal tract removed (most amphipods), or a slow-turnover tissue such as muscle or body wall was selected. For echinoderms with little muscle tissue, tube feet (Asteroidea) or oral discs (Ophiuroidea) were sampled. Most samples were collected as individuals in replicates of three, but in cases of small individuals, numerous individuals were pooled (e.g., zooplankton) for sufficient mass. Tissue samples were dried at 60°C until constant weight and stored dry until analysis. All taxon names were standardized to the World Register of Marine Species (www.marinespecies.org) Samples were analyzed after one of multiple of the following treatments; many samples were analyzed after several of these treatments to assess treatment effects for the different species: 1.“no treatment” indicates that a dried sample was not chemically treated in any way before isotope analysis; 2.“HCl” indicates a sample was exposed to 1N HCl repeatedly until bubbling from carbonate dissolution ceased to remove isotopically heavier inorganic carbonates from the dietary organic carbon pool (Schlacher & Connolly 2014); 3.“CM” indicates a sample was repeatedly treated with 2:1 chloroform:methanol (CM) to remove 13C-depleted lipids (Mintenbeck et al. 2008) 4.“HCl+CM” indicates samples that were first treated with HCl to remove carbonates and the treated with 2:1 chloroform:methanol to remove lipids. All tissues were rinsed and re-dried after chemical treatment before analysis for carbon and nitrogen stable isotope analysis at the Alaska Stable Isotope Facility (ASIF) at UAF. Stable isotope data for all samples were obtained using continuous-flow isotope ratio mass spectrometry (CF-IRMS). Approximately 0.3-0.5 mg dry weight of each faunal tissue sample were analyzed for delta13C and delta15N values. using a Costech ESC 4010 elemental analyzer interfaced via a Conflo IV with an IRMS (Thermo Finnigan Delta VPlus). Results are expressed as conventional delta (δ) notation in parts per thousand (‰) according to the following equation: delta X = [(R sample/R standard) – 1] • 1000 where X is 13C or 15N of the sample and R is the corresponding ratio 13C/12C or 15N/14N with standards of Vienna Pee Dee Belemnite for delta13C and atmospheric N2 for delta15N. Stable isotope analysis also provided carbon and nitrogen concentrations (as percent) of the sample; these concentrations are given for each measurement as it allows calculation of C;N ratios of samples, which is a useful tool in food web studies and in mathematical corrections of lipid content in samples.; Data Quality Method: delta 13C and delta 15N values accuracy was <0.1 per mil standard deviation. Parameter or Variable: CARBON - ORGANIC (measured); Units: percent; Observation Category: laboratory analysis; Sampling Instrument: various; Sampling and Analyzing Method: Dominant and typical meso-zooplankton taxa were collected from 150 µm and 505 µm mesh zooplankton net deployments. Major benthic invertebrate taxa were collected from 0.1 m2 van Veen grabs sieved over 1 mm and a plumb-staff beam trawl with a 4 mm mesh. Organisms were sampled as whole organisms (most zooplankton), whole organisms but with the intestinal tract removed (most amphipods), or a slow-turnover tissue such as muscle or body wall was selected. For echinoderms with little muscle tissue, tube feet (Asteroidea) or oral discs (Ophiuroidea) were sampled. Most samples were collected as individuals in replicates of three, but in cases of small individuals, numerous individuals were pooled (e.g., zooplankton) for sufficient mass. Tissue samples were dried at 60°C until constant weight and stored dry until analysis. All taxon names were standardized to the World Register of Marine Species (www.marinespecies.org) Samples were analyzed after one of multiple of the following treatments; many samples were analyzed after several of these treatments to assess treatment effects for the different species: 1.“no treatment” indicates that a dried sample was not chemically treated in any way before isotope analysis; 2.“HCl” indicates a sample was exposed to 1N HCl repeatedly until bubbling from carbonate dissolution ceased to remove isotopically heavier inorganic carbonates from the dietary organic carbon pool (Schlacher & Connolly 2014); 3.“CM” indicates a sample was repeatedly treated with 2:1 chloroform:methanol (CM) to remove 13C-depleted lipids (Mintenbeck et al. 2008) 4.“HCl+CM” indicates samples that were first treated with HCl to remove carbonates and the treated with 2:1 chloroform:methanol to remove lipids. All tissues were rinsed and re-dried after chemical treatment before analysis for carbon and nitrogen stable isotope analysis at the Alaska Stable Isotope Facility (ASIF) at UAF. Stable isotope data for all samples were obtained using continuous-flow isotope ratio mass spectrometry (CF-IRMS). Approximately 0.3-0.5 mg dry weight of each faunal tissue sample were analyzed for delta13C and delta15N values. using a Costech ESC 4010 elemental analyzer interfaced via a Conflo IV with an IRMS (Thermo Finnigan Delta VPlus). Results are expressed as conventional delta (δ) notation in parts per thousand (‰) according to the following equation: delta X = [(R sample/R standard) – 1] • 1000 where X is 13C or 15N of the sample and R is the corresponding ratio 13C/12C or 15N/14N with standards of Vienna Pee Dee Belemnite for delta13C and atmospheric N2 for delta15N. Stable isotope analysis also provided carbon and nitrogen concentrations (as percent) of the sample; these concentrations are given for each measurement as it allows calculation of C;N ratios of samples, which is a useful tool in food web studies and in mathematical corrections of lipid content in samples.; Data Quality Method: delta 13C and delta 15N values accuracy was <0.1 per mil standard deviation.

Lineage information for: dataset

Processing Steps

Parameter or Variable: TAXONOMIC CODE (measured); Units: N/A; Observation Category: laboratory analysis; Sampling Instrument: net - zooplankton net; Sampling and Analyzing Method: Dominant and typical meso-zooplankton taxa were collected from 150 µm and 505 µm mesh zooplankton net deployments. Major benthic invertebrate taxa were collected from 0.1 m2 van Veen grabs sieved over 1 mm and a plumb-staff beam trawl with a 4 mm mesh..
Parameter or Variable: NITROGEN - ORGANIC (measured); Units: percent; Observation Category: laboratory analysis; Sampling Instrument: various; Sampling and Analyzing Method: Dominant and typical meso-zooplankton taxa were collected from 150 µm and 505 µm mesh zooplankton net deployments. Major benthic invertebrate taxa were collected from 0.1 m2 van Veen grabs sieved over 1 mm and a plumb-staff beam trawl with a 4 mm mesh. Organisms were sampled as whole organisms (most zooplankton), whole organisms but with the intestinal tract removed (most amphipods), or a slow-turnover tissue such as muscle or body wall was selected. For echinoderms with little muscle tissue, tube feet (Asteroidea) or oral discs (Ophiuroidea) were sampled. Most samples were collected as individuals in replicates of three, but in cases of small individuals, numerous individuals were pooled (e.g., zooplankton) for sufficient mass. Tissue samples were dried at 60°C until constant weight and stored dry until analysis. All taxon names were standardized to the World Register of Marine Species (www.marinespecies.org) Samples were analyzed after one of multiple of the following treatments; many samples were analyzed after several of these treatments to assess treatment effects for the different species: 1.“no treatment” indicates that a dried sample was not chemically treated in any way before isotope analysis; 2.“HCl” indicates a sample was exposed to 1N HCl repeatedly until bubbling from carbonate dissolution ceased to remove isotopically heavier inorganic carbonates from the dietary organic carbon pool (Schlacher & Connolly 2014); 3.“CM” indicates a sample was repeatedly treated with 2:1 chloroform:methanol (CM) to remove 13C-depleted lipids (Mintenbeck et al. 2008) 4.“HCl+CM” indicates samples that were first treated with HCl to remove carbonates and the treated with 2:1 chloroform:methanol to remove lipids. All tissues were rinsed and re-dried after chemical treatment before analysis for carbon and nitrogen stable isotope analysis at the Alaska Stable Isotope Facility (ASIF) at UAF. Stable isotope data for all samples were obtained using continuous-flow isotope ratio mass spectrometry (CF-IRMS). Approximately 0.3-0.5 mg dry weight of each faunal tissue sample were analyzed for delta13C and delta15N values. using a Costech ESC 4010 elemental analyzer interfaced via a Conflo IV with an IRMS (Thermo Finnigan Delta VPlus). Results are expressed as conventional delta (δ) notation in parts per thousand (‰) according to the following equation: delta X = [(R sample/R standard) – 1] • 1000 where X is 13C or 15N of the sample and R is the corresponding ratio 13C/12C or 15N/14N with standards of Vienna Pee Dee Belemnite for delta13C and atmospheric N2 for delta15N. Stable isotope analysis also provided carbon and nitrogen concentrations (as percent) of the sample; these concentrations are given for each measurement as it allows calculation of C;N ratios of samples, which is a useful tool in food web studies and in mathematical corrections of lipid content in samples.; Data Quality Method: delta 13C and delta 15N values accuracy was <0.1 per mil standard deviation.
Parameter or Variable: CARBON - ORGANIC (measured); Units: percent; Observation Category: laboratory analysis; Sampling Instrument: various; Sampling and Analyzing Method: Dominant and typical meso-zooplankton taxa were collected from 150 µm and 505 µm mesh zooplankton net deployments. Major benthic invertebrate taxa were collected from 0.1 m2 van Veen grabs sieved over 1 mm and a plumb-staff beam trawl with a 4 mm mesh. Organisms were sampled as whole organisms (most zooplankton), whole organisms but with the intestinal tract removed (most amphipods), or a slow-turnover tissue such as muscle or body wall was selected. For echinoderms with little muscle tissue, tube feet (Asteroidea) or oral discs (Ophiuroidea) were sampled. Most samples were collected as individuals in replicates of three, but in cases of small individuals, numerous individuals were pooled (e.g., zooplankton) for sufficient mass. Tissue samples were dried at 60°C until constant weight and stored dry until analysis. All taxon names were standardized to the World Register of Marine Species (www.marinespecies.org) Samples were analyzed after one of multiple of the following treatments; many samples were analyzed after several of these treatments to assess treatment effects for the different species: 1.“no treatment” indicates that a dried sample was not chemically treated in any way before isotope analysis; 2.“HCl” indicates a sample was exposed to 1N HCl repeatedly until bubbling from carbonate dissolution ceased to remove isotopically heavier inorganic carbonates from the dietary organic carbon pool (Schlacher & Connolly 2014); 3.“CM” indicates a sample was repeatedly treated with 2:1 chloroform:methanol (CM) to remove 13C-depleted lipids (Mintenbeck et al. 2008) 4.“HCl+CM” indicates samples that were first treated with HCl to remove carbonates and the treated with 2:1 chloroform:methanol to remove lipids. All tissues were rinsed and re-dried after chemical treatment before analysis for carbon and nitrogen stable isotope analysis at the Alaska Stable Isotope Facility (ASIF) at UAF. Stable isotope data for all samples were obtained using continuous-flow isotope ratio mass spectrometry (CF-IRMS). Approximately 0.3-0.5 mg dry weight of each faunal tissue sample were analyzed for delta13C and delta15N values. using a Costech ESC 4010 elemental analyzer interfaced via a Conflo IV with an IRMS (Thermo Finnigan Delta VPlus). Results are expressed as conventional delta (δ) notation in parts per thousand (‰) according to the following equation: delta X = [(R sample/R standard) – 1] • 1000 where X is 13C or 15N of the sample and R is the corresponding ratio 13C/12C or 15N/14N with standards of Vienna Pee Dee Belemnite for delta13C and atmospheric N2 for delta15N. Stable isotope analysis also provided carbon and nitrogen concentrations (as percent) of the sample; these concentrations are given for each measurement as it allows calculation of C;N ratios of samples, which is a useful tool in food web studies and in mathematical corrections of lipid content in samples.; Data Quality Method: delta 13C and delta 15N values accuracy was <0.1 per mil standard deviation.

Acquisition Information (collection)
Instrument	net - zooplankton net trawl
Platform	Norseman II

Last Modified: 2024-06-10T13:19:06Z
For questions about the information on this page, please email: ncei.info@noaa.gov

Benthic and pelagic invertebrate stable carbon and nitrogen isotope data, Arctic Marine Biodiversity Observing Network (AMBON) research cruise on the Norseman II, from 2017-08-07 to 2017-08-22 (NCEI Accession 0239040)

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